Preparation method of dendrobium officinale primers

A Dendrobium officinale and primer design technology, applied in the field of plant DNA molecular genetic markers, can solve the problems of lack of knowledge of microsatellite molecular markers, etc., and achieve the effect of low cost and high cost

Inactive Publication Date: 2015-01-14
HANGZHOU NORMAL UNIVERSITY
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Problems solved by technology

[0009] The purpose of the present invention is to provide a preparation method of Dendrobium candidum EST-SSR primers, aiming to solve the problem that the understanding of microsatellite molecular markers is very scarce at home and abroad through the development of Dendrobium candidum EST sequences

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  • Preparation method of dendrobium officinale primers
  • Preparation method of dendrobium officinale primers
  • Preparation method of dendrobium officinale primers

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[0031]In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

[0032] figure 1 The flow chart of the preparation method of the Dendrobium officinale EST-SSR primer provided by the present invention is shown. For ease of illustration, only the parts relevant to the present invention are shown.

[0033] The application principle of the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0034] 1. Obtaining the EST sequence of Dendrobium officinale

[0035] In the embodiment of the present invention, a large number of EST sequences of Dendrobium candidum were obtained by constructing an EST library of Dendrobium off...

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Abstract

The invention discloses a preparation method of dendrobium officinale EST-SSR primers. The method comprises the steps of acquiring EST sequences of a dendrobium officinale root; screening dendrobium officinale EST sequences with SSR loci; designing primers; and verifying dendrobium officinale EST-SSR loci to finally obtain 117 pairs of dendrobium officinale EST-SSR labeled primers. The appropriate annealing temperatures of the 117 pairs of dendrobium officinale EST-SSR primers are found to range from 45 DEG C to 63 DEG C, the dendrobium officinale EST-SSR primers are applied to dendrobium officinale genome DNA after being subjected to DNA polymerase chain reaction, and 117 dendrobium officinale DNA sequences with microsatellite loci are obtained through amplification and sequencing confirmation. According to the preparation method, microsatellite molecular markers of dendrobium officinale are developed from the EST sequences, so that the preparation method has the advantages of simplicity, rapidness, stability, high efficiency and low cost. The method provides a powerful tool for molecular identification of dendrobium plants and is beneficial to further study on genetics and molecular biology of the dendrobium plants.

Description

technical field [0001] The invention belongs to the technical field of plant DNA molecular genetic markers, in particular to a preparation method of dendrobium officinale EST-SSR primers. Background technique [0002] Dendrobium (Dendrobium) is the second largest genus of Orchidaceae (Orchidaceae), a perennial herb. It is a kind of commonly used famous and precious traditional Chinese medicine. It was first recorded in "Shen Nong's Materia Medica" and was listed as the top grade. There are more than 1,000 kinds in the world, and there are about 76 kinds in my country, of which nearly 40 kinds are used as medicinal plants. [0003] Dendrobium officinale (Dendrobium officinale) is the ultimate product of Dendrobium, which has unique medicinal value. The whole plant is used as medicine, which has the effects of nourishing yin and stomach, promoting body fluid and quenching thirst. It is mainly distributed in South China and Southwest China. [0004] Dendrobium officinale has a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/10C12Q1/68
Inventor 王慧中卢江杰
Owner HANGZHOU NORMAL UNIVERSITY
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