158results about How to "Contribute to research" patented technology

The invention provides a preparation method of a V-based alloy metallographic sample, and belongs to the fields of heterogeneous alloy metallographic phase preparation. The method includes: subjecting a block V-based alloy sample to coarse grinding pretreatment, grinding with water step by step, preparing an electrolytic polishing solution, subjecting the sample to electrolytic polishing, subjecting the polished sample to cleaning under ultrasonic wave, wiping with alcohol, blowing the sample to dry the sample, preparing an electrolytic corrosion solution, subjecting the sample to electrolytic corrosion, washing the sample, wiping the sample with alcohol, blowing the sample to dry the sample, and performing metallographic observation. By adjusting experiment parameters and controlling the preparation steps of the V-based alloy metallographic sample, different metallographic phase shapes of the V-based alloy can be obtained, thus meeting observation requirements of different metallographic phases, reflecting comprehensive V-based alloy microstructure and providing experiment support for research of the V-based alloy.

Provided is a simulation test system of a hydraulic support electro-hydraulic control system. The simulation test system comprises a small hydraulic support model, a hydraulic support display board, a simulation device, and a PC server, the small hydraulic support is provided with a plurality of sensors and used for displaying the movement of a hydraulic support and attitude of each part of the support, the hydraulic support display board is used for displaying the movement of the hydraulic support and the states of main sensors in a graphic visual manner, the simulation device is electrically connected with the sensors and used for receiving data of the sensors and performing preliminary processing of the data, and the PC server is electrically connected with the simulation device and used for receiving the data after preliminary processing by the simulation device and performing post-processing of the data and downloading on-site system operation data to a test system and performing reproduction of the on-site operation data and simulation of the system operating environment. According to the simulation test system, the on-site system operation data is collected and downloaded to the test system and is mutually integrated with the simulated test system via a control system, and the laboratory simulation tests in the condition of on-site environment are realized.

The invention relates to a method for realizing the parallel single-pixel imaging, which expands a basic method of the single-pixel imaging into a pixel array (image) sensor and greatly widens the application range of the single-pixel imaging method. The method comprises the following steps of firstly, calculating and positioning an observation area of each pixel on a pixel array by projecting and shooting a pattern mode of a Fourier slice; secondly, projecting a required pattern mode to the scene by using a projector, and shooting by using a camera to obtain a corresponding image; secondly, executing a parallel single-pixel imaging image reconstruction algorithm on all pixels on the camera image to obtain a corresponding image with projector resolution under each camera pixel. Due to the fact that the sparsity during the light transmission process is fully explored, the parallel single-pixel imaging method can greatly improve the imaging efficiency of a traditional single-pixel system. Meanwhile, the corresponding relation between the camera pixels and the projector pixels is established through the reconstruction result, and a good effect is achieved for further promoting the development of the optical imaging theory, the computer graphics and the computer vision.

The invention discloses a preparation method of dendrobium officinale EST-SSR primers. The method comprises the steps of acquiring EST sequences of a dendrobium officinale root; screening dendrobium officinale EST sequences with SSR loci; designing primers; and verifying dendrobium officinale EST-SSR loci to finally obtain 117 pairs of dendrobium officinale EST-SSR labeled primers. The appropriate annealing temperatures of the 117 pairs of dendrobium officinale EST-SSR primers are found to range from 45 DEG C to 63 DEG C, the dendrobium officinale EST-SSR primers are applied to dendrobium officinale genome DNA after being subjected to DNA polymerase chain reaction, and 117 dendrobium officinale DNA sequences with microsatellite loci are obtained through amplification and sequencing confirmation. According to the preparation method, microsatellite molecular markers of dendrobium officinale are developed from the EST sequences, so that the preparation method has the advantages of simplicity, rapidness, stability, high efficiency and low cost. The method provides a powerful tool for molecular identification of dendrobium plants and is beneficial to further study on genetics and molecular biology of the dendrobium plants.

The present invention relates to a mediating method of RNAi (ribonucleic acid interference) utilizing a lentiviral vector. The method comprises the following specific steps of: firstly carrying out homology analysis on the nucleic acid sequence of relevant viruses to find out conserved regions and designing siRNA (small interfering ribonucleic acid) corresponding to the conserved regions of the related viruses; and cloning the siRNA to an expression plasmid pLVTH, then co-transfecting 293T cells together with pCMV-dR8.91 and pMD2.G, collecting the supernatant of the transfected cells, carrying out ultracentrifugation, purification and concentration to obtain a high-titer lentiviral vector expressing the siRNA, then carrying out cotransduction into target cells together with LV-tTR-KRAB, and strictly controlling the acting time and dose of siRNA drugs utilizing tetracycline analogue DOX. Because of high transduction rate and a stringent control system, the mediating method can actually evaluate the in-vivo antiviral action of the siRNA and can track the whole process of drug interference action, thus having clinical application prospects.

The invention relates to the field of gene engineering, and specifically discloses an application of taking a carboxylic acid amides bactericide resistant gene as an oomycetes conversion screening marker. According to the application, coding gene of a mutant protein, which can make oomycetes generate more than 100 times of resistance on a carboxylic acid amides bactericide, is taken as the screening marker and is used to screen the converter in the process of oomycetes conversion. The screening marker can solve the problem that the screening marker is single in genetic transformation of phytophthora capsici and helps the research on functions of gene of phytophthora capsici.

The invention provides a system with a video monitoring function for carrying out research on circadian rhythms under the condition of exposure to microwaves. Various LD (light and dark) lighting systems for the research on the circadian rhythms are automatically implemented by a lighting system setting and illumination control module; a multi-processor working mode is adopted, a main processor notifies a video processor to process corresponding videos via a video processing function calling interface, and is connected with temperature, humidity and light intensity sensors by serial or I2C (inter-integrated circuit) interfaces, data can be acquired and stored, and whether the data exceed set threshold values or not can be judged; and the system supports two modes for controlling microwave radiation, microwave equipment is automatically controlled and measured by LXI standards in one mode, and a microwave generating circuit and a microwave measuring circuit are integrated in the system in the other mode. The system with the video monitoring function for carrying out the research on the circadian rhythms under the condition of exposure to the microwaves has the advantages that an automatic procedure and the lighting system implementation precision can be improved, and the complexity of manual operation is reduced.

The invention relates to a piperazine derivate and application thereof. The piperazine derivate takes N1-arylmethyl and N4-pyridyl piperazine as parent matrixes to modify the structure of pesticides of main pharmacophore of the N1-arylmethyl and N4-pyridyl piperazine, so as to obtain a type of novel compound. As shown by biological (insect disinfestation) activity determination: the novel compound designed and synthesized by the invention can effectively inhibit feeding and growth of crolepidopteron, and cause the crolepidopteron to die. The piperazine derivate not only is helpful to the further research of 5-hydroxytryptamine receptors of insects but also provides direction for creation of green pesticides with a novel functional mechanism.

The invention discloses a construction method for a fat body cell line of a chilo suppressalis larva. The construction method for the fat body cell line of the chilo suppressalis larva includes the following steps: (1) a cell primary culture solution and a cell subculture solution are prepared; (2) a chilo suppressalis larva is soaked in an ethanol solution, surface sterilization is carried out, and the larva body is cleaned and dried through absorption; (3) the larva body is placed in a wax dissection tray disinfected with ethanol, and the chilo suppressalis larva is dissected; (4) a fat body tissue block is placed in a cell culture bottle filled with an HBSS cleaning solution, the cell culture bottle with the fat body tissue block stands still, and the process of pipetting the HBSS and adding fresh HBSS is repeated for three times after fat body cells become adherent; (5) the cell primary culture solution is added, the cell culture bottle is placed into a non-illuminated cell culture box for culture through the night, and then the cell subculture solution is added; (6) the cell subculture solution is pipetted and replaced every 7 days till the culture bottle is filled with expanded and reproduced cells. The construction method for the fat body cell line of the chilo suppressalis larva provided by the invention facilitates chilo suppressalis researches under an isolated culture condition, so as to provide help for the research and development of chilo suppressalis prevention and control methods.

The invention discloses a directional well three-view linkage displaying method. The method comprises the steps of acquiring well post data of a directional well, and analyzing and arranging the well post data, wherein the well post data comprise well post track; setting a projected profile and parameters of each path in the well post; projecting the well post track into three views at the same time, namely the profile track projection, the vertical well post projection and the horizontal well post projection, according to the projected profile and parameters of each path in the well post; conducting interactive operation and linkage display on the three views, namely the profile track projection, the vertical well post projection and the horizontal well post projection, so as to analyze the directional well. By the adoption of the method, three profiles of the directional well can be displayed at the same time in a two-dimension plane, and two-dimension three-view linkage display of the directional well is achieved.

The invention discloses a TDR (time domain reflection) technology based real-time test probe and device for water content of a triaxial sample of unsaturated soil. The probe and the device can be used for testing the local water content of the sample in a triaxial test of the unsaturated soil in geotechnical engineering. The probe comprises a base layer (1), multiple flexible conductors (2) and a connector (3), has certain flexibility, can be horizontally mounted on the cylindrical sample for the triaxial test of geotechnical engineering in a winding manner, is located between the triaxial sample (10) and a rubber membrane (4), is connected with a TDR tester (9) through a wire (8) and can monitor the water content of a mounting part at any time in a triaxial test process of the unsaturated soil. The problem that the water content of a local position cannot be acquired in the triaxial test process of the unsaturated soil in the geotechnical engineering is solved.

The invention belongs to the technical field of engineering testing, and provides a wave velocity testing method and device by a single-hole method and a terminal device. The wave velocity testing method comprises the steps that firstly, a vibration source vibration signal generated by a surface vibration source device at a resonant frequency is obtained, a vibration source vibration waveform is generated, and the resonant frequency is the resonant frequency of a system formed by excitation blocks and strata in the vibration source device; vibration signals of measuring points in boreholes detected by a wave detector are obtained, and a corresponding measuring point vibration waveform is generated; then a wave train figure is obtained according to a mutual correlation analysis method, themeasuring point vibration waveform of the measuring points and the vibration source vibration waveform; and finally, the wave velocity testing results are obtained according to the wave train figure.The measurement of seismic waves is realized, the accuracy of seismic wave measurement in the strata is improved, and the study of the characteristics of foundation soil is facilitated.

The invention discloses an anti-oxidation experimental system for preparing nitrogen at room temperature during laser cladding. The anti-oxidation experimental system is composed of a constant-temperature nitrogen making machine and an anti-oxidation experimental box, wherein the constant-temperature nitrogen making machine comprises an air inlet pipe, a nitrogen-oxygen separation tank, an air outlet pipe, a glass box and an air valve switch, wherein the anti-oxidation experimental box comprises an air delivery pipe, a quartz plate, a box body, a hollow metal base and two small bases. According to the anti-oxidation experimental system for preparing nitrogen during room-temperature cladding disclosed by the invention, nitrogen separated from air can be filled into the anti-oxidation experimental box during laser cladding, so as to prevent a base body and a cladding layer from being oxidized; meanwhile, a few of changes of scientific research personnel and instruments in close observation and laser cladding process detection can be avoided, and thus further research of laser cladding is facilitated. The experimental system also has the advantages of being scientific and reasonable in structure design, simple to use, energy-saving, environmental friendly and the like.

The invention discloses a real-virtual interactive comprehensive experiment platform for a hydraulic mechanism arm, which comprises an upper computer master control system, a motion control system and a hydraulic servo experiment table; wherein the upper computer master control system receives the input control instruction of a user and is connected with the motion control system. Furthermore the upper computer master control system transmits a calculation result to the motion control system. The motion control system receives the instruction of the upper computer mater control system and drives the hydraulic servo experiment table to perform a corresponding action. The real-virtual interactive comprehensive experiment platform performs modular design on an execution mechanism and facilitates detachment. Three-dimensional virtual reality interaction technology is used for realizing real-virtual interaction between the hydraulic experiment table and the mechanical arm. Response characteristics of the servo valve and the execution mechanism can be tested. Execution accuracy of the control algorithm can be verified. The real-virtual interactive comprehensive experiment platform is suitable for research and development of the hydraulic mechanical arm.

The invention discloses a leptobotia elongate four-base repetitive unit simple sequence repeat (SSR) molecular marker establishing method, and an application thereof. The invention belongs to the field of molecular biological DNA marker technology and application. The SSR molecular marker establishing method comprises the steps of: a leptobotia elongate SSR four-base repetitive unit enriched library is established; screening and sequencing are carried out upon SSR sequence positive clones; according to the leptobotia elongate four-base repetitive core sequence, specific primers are designed on two ends; and through PCR amplification detection, 13 pairs of high-polymorphism SSR molecular markers are obtained by screening. The method provided by the invention is mainly applied in respects such as leptobotia elongate resource monitoring, genetic diversity analysis, genetic map construction, molecular-assisted breeding, and the like.

The invention belongs to the technical field of biological data analysis, and relates to a marker screening method based on a single variable and pair variables. As biological data has the advantagesof being high in dimensionality and small in sample size, variables are evaluated and selected through the simple and accurate decision rule, and classification and forecasting are important tasks ofbiological data analysis. For comprehensively evaluating the variables, a best split point of the single variable is calculated through information gain. A new variable is constructed through the bestsplit point, and the pair variables are established through the new variable and an original variable corresponding to the new variable. Meanwhile, variables of primal space generate variable pairs through combination two by two. All the pair variables are scored according to two scoring criterions, and are sorted in descending order according to scores, k pairs of variables which have the highest score and are not overlapped are selected to establish a fusion classifier. According to the marker screening method based on the single variable and the pair variables, the new variable is constructed through the single variable, the classification performance of the single variable and the classification performance of the pair variables can be evaluated through the same rules, and the and effective data processing method is provided.