The invention belongs to the technical field of molecules, and particularly relates to a method for detecting environment DNA of giant salamander distribution. According to the specific technical scheme, according to the method for detecting the environment DNA of giant salamander distribution, at least one pair of primers is used for conducting PCR amplification detection on a to-be-detected sample, if 158-224 bp positive bands appear in an electrophoresis result, it is considered that giant salamanders are distributed in the environment where the to-be-detected sample is sourced, the primer pair is AdCytbE1, and the sequences are SEQ ID NO: 1 and SEQ ID NO: 2. According to the method, environmental DNA of excrement, skin exfoliates, corpses and the like of Chinese giant salamanders in residual water is utilized, a molecular biological technology is combined, a primer with high specificity, high sensitivity and strong universality is developed, and a method capable of carrying out rapid and standardized detection aiming at the distribution of different species of giant salamanders is established.