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Drought-resistant related SSR sequence derived from abnormal cotton and application thereof

An abnormal and drought-resistant technology, applied in the field of SSR sequences related to drought resistance, can solve the problems of limited utilization of cotton diploid wild species, achieve rapid identification accuracy and stability, speed up the breeding process, improve selection efficiency and breeding speed Effect

Active Publication Date: 2020-06-30
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves the efficiency and effectiveness of selecting specific DNA sequences called loci associated with drought tolerance from wild type plants or other sources such as bolls. By combining these methods together, it becomes possible to create crops resistant against water stress caused by drought conditions without having to use expensive chemical treatments like herbicides. Additionally, this method allows researchers to study how different types of plant responses affect crop yield under various environmental factors including temperature variations during precipitation cycles. Overall, this innovation provides technical benefits over existing approaches while reducing costs compared to traditional ways of developing more durable agricultural products.

Problems solved by technology

The technical problem addressed by this patented text relating to improving the ability of plants to resist water stress during different stages of their growth cycle without causing permanent damages or reducing yields caused by excessive environmental factors such as salinity.

Method used

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  • Drought-resistant related SSR sequence derived from abnormal cotton and application thereof
  • Drought-resistant related SSR sequence derived from abnormal cotton and application thereof
  • Drought-resistant related SSR sequence derived from abnormal cotton and application thereof

Examples

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Embodiment 1

[0059] Example 1. Determination of Chromosomal Segments Related to Cotton Drought Resistance and Development of Related Molecular Markers

[0060] Drought-resistant cotton among the present invention is cultivated by the following steps:

[0061] Using upland cotton 86-1 as a donor, crossing with the male abnormal cotton to obtain triploid F 1 (A 1 D. 1 B 1 ), the fertile hexaploid F 1 (A 1 A 1 D. 1 D. 1 B 1 B 1 );

[0062] Using the hexaploid as the female parent, cross with the parent upland cotton Su 8289 to obtain the pentaploid A 1 A 1 D. 1 D. 1 B 1 , plant all received seeds, and obtain 97 individual plants;

[0063] The obtained pentaploid was backcrossed with Su 8289 to obtain BC 2 f 1 The seeds were all planted, and 384 individual plants were obtained, with 50 recombinant types, 36 of which were fertile;

[0064] Will BC 2 f 1 All the individuals of Su 8289 were backcrossed, all planted, and a total of 4331 BC were obtained 3 f 1 Single plant, 4...

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Abstract

The invention discloses a drought-resistant related SSR sequence derived from abnormal cotton and application thereof. The invention provides application of a specific DNA fragment or related biomaterials thereof in cultivation of drought-resistant cotton varieties or strains or improvement of the drought resistance of cotton. The specific DNA fragment is a fragment located on chromosome 5 of abnormal cotton and at least containing a sequence from an SSR molecular marker JAAS6365 (SEQ ID No. 1) to an SSR molecular marker JAAS5604 (SEQ ID No. 2). The related biomaterials are a vector, an expression cassette, recombinant bacteria or transgenic cell line containing the specific DNA fragment. The chromosome fragment provided by the invention has important application value in drought-resistantcotton breeding, and at the same time the SSR molecular marker developed by the invention provides a molecular basis for cultivation of drought-resistant cotton varieties that can be applied to production.

Description

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Claims

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Application Information

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Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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