Molecular markers of ssr associated with cotton dwarf and high lint

A molecular marker and lint technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of difficulty in cotton yield, environmental pollution, time-consuming and labor-intensive, and achieve rapid and accurate identification. effect on sex and stability, improving selection efficiency and breeding speed

Active Publication Date: 2022-07-12
JIANGSU ACAD OF AGRI SCI
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  • Claims
  • Application Information

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Problems solved by technology

However, the use of a large amount of chemical reagents such as benzedramine is not only time-consuming and labor-intensive, but also causes a certain degree of pollution to the environment.
[0003] The number of bolls per plant, boll weight and cotton yield are the three major factors that constitute the cotton yield, while the cotton fiber yield is an important factor that has a significant negative correlation with fiber quality, so the traditional breeding methods are used to simultaneously improve Cotton Fiber Quality and Improving Cotton Yield Becomes More Difficult

Method used

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  • Molecular markers of ssr associated with cotton dwarf and high lint
  • Molecular markers of ssr associated with cotton dwarf and high lint
  • Molecular markers of ssr associated with cotton dwarf and high lint

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Embodiment 1

[0051] Example 1. Determination of chromosome fragments related to cotton dwarf and high lint traits and development of related molecular markers

[0052] The short bar high lint cotton in the present invention is cultivated by the following steps:

[0053] Using upland cotton 86-1 as the donor, crossed with the male abnormal cotton to obtain triploid F 1 (A 1 D 1 B 1 ), hexaploid F was obtained by treating the young axillary buds of triploid seedlings with 0.15% colchicine 1 (A 1 A 1 D 1 D 1 B 1 B 1 );

[0054] Taking the hexaploid as the female parent, it was crossed with the parent Su 8289 to obtain the pentaploid A 1 A 1 D 1 D 1 B 1 , planting all the seeds received, resulting in 97 individual plants;

[0055] The obtained pentaploid was backcrossed with Su8289 to obtain BC 2 F 1 The seeds were all planted, and 384 individual plants were obtained, with 50 recombinant types, of which 36 were fertile;

[0056] will BC 2 F 1 All individuals of the 3 F 1...

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Abstract

The invention discloses SSR molecular markers related to cotton dwarf and high lint. The present invention provides a chromosome fragment from abnormal cotton, which is a fragment located on chromosome 11 of abnormal cotton from SSR molecular marker NAU2877 to SSR molecular marker JAAS4311; the nucleotide sequence of the SSR molecular marker NAU2877 is as shown in SEQ ID No. 1; the nucleotide sequence of the SSR molecular marker JAAS4311 is shown in SEQ ID No. 2. The chromosome fragment provided by the invention has important application value in the breeding of dwarf and high-lint cotton, and the SSR molecular marker developed in the invention provides a molecular basis for cultivating dwarf and high-lint cotton varieties that can be used in production.

Description

technical field [0001] The invention relates to the field of cotton breeding, in particular to SSR molecular markers related to cotton dwarf and high lint. Background technique [0002] Cotton is an important cash crop and raw material for the textile industry in my country, and has an irreplaceable position in the national economy. In recent years, with the changes of domestic and foreign economic situation and the adjustment of national industrial policies, the cotton planting area has dropped sharply in the Yangtze River and Yellow River basins. The main reasons are as follows: planting cotton is more time-consuming and labor-intensive than other field crops, and the production cost increases; the acceleration of urbanization and industrialization, the transfer of a large number of labor to cities, and the shortage of rural labor. Therefore, improving the level of mechanization and large-scale production is the only way for the development of cotton production in my coun...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 徐珍珍孟珊沈新莲徐鹏郭琪张香桂
Owner JIANGSU ACAD OF AGRI SCI
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