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Application of tetracyclic triterpenoids in preparation of low-dosage rotifer insecticides

A technology of tetracyclic triterpenes and compounds, which is applied in the field of preparation of low-dose rotifer insecticides by tetracyclic triterpenoids, and can solve the problems of reduced rotifer populations, reduced fecundity rate, and slow filtration speed, etc. , to achieve the effect of less dosage

Active Publication Date: 2015-07-01
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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Problems solved by technology

Therefore, it is only suitable for pre-cultivation treatment, but not for culture systems that have been infected with rotifers during microalgae culture
[0008] 3) Physical methods in the microalgae cultivation process, such as the use of microporous filtration and nylon mesh isolation to control the purity of algae species. This technology can remove large algae-eating animals, but for small algae-eating rotifers and Its eggs are difficult to eradicate, and the filtration speed is slow, which is limited in large-scale applications
[0009] 4) Both rotifers and algae belong to eukaryotes. This characteristic leads to poor effects of simply using drugs (formaldehyde, ammonia, hydrogen peroxide and sodium hypochlorite, etc.) and reducing pH. Control and remove rotifers and their eggs during the treatment process. At the same time, it also causes serious damage to microalgae
[0010] 5) Organophosphorus, carbamic acid and other pesticides. The killing effect of these pesticides on rotifers depends on the effective concentration of poisons, and the impact on the growth and reproduction of microalgae and drug residues on product quality is also not considered.
[0011] In addition, there are specific and selective virus and fungal technical methods. Comps et al. have isolated viruses and fungi (marine double RNA virus and streptochytrium) from dead rotifers in farms, and infected rotifers in the laboratory, proving that These viruses and fungi can lead to reduced rotifer populations and reduced fecundity
The method is expected to be specific, and whether it has application prospects in the large-scale cultivation of microalgae remains to be investigated

Method used

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  • Application of tetracyclic triterpenoids in preparation of low-dosage rotifer insecticides
  • Application of tetracyclic triterpenoids in preparation of low-dosage rotifer insecticides
  • Application of tetracyclic triterpenoids in preparation of low-dosage rotifer insecticides

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] 1. The insecticide that selectively kills rotifers is a kind of tetracyclic triterpenoids extracted from Meliaceae plant Neem Bark and Chuan Neem. Its chemical structure is as follows, specifically:

[0041] The extraction method of tetracyclic triterpenoids can be found in Liu Xiang and Wang Qiu'an, editor in chief, 2010, Natural Products Chemistry. Chapter 2 Extraction and Separation of Natural Products and Structure Identification, Chapter 6 Terpenoids Chapter 2 Extraction and Separation of Terpenoids, that is, plant materials are dried and ground, extracted with benzene or 60% ethanol under hot reflux, and concentrated benzene or After the alcohol solution is added petroleum ether or chloroform for extraction, the tetracyclic triterpenoids are precipitated.

[0042] Formula one

[0043] 2. Use fresh water to dilute the insecticide into a 6mg / L liquid medicine. In order to achieve the best efficacy, use the medicinal solution to be used. If storage is required, the used...

Embodiment 2

[0048] Application in the culture of Pseudochlorococcum: Distilled water is used to prepare 20ml of the insecticide with a concentration of 6mg / L and put it in a 50ml sealed plastic tube, protected from light and low temperature, for later use. The rotifer Brachionus plicatilis is aerated and cultivated with filtered seawater (salinity of 32, pH of 8.2), during which it is fed with Pseudochlorococcum. Take Pseudochlorococcum as the experimental algae species, use L 1 Culture medium to logarithmic growth phase, culture light is 120μmolm -2 s -1 , The photoperiod is D:N=14h:10h. During the experiment, Brachionus plicatilis was added to 700ml of algal cell culture medium at a density of 5 tails per milliliter, and the algal solution without rotifer was used as a blank control group. After 6 hours of adaptation to the rotifers, 0.21ml and 0.25ml of insecticide (6mg / L) were added to make the final concentration 1.7×10 -3 mg / L and 2.1×10 -3 mg / L. At the same time, the algae fluid ad...

Embodiment 3

[0053] Application in the cultivation of chlorella: Prepare 20ml of the used insecticide with a concentration of 6mg / L in distilled water and place it in a 50ml sealed plastic tube, protected from light and low temperature, for later use. The rotifer Brachionus plicatilis is aerated and cultivated with filtered seawater (salinity of 32, pH of 8.2), during which it is fed with Chlorella. Using C146 chlorella in the laboratory as the experimental algae species, using L 1 Culture medium to logarithmic growth phase, culture light is 120μmolm -2 s -1 , The photoperiod is D:N=14h:10h. During the experiment, Brachionus plicatilis was added to 700ml of algal cell culture medium at a density of 4 tails per milliliter, and the algae solution without rotifer was used as a blank control group. After 6 hours of adaptation to the rotifers, 0.21ml and 0.25ml of insecticide (6mg / L) were added to make the final concentration 1.7×10 -3 mg / L and 2.1×10 -3 mg / L. At the same time, the algae liquid...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to application of tetracyclic triterpenoids in preparation of low-dosage rotifer insecticides, and the tetracyclic triterpenoids are shown as the formula I. Based on the difference of basic biological characteristics of phytoplankton and zooplankton, on the basis of experiment, tissues and organs and submicroscopic organelles thereof are chosen as a main target for screening to obtain a plant-sourced drug which is capable of being rapidly degraded and low in toxicity, wherein the tissues and organs only exist in animal body, and not in microalgae, the plant-sourced drug can inhibit gustatory receptors of enemy organisms to cause food refusal reaction so as to act on midintestinal membrane system to seriously damage midintestinal cell structure, reduce the catalytic activity of pepsase and other digestive enzyme systems, influence digestion and absorption functions of the enemy organisms and eventually lead growth and developmental delay or even death. The technique method of the low-dosage rotifer insecticides has the characteristics of less drug use amount, safety, rapidness and effectiveness, and the low-dosage rotifer insecticides is specific on killing and inhibition of zooplankton (such as rotifers).

Description

Technical field [0001] The invention belongs to the field of biotechnology, and specifically is the application of a tetracyclic triterpenoid compound as a low-dose rotifer insecticide. Background technique [0002] As we all know, the microalgae in fresh water and sea water are an important part of the primary productivity in the biosphere, and are also the bait for the growth and development of aquatic animals in the aquatic ecosystem or the bait for the bait organisms. Microalgae (including freshwater and seawater and other microalgae) account for about 50% of plant species; at the same time, microalgae have diversity, complexity and particularity in terms of germplasm, ecological distribution, genetic information, biochemical composition, and metabolic pathways. Determine its potential food, nutrition, health care and pharmaceutical value. In addition, some microalgae can accumulate large amounts of oil under certain conditions, and their oil content even exceeds the oil con...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N43/90A01P15/00
Inventor 刘建国黄园李凌
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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