Method for producing oil preparation of polyhedrosis virus in pine caterpillars character type

Abstract

A kind of production method of pine caterpillar type polyhedrosis virus oil preparation, the viral polyhedron of susceptible pine caterpillar midgut is separated from worm body, and separates from other solid phase substances, then process and concentrate, add solvent ethylene glycol, The suspending agent sodium dodecylbenzene sulfonate is prepared by mixing, stirring and grinding. Its pine caterpillar polyhedrosis virus has high purity, small particle size, and good suspension effect, which solves the problems of sedimentation and layering and blocking nozzles, thereby meeting the requirements of ultra-low volume spraying. The pine caterpillar polyhedrosis virus made according to this method Body virus oil agent, insecticidal rate of more than 92%, its production cost is low, does not pollute the environment, is safe for humans and animals, and has good economic and social benefits.

Description

technical field [0001] The invention relates to a production method of a virus insecticide, in particular to a production method of a pine caterpillar polyhedrosis virus oil. Background technique [0002] Dendrolimus Cytoplasmic polyhedrosis Virus (Dendrolimus Cytoplasmic polyhedrosis Virus, DCPV) is a natural pathogen of pine caterpillars. Dendrolimus Cytoplasmic polyhedrosis Virus has the characteristics of continuous and efficient control of pine caterpillars, is safe and non-toxic to humans and animals, and does not pollute the environment. No waste treatment is required during production. Pine caterpillar polyhedrosis virus is a midgut-infecting virus, which only replicates in the midgut epithelial cells of the host. The worm body is separated and separated from other solid phase substances, then concentrated, and certain auxiliary agents are added to make a dosage form. However, the use effect of the pine caterpillar polyhedrosis virus has a great relationship with i...

AI Technical Summary

Problems solved by technology

Its disadvantages are: due to the blockage of the filter screen, when the filter screen is too fine, it is very difficult to filter, and the gauze screen needs to be replaced and cleaned frequently, which greatly reduces the production capacity and work efficiency of the entire production line

However, because the product contains a large number of particles with large diameters (above 50 microns) such as insect corpse fragments, it can only be made into water or powder for constant spray or powder spray, but it cannot meet the particle fineness requirements of ultra-low volume spray. Require

However, both the water preparation and the powder preparation have some insurmountable shortcomings, which have hindered the large-scale application of the pine caterpillar polyhedrosis virus: the water preparation needs to be diluted with a large amount of clear water when in use, and the amount of liquid sprayed per mu is about 5KG. The labor intensity is high and the operation cost is too high; the drying process of the powder during the manufacturing process seriously affects the activity of the virus, greatly reducing the use effect, and there are problems of sedimentation and blocking of the nozzle during use.