Method for improving imaging quality of Arabidopsis thaliana seeds

A technology of imaging quality and Arabidopsis thaliana, applied in the field of biology, can solve the problems of poor imaging quality of 3D images and analysis of internal parameters of Arabidopsis thaliana seeds, and achieve the effect of simple processing, remarkable staining effect and clear imaging

Active Publication Date: 2019-01-04
BEIJING FORESTRY UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to solve the problem that the three-dimensional image imaging quality of Arabidopsis thaliana seeds is poor and cannot be used to further analyze the internal parameters of Arabidopsis seeds, and provide a method for improving the imaging quality of Arabidopsis seeds

Method used

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  • Method for improving imaging quality of Arabidopsis thaliana seeds
  • Method for improving imaging quality of Arabidopsis thaliana seeds
  • Method for improving imaging quality of Arabidopsis thaliana seeds

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no. 1 example

[0036] see figure 1 the flow chart of , and Figure 2 to Figure 12 , the method for improving the imaging quality of Arabidopsis thaliana seeds of the present invention is carried out according to the following steps: 1. Preparation of solution: preparing standard fixative solution, taking mass fraction is 37%-40% formaldehyde, glacial acetic acid, volume fraction is 70% -80% alcohol and glycerin are poured into the container according to the volume ratio of 1:(1-2):(10-20):(1-2), and mix well to obtain a standard fixative; prepare cesium iodide solution, weigh Take 8g-12g of cesium iodide powder and dissolve it in 100ml-200ml of distilled water to obtain a cesium iodide solution, which is ready-to-use;

[0037] 2. Treatment of Arabidopsis thaliana seeds: use a pipette to draw cesium iodide solution and standard fixative solution into centrifuge tubes according to the volume ratio of 1: (1-2), blow and suck and mix well; then use pointed tweezers to carefully Cut open the cl...

no. 2 example

[0043] The method for improving the imaging quality of Arabidopsis thaliana seeds of the present invention is different from any embodiment in the specific example one in that the cesium iodide solution is prepared by weighing 10g of cesium iodide powder and dissolved in 100ml of distilled water to obtain iodine cesium chloride solution.

[0044] The method for improving the imaging quality of Arabidopsis thaliana seeds of the present invention is different from the foregoing embodiments in that the siliques are peeled off into the above-mentioned mixed solution, and blown several times with a pipette; they are placed in a vacuum pump, and vacuum dipped at 18kpa for 20min, Then dip in dark at 4°C for 7 days.

specific Embodiment 1

[0047] 1. Preparation of materials: Mature Arabidopsis plant seeds were obtained from Arabidopsis plants grown in an artificial culture room. The conditions of the culture room were relative humidity 80%, constant temperature 24°C, light intensity 180 μmol / m2 / s, and light cycle of 8h dark and 16h light culture;

[0048] Two, preparation solution: the preparation of standard fixative, get formalin (mass fraction is 38% formaldehyde) 5ml, glacial acetic acid 5ml, volume fraction is that 70% alcohol 90ml and 5ml glycerol (glycerin) pour into container Mix evenly to obtain a standard stationary solution; prepare a cesium iodide solution, weigh 10 grams of cesium iodide powder, dissolve it in 100ml of distilled water to obtain a cesium iodide solution, and the cesium iodide solution is prepared for immediate use; instructions are required The most important thing is that the standard fixative is suitable for general root, stem, leaf, anther, and ovary tissue sections. It is widely...

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Abstract

The invention discloses a method for improving the imaging quality of Arabidopsis thaliana seeds, belongs to the field of biology, and particularly relates to the fields of methods for improving the imaging quality of plant seeds. The method aims at solving the problem that the three-dimensional image imaging quality of the Arabidopsis thaliana seeds is poor, and the images cannot be used for further analyzing internal parameters of the Arabidopsis thaliana seeds. The method for improving the imaging quality of the Arabidopsis thaliana seeds comprises the following steps that 1, a solution isprepared, wherein standard stationary liquid is prepared, and a cesium iodide solution is prepared; 2, Arabidopsis thaliana seeds are treated; 3, the Arabidopsis thaliana seeds are dried; 4, micro-calculation tomoscanner imaging of the Arabidopsis thaliana seeds is carried out; 5, a reconstructed picture is preprocessed. Arabidopsis seed images obtained through the method are subjected to Micro CTscanning, the result is clearer, the image quality is improved greatly, setting up of three-dimensional reconstruction of the Arabidopsis thaliana seeds is completely utilized, and reliable data is provided for calculation of the internal parameters of the Arabidopsis thaliana seeds.

Description

technical field [0001] The invention belongs to the field of biology, and in particular relates to the field of a method for improving the imaging quality of plant seeds. Background technique [0002] Arabidopsis is a model plant in plant research, belonging to the Brassicaceae, Angiosperms, and Dicotyledons. The seeds of Arabidopsis thaliana are extremely small, with a thousand-grain weight of about 0.02g. The number of seeds per plant varies with the growth period of each plant and the plant's nutritional conditions. The few ones are about a few hundred, and the many ones can reach 10,000. At present, the research on Arabidopsis seeds mainly focuses on the important role of auxin, abscisic acid, gibberellin and other plant growth hormones and transcription factors in the development and embryonic development of Arabidopsis. In addition, in the study of Arabidopsis seed structure, optical microscopes and electron microscopes are commonly used to observe the internal struct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/046G01N1/30G01N1/28
CPCG01N1/28G01N1/30G01N23/046G01N2223/1016G01N2223/612
Inventor 林金星胡子建张曦杨舜垚单晓昳
Owner BEIJING FORESTRY UNIVERSITY
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