This invention belongs to the field of
molecular biology technology for
chili pepper breeding, and discloses a
molecular marker, primers, and their applications related to
capsaicin content. The
molecular marker uses the “zhangshugang” version of the
genome as a
reference gene, and its flanking
nucleotide sequence is shown in SEQ ID NO: 1. The primers are shown in SEQ ID NO: 2-4. This invention also provides the primers or kits for identifying and assisting in the screening of
chili pepper spiciness, or for the identification, screening, or breeding of non-spicy
chili pepper fruits. This invention achieves early, accurate, and high-
throughput identification of the “non-spicy” and “spicy” traits in chili peppers. The developed KASP
molecular marker can complete
genotyping within hours using only a small amount of leaf
DNA during the
seedling stage, accurately predicting the spiciness
phenotype of mature fruits, moving the selection process from the field to the laboratory, and breaking through the spatial and temporal limitations of phenotypic identification.