A method for improving the rate of induction of somatic embryos of Pinus massoniana

A technology of induction rate and masson pine, which is applied in the field of plant reproduction, can solve the problems of low induction rate of embryogenic callus, difficulty in obtaining regenerated plants, and difficulty in differentiation, and achieves the ability to maintain normal proliferation, less browning, and acquisition rate of sub-generation. high effect

Active Publication Date: 2021-04-20
GUANGXI FORESTRY RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there have been related studies on somatic embryogenesis of Pinus massoniana, but there are still problems such as low induction rate of embryogenic callus, difficulty in differentiation, low germination rate, and difficulty in obtaining regenerated plants.

Method used

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  • A method for improving the rate of induction of somatic embryos of Pinus massoniana
  • A method for improving the rate of induction of somatic embryos of Pinus massoniana

Examples

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Effect test

Embodiment 1

[0025]On June 25, 2015, the immature cones of Tongmian pine excellent tree that were healthy, free from diseases and insect pests, and the zygotic embryo was in the stage of fission and multiple embryos were collected as explants in the excellent stand of Tongmian pine in the state-owned Paiyangshan Forest Farm in Guangxi. Use volume concentration 45% alcohol + volume concentration 0.03% streptomycin + 100 mg·L -1 After the nicotinic acid mixture was wiped and disinfected on the surface of the explants, put every 4-5 explants into a plastic ziplock bag with a size of 20 mm×30 mm, seal the bag mouth by 80%, fold the bag mouth down Store in a refrigerator at 8°C for 14 days.

[0026] The pretreated explants were taken out of the refrigerator and rinsed with running water for 2 h, soaked in a broad-spectrum fungicide with a volume concentration of 0.1-0.3 % for 1 h, took out the explants and placed them in a volume concentration of 60 % alcohol for 3-5 min, then transferred to 8...

Embodiment 2

[0030] On July 15, 2016, the immature cones of the Tongmian pine excellent tree that were healthy, free from diseases and insect pests, and the zygotic embryos were in the stage of fission and multiple embryos were collected as explants in the excellent stand of Tongmian pine in the state-owned Paiyangshan Forest Farm in Guangxi. Use volume concentration 45% alcohol + volume concentration 0.03% streptomycin + 100 mg·L -1 After the nicotinic acid mixture was wiped and disinfected on the surface of the explants, put every 4-5 explants into a plastic ziplock bag with a size of 20 mm×30 mm, seal the bag mouth by 80%, fold the bag mouth down Store in a refrigerator at 6°C for 12 days.

[0031] The pretreated explants were taken out from the refrigerator and rinsed with running water for 2 h, soaked in a broad-spectrum fungicide with a volume concentration of 0.1-0.3 % for 1.5 h, took out the explants and placed them in a volume concentration of 60 % alcohol for 3-5 min, then trans...

Embodiment 3

[0035] On July 8, 2017, the immature cones of Tongmian pine excellent tree that were healthy, free from diseases and insect pests, and the zygotic embryo was in the stage of fission and polyembryony were collected as explants in the excellent stand of Tongmian pine in the state-owned Paiyangshan Forest Farm in Guangxi. Use volume concentration 45% alcohol + volume concentration 0.03% streptomycin + 100 mg·L -1 After the nicotinic acid mixture was wiped and disinfected on the surface of the explants, put every 4-5 explants into a plastic ziplock bag with a size of 20 mm×30 mm, seal the bag mouth by 80%, fold the bag mouth down Store in a refrigerator at 7°C for 14 days.

[0036]The pretreated explants were taken out of the refrigerator and rinsed with running water for 2 h, soaked in a broad-spectrum fungicide with a volume concentration of 0.1-0.3 % for 1 h, took out the explants and placed them in a volume concentration of 60 % alcohol for 3-5 min, then transferred to a sodi...

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Abstract

The invention provides a method for improving the induction rate of somatic embryos of Pine massoniana, which comprises the steps of explant pretreatment, explant disinfection and somatic embryo induction, and collects healthy Pine massoniana with no pests and diseases and zygotic embryos in the stage of cleavage and multiple embryos. The immature cones of the tree are used as explants. After the explants are pretreated and disinfected, the female gametophytes in the explant cones are aseptically stripped, placed flat on the induction medium and placed in a suitable environment Medium culture, embryogenic callus induction culture period: 3-4 weeks, and finally obtain the embryogenic cell line of Pinus massoniana with rapid growth and vigorous vitality. The present invention undergoes a series of optimized explant treatment and disinfection, and then strips the female gametophytes in the cones for induction, forming a large number of vigorous embryogenic cell lines, which is for further development of Pinus massoniana body embryo seedling breeding, germplasm innovation and biological Engineering breeding has established strong material resources and technical support, and has good economic, social and ecological benefits.

Description

technical field [0001] The invention belongs to the technical field of plant propagation and relates to tissue culture somatic embryogenesis technology, in particular to a method for increasing the induction rate of masson pine somatic embryos. Background technique [0002] Masson pine ( Pinus massoniana Lamb.) is the main tree species used for ecological construction and afforestation in southern my country. As a tree species with high comprehensive utilization value and broad promotion prospects, masson pine can not only be used to produce three-board, papermaking and chemical fiber industrial manufacturing, but also can be used to produce industrial raw materials such as rosin and turpentine. As a large forestry province, Guangxi, according to the "13th Five-Year" forestry development plan of the autonomous region, the development of 100 billion yuan of characteristic and advantageous industries such as turpentine industry and timber industry occupies a pivotal position...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 姚瑞玲王胤
Owner GUANGXI FORESTRY RES INST
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