55 results about "Molecular Technique" patented technology

The invention relates to a method for simultaneous quantification of human nuclear DNA and human male DNA in a biological sample while also detecting the presence of PCR inhibitors in a single reaction. The multiplex quantification method also provides a ratio of human nuclear and male DNA present in a biological sample. Such sample characterization is useful for achieving efficient and accurate results in downstream molecular techniques such as genotyping.

The synthesis of thiophene based conducting polymer molecular actuators, exhibiting electrically triggered molecular conformational transitions is reported. Actuation is believed to be the result of conformational rearrangement of the polymer backbone at the molecular level, not simply ion intercalation in the bulk polymer chain upon electrochemical activation. Molecular actuation results from π-π stacking of thiophene oligomers upon oxidation, producing a reversible molecular displacement that leads to surprising material properties, such as electrically controllable porosity and large strains. The existence of active molecular conformational changes is supported by in situ electrochemical data. Single molecule techniques have been used to characterize the molecular actuators.

The invention relates to the technical field of mRNA molecules, and specifically provides an mRNA and its preparation method and application. The preparation method of the mRNA comprises the following steps: providing a DNA transcription template; the DNA transcription template is composed of a sense strand and an antisense strand, wherein the sense strand comprises a promoter and a target DNA sequence connected to the promoter sequence, and the promoter The subsequence does not contain a stop codon, the target DNA sequence is identical to the target mRNA sequence, and the end of the target DNA sequence is connected with a polyadenylic acid sequence; under the action of RNA polymerase, the DNA transcription template is transcribed in vitro, Under the guidance of the promoter sequence, mRNA is transcribed. The preparation method of the present invention solves the problem of poor molecular homogeneity caused by the increase of by-product sequences during the Poly A tailing reaction of existing mRNA synthesis, and the preparation method also has the advantages of high preparation efficiency and easy purification of products.

The invention relates to application of plasmid capable of being copied in arthrobacter simplex and expressing steroid compound A ring 1, 2 dehydrogenase genes to constructing high-efficiency expression steroid compound A ring 1, 2 dehydrogenation arthrobacter simplex engineering bacteria. The plasmid is capable of being copied in arthrobacter simplex and efficiently expressing steroid compound A ring 1, 2 dehydrogenase genes and significantly practical, and basis is provided for further using plasmid pXJM19 to modify arthrobacter simplex through molecular techniques.

The invention discloses an LAMP (loop-mediated isothermal amplification)-based method for rapid detection of a sclerotinia sclerotiorum strain with high resistance to carbendazim. The method can be used for dynamic monitoring and epidemic warning of field sclerotinia sclerotiorum for a carbendazim resistance group, and is a molecular technique established based on LAMP and used for rapid detection of the sclerotinia sclerotiorum strain with high resistance to the carbendazim. According to the detection method, two pairs of LAMP specific primers are designed on beta-tubulin (comprising 198 amino acid mutation sites) of the sclerotinia sclerotiorum strain with high resistance to the carbendazim, and LAMP is performed; whether the strain is the sclerotinia sclerotiorum strain with high resistance to the carbendazim is determined according to the color of a reaction product; if the color is sky blue (an amplification product exists), the strain is the sclerotinia sclerotiorum strain with high resistance to the carbendazim; and if the color is purple (the amplification product does not exist), the strain is the sclerotinia sclerotiorum strain sensitive to the carbendazim. The method is simple, convenient, fast and low in cost, and has important practical significance for sclerotinia sclerotiorum resistance monitoring and rational drug use.