Specific marker primer pair and identification method for rapid identification of Camellia oleifera Cenruan 2

A technology for an improved camellia seed and an identification method, which is applied in the field of rapid identification of a specific marker primer pair and identification of an improved camellia seed Cenruan No. 2, and can solve the problems of poor repeatability, complex PCR amplification patterns, and low specificity.
CN106676175BActive Publication Date: 2018-03-27GUANGXI FORESTRY RES INST
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGXI FORESTRY RES INST
Publication Date
2018-03-27

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Abstract

The invention discloses a specific labeled primer pair for rapidly identifying a camellia oleifera improved variety Cenruan 2. The specific labeled primer pair comprises an upstream primer and a downstream primer with the base sequences shown as SEQ. ID. No.1 and SEQ. ID. No.2 in a sequence table. Meanwhile, the invention further provides a corresponding identifying method. According to the method, the specific labeled primer pair is taken to perform PCR amplification on camellia oleifera improved variety series, if a DNA band with the length of 756 bp is not shown in an electrophoresis result, the to-be-detected camellia oleifera variety is Cenruan 2, otherwise, the variety is not Cenruan 2. Tests prove that the method has specificity, is only applicable to identification of camellia oleifera improved variety Cenruan series, can perform rapid early identification on the camellia oleifera improved variety Cenruan 2, has the characteristics of being simple, fast, accurate and the like, and is an irreplaceable molecular technique for distinguishing camellia oleifera improved variety on the basis of apparent characteristics.
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Description

technical field

[0001] The invention belongs to the technical field of camellia oleifera identification, and in particular relates to a pair of specific marker primers and an identification method for rapid identification of Camellia oleifera Cenruan 2. Background technique

[0002] Camellia oleifera (Camellia oleifera) is an important woody oil tree species in southern my country, and is known as the world's four largest woody edible oil plants together with oil palm, olive and coconut. Camellia oleifera seed oil is rich in oleic acid, linoleic acid and other unsaturated fatty acids, the content is generally more than 90%, its nutritional value and health value can be compared with olive oil, it is a veritable high-quality edible vegetable oil. Cenxi soft branch Camellia oleifera is the first improved variety of camellia oleifera in my country. It is a farm variety of common camellia oleifera. It is named for its tough and soft branches and drooping fruit. There are 9 high-...

Claims

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