3 results about "Molecular Technique" patented technology

The application discloses a nanopore detection probe connected with a target molecule to be detected and a support molecule, and a preparation method and application thereof. The nanopore detection probe is obtained by coupling the target molecule to be detected and the support molecule through a bifunctional cross-linking reagent; wherein the support molecule is composed of a guest interaction molecule, a functional connecting group and a pulling sequence; the guest interaction molecule provides a specific signal for the nanopore generated in a combined detection system of host interaction molecules in a solution; the functional connecting group provides an action site of the bifunctional cross-linking reagent, so as to be combined with the target molecule to be detected; and the pulling sequence provides power for driving the whole probe molecule to enter a nanopore detection area under the action of an electrophoretic force. The target molecule to be detected is detected by using the nanopore detection probe and a nanopore single-molecule technology, so that characteristic signals are generated to perform qualitative and / or quantitative analysis. Taking an amino acid-support molecule as an example, twenty different amino acids can be distinguished by the method.

The present application belongs to the technical field of molecular biology, and particularly relates to a molecular marker for pigeon sex-linked blue / brown feather color traits and application thereof. The molecular marker is located in the coding region of the TYRP1 gene of a pigeon reference genome GCF_036013475.1, and a deletion mutation of one base T exists in the region; when the site is homozygous non-deletion type, i.e. genotype T / T, or heterozygous deletion type, i.e. genotype T / -, the phenotype is blue feather color; when the site is homozygous deletion type, i.e. genotype - / -, the phenotype is brown feather color. Based on the molecular marker (TYRP1 gene deletion mutation molecular marker), early sex-linked feather color of squab can be accurately identified and feather color purification can be realized, the breeding cycle is significantly shortened, the breeding cost is reduced, and reliable molecular technical support is provided for the cultivation of pigeon self-alternate male and female mating lines.

The invention discloses a biological molecule structure prediction method, device and equipment and a medium, and relates to the technical field of biological molecules, and the method comprises the steps: judging whether a received biological molecule structure prediction request contains biological molecule basic information or not; if not, generating corresponding biomolecules by using a preset biomolecule sequence generation algorithm according to biomolecule generation information of the biomolecule structure prediction request, and inputting the biomolecules into the target biomolecule structure prediction tool to obtain a biomolecule structure prediction result; if yes, querying a corresponding biomolecule structure from a preset database based on the biomolecule basic information to obtain a query result; if the query result is that the corresponding biomolecule structure is not queried, inputting the biomolecule basic information into a target biomolecule structure prediction tool to obtain a biomolecule structure prediction result; and if the query result is that the corresponding biomolecular structure is queried, directly outputting the biomolecular structure as a biomolecular structure prediction result.