8 results about "Molecular Technique" patented technology

The invention belongs to the technical field of DNA coding molecules, and provides a synthesis method of an on-DNA carbonyl compound, which comprises: providing an on-DNA amino compound and an on-DNA hydroxyl compound, the on-DNA amino compound comprising a DNA fragment and an amino group coupled to the DNA fragment, and the on-DNA hydroxyl compound comprising a DNA fragment and a hydroxyl group coupled to the DNA fragment; and reacting the on-DNA amino compound and / or the on-DNA hydroxyl compound with laccase and an auxiliary catalyst to obtain the on-DNA carbonyl compound, namely the on-DNA carbonyl compound containing a DNA fragment and carbonyl coupled with the DNA fragment. The synthesis method has the advantages of mild conditions, environmental friendliness and small damage to DNA, and is suitable for construction of a DNA coding molecular library, fluorescence labeled molecule coupling performed by using an on-DNA carbonyl reaction handle, DNA modification coupling and functional modification in targeted drug delivery.

The invention provides a preparation method of a self-reflective functional material, which belongs to the technical field of macromolecules, and is characterized in that the self-reflective functional material is prepared by applying the aggregation state characteristics of a macromolecular material, selecting molecular monomers of different systems, and carrying out certain chemical reaction and physical change through different combination proportions and certain media. Therefore, a functional material with local autonomous reflection is prepared.

The invention discloses a nanopore detection probe generated by connecting a target molecule to be detected and a load molecule as well as a preparation method and application of the nanopore detection probe. The nanopore detection probe is obtained by coupling a to-be-detected target molecule and a load molecule through a difunctional cross-linking reagent, wherein the load molecule consists of a guest action molecule, a functional linking group and a traction sequence; the guest action molecule provides a specific signal which is generated by nanometer in a combined detection system with the subject action molecule in the solution; the functional linking group provides an action site of the bifunctional crosslinking reagent, so that the bifunctional crosslinking reagent is combined with a target molecule to be detected; the traction sequence provides power for the whole probe molecule to enter a nanopore detection area under the driving of electrophoretic force. By using the nanopore detection probe, a target molecule to be detected is detected by using a nanopore single molecule technology, so that a characteristic signal is generated to perform qualitative and / or quantitative analysis on the target molecule. By taking amino acid-supported molecules as an example, twenty different amino acids can be distinguished by the method.

The application discloses a nanopore detection probe connected with a target molecule to be detected and a support molecule, and a preparation method and application thereof. The nanopore detection probe is obtained by coupling the target molecule to be detected and the support molecule through a bifunctional cross-linking reagent; wherein the support molecule is composed of a guest interaction molecule, a functional connecting group and a pulling sequence; the guest interaction molecule provides a specific signal for the nanopore generated in a combined detection system of host interaction molecules in a solution; the functional connecting group provides an action site of the bifunctional cross-linking reagent, so as to be combined with the target molecule to be detected; and the pulling sequence provides power for driving the whole probe molecule to enter a nanopore detection area under the action of an electrophoretic force. The target molecule to be detected is detected by using the nanopore detection probe and a nanopore single-molecule technology, so that characteristic signals are generated to perform qualitative and / or quantitative analysis. Taking an amino acid-support molecule as an example, twenty different amino acids can be distinguished by the method.

The invention belongs to the technical field of molecules, and particularly relates to a method for determining the content of cholic acid in in-vitro cultured calculus bovis through ultra-high performance liquid chromatography-triple quadrupole mass spectrometry, which comprises the following steps: S1, preparing a mixed reference substance solution: taking a cholic acid reference substance, dissolving the cholic acid reference substance with methanol, and gradually diluting the cholic acid reference substance into the mixed reference substance solution with a series of concentrations; s2, preparing a to-be-detected sample solution: taking a to-be-detected sample, adding methanol, performing ultrasonic treatment, and complementing the loss weight with methanol; and filtering the supernatant to obtain the to-be-detected sample solution. And S3, UHPLC-MS / MS determination: analyzing the mixed reference substance solution and the to-be-detected sample solution by using a liquid chromatograph-mass spectrometer, and carrying out qualitative and quantitative determination. The method for determining the content of the cholic acid in the in-vitro cultured calculus bovis through ultra-high performance liquid chromatography-triple quadrupole mass spectrometry is simple, convenient and rapid to operate and good in precision, stability and repeatability, 9 cholic acid components in the in-vitro cultured calculus bovis can be detected at the same time, and support can be provided for comprehensive evaluation of the quality of the in-vitro cultured calculus bovis.

The present application belongs to the technical field of molecular biology, and particularly relates to a molecular marker for pigeon sex-linked blue / brown feather color traits and application thereof. The molecular marker is located in the coding region of the TYRP1 gene of a pigeon reference genome GCF_036013475.1, and a deletion mutation of one base T exists in the region; when the site is homozygous non-deletion type, i.e. genotype T / T, or heterozygous deletion type, i.e. genotype T / -, the phenotype is blue feather color; when the site is homozygous deletion type, i.e. genotype - / -, the phenotype is brown feather color. Based on the molecular marker (TYRP1 gene deletion mutation molecular marker), early sex-linked feather color of squab can be accurately identified and feather color purification can be realized, the breeding cycle is significantly shortened, the breeding cost is reduced, and reliable molecular technical support is provided for the cultivation of pigeon self-alternate male and female mating lines.

The invention discloses a circRNA biomarker for forensic medicine PMI inference, a primer pair, a kit and application thereof, and relates to the technical field of gene engineering. The circRNA biomarker is human and mouse circFat3 or a variant with sequence homology greater than or equal to 87%; the primer pair comprises a divergent primer pair and a convergent primer pair, wherein an upstream primer sequence and a downstream primer sequence of the divergent primer pair are as shown in SEQ ID NO: 1 and SEQ ID NO: 2; the upstream primer sequence and the downstream primer sequence of the convergence primer pair are as shown in SEQ ID NO: 3 and SEQ ID NO: 4. The invention further provides a kit for specifically detecting the circFat3 gene and application of the kit in human forensic medicine PMI inference. The circRNA circFat3 is determined as a novel biomarker for PMI inference for the first time, the problems that a traditional forensic medicine method is greatly interfered by the environment and is high in subjectivity are solved through matched primers, a kit and an AI model, and high-precision and standardized molecular technical support is provided for criminal case investigation and civil judicial expertise.

The invention discloses a biological molecule structure prediction method, device and equipment and a medium, and relates to the technical field of biological molecules, and the method comprises the steps: judging whether a received biological molecule structure prediction request contains biological molecule basic information or not; if not, generating corresponding biomolecules by using a preset biomolecule sequence generation algorithm according to biomolecule generation information of the biomolecule structure prediction request, and inputting the biomolecules into the target biomolecule structure prediction tool to obtain a biomolecule structure prediction result; if yes, querying a corresponding biomolecule structure from a preset database based on the biomolecule basic information to obtain a query result; if the query result is that the corresponding biomolecule structure is not queried, inputting the biomolecule basic information into a target biomolecule structure prediction tool to obtain a biomolecule structure prediction result; and if the query result is that the corresponding biomolecular structure is queried, directly outputting the biomolecular structure as a biomolecular structure prediction result.