Spodoptera frugiperda polymorphic microsatellite molecular markers and primers thereof

Abstract

The invention discloses spodoptera frugiperda polymorphic microsatellite molecular markers and primers thereof. Nucleotide sequences of the microsatellite molecular markers are correspondingly shown as SEQ ID NO.1-12, and primer pairs of the microsatellite molecular marker are shown as SEQ ID NO.13-36. The invention further discloses a preparation method of the polymorphic microsatellite molecularmarker. According to the invention, polymorphism experimental verification is carried out on 33 sites by predicting four-base microsatellite sites with polymorphism in the genome of spodoptera frugiperda, and twelve four-base microsatellite markers with good polymorphism are obtained through screening for the first time, wherein the four-base microsatellite markers comprise four moderate polymorphism markers and eight high polymorphism markers, so that molecular technical support is provided for genetic research on invasion of spodoptera frugiperda, and molecular ecological research on the highly-multiphagous species, namely the grassland spodoptera litura, is facilitated.

Description

technical field [0001] The invention belongs to the technical field of DNA molecular markers of molecular biology, in particular to a polymorphic microsatellite molecular marker of Spodoptera frugiperda and a primer thereof. Background technique [0002] Spodoptera frugiperda, also known as fall armyworm, belongs to the genus Spodoptera of the family Noctuida (Lepidoptera) and is native to tropical and subtropical regions of America (Sparks, 1979). As a polyphagous pest, this insect has a wide range of hosts and can reproduce rapidly and migrate long distances. It has now developed into a major agricultural pest worldwide (Ge Shishuai et al., 2019). The insect invaded Africa in early 2016 and has great potential for further spread and economic damage. It has now spread to 44 countries in Africa, and the occurrence area has exceeded 2.2 million km 2 , has caused serious damage to African agricultural ecosystems (Goergen et al., 2016). Without control measures, fall armyworm ...

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Problems solved by technology

However, studies have shown that the development of microsatellite markers in Lepidoptera insects is relatively difficult, and the proportion of usable microsatellites obtained under the same library construction method is less than that of other species (Meglécz & Solignac, 1998), which is consistent with the characteristics of Lepidoptera insect genomes Related, the abundance of microsatellite sequences in Lepidoptera insects is relatively low (Giajie and Zhang Dexing, 2004); the redundancy of microsatellite flanking regions is high, which are often associated with repetitive sequences, microsatellite families and transposons ( Meglécz et al.,2004; Meglécz et al.,2007; Tay et al.,2010), prone to non-specific amplification of alleles (Pavinato et al.,2013); higher mutations in microsatellite flanking regions Frequency, leading to high null allele frequency, which may lead to failure of PCR amplification (Keyghobadi et al., 1999)

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