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Application of notoginseng ssr marker in determination of notoginseng saponin r1 content

A technology of Panax notoginseng saponins and markers, applied in the molecular field, can solve the problems of less research on the distribution characteristics of SSR markers in the whole genome of Panax notoginseng

Active Publication Date: 2022-04-05
YUNNAN AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are still few molecular markers for the breeding and genetic improvement of new varieties of Panax notoginseng. The SSR markers developed by EST only analyze a small part of the genome sequence, so there are few studies on the distribution characteristics of SSR markers in the whole genome of Panax notoginseng

Method used

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  • Application of notoginseng ssr marker in determination of notoginseng saponin r1 content
  • Application of notoginseng ssr marker in determination of notoginseng saponin r1 content
  • Application of notoginseng ssr marker in determination of notoginseng saponin r1 content

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Embodiment

[0029] Example: Based on the sequencing information of the Panax notoginseng genome, the present invention mines the SSR site information in the Panax notoginseng genome, develops polymorphic primers, and analyzes the Panax notoginseng (P. notoginseng) and its close wild species P. japonicus , Pingbian notoginseng (P.stipuleanatus), ginger-like notoginseng (P.zingiberensis) and variant narrow-leaved bamboo ginseng (P.japonicus var. angustifolius), wild notoginseng (P.vietnamensis var.fuscidiscus), pearl ginseng ( P.japonicusvar.major).

[0030] The present invention uses different Panax notoginseng germplasm resources as materials for correlation analysis, mainly including Panax notoginseng varieties selected and bred by the group; "Miaoxiang Sanqi No. 1", Kangqi No. 1 "", Dianqi No. 1 ""; Two breeding lines: You 6 and Dwarf stem; and five cultivated populations from Qihe Township, Gucheng District, Lijiang City, Jiujia Township, Zhenyuan County, Pu'er City, and Qiubei County,...

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Abstract

The present invention relates to the field of molecular technology, in particular to the use of notoginseng SSR markers in determining the content of notoginseng saponin R1, and notoginseng saponin R1 is associated with sites SSR35, SSR43, SSR89 and SSR2-6. The invention studies the correlation between the SSR marker and the notoginseng saponin R1, which is beneficial to the development of the notoginseng breeding with high content of the notoginseng saponin R1.

Description

technical field [0001] The invention relates to the field of molecular technology, in particular to the use of SSR markers in determining the content of notoginseng saponin R1. Background technique [0002] Sanqi (Panax notoginseng (Burk.) F.H.Chen) is a perennial herbaceous plant of the genus Panax of the family Araliaceae, distributed in Southwest China. The main components of Panax notoginseng include Panax notoginseng saponins (PNS), notoginseng (notoginseng), flavonoids, volatile oils, amino acids, sugars, etc., which have the functions of hemostasis, cardiomyocyte protection, brain tissue protection, blood fat reduction, and antithrombotic , Enhance immunity, anti-inflammatory, anti-fibrosis, anti-tumor, eliminate oxygen free radicals, anti-oxidation and other pharmacological effects. The spindle root of Panax notoginseng is a well-known special medicine for bruises. It has good effects in stopping bleeding, relieving pain and reducing swelling. The leaves, fruits and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 卢迎春杨生超张广辉刘冠泽赵艳范伟掲应碧李莹
Owner YUNNAN AGRICULTURAL UNIVERSITY
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