20 results about "Electropherogram" patented technology

Provided are a sample analysis method and a sample analysis apparatus capable of specifying a component included in a sample regardless of a concentration of the component included in the sample. A sample analysis method or the like includes a step of separating plural components included in a sample by capillary electrophoresis; a step of obtaining an electropherogram of the sample; a step of acquiring a detection time of each peak and a concentration corresponding to each peak from the electropherogram; and a step of specifying a component included in the sample using a parameter indicating a correlation between a detection time and a concentration calculated using a known component with respect to the acquired detection time and the acquired concentration.

Methods and apparatuses for the identification and / or characterization of properties of a macromolecule based on mass spectrometry data. Specifically, described herein are methods and apparatuses for converting peptide-level data into a pseudo-intact mass spectra. Also described herein are methods and apparatuses for converting peptide-level data into a pseudo-electropherogram. The methods may be well suited for analyzing proteins and protein complexes, including estimating properties of post-translational modifications of the proteins and protein complexes. Methods may include generating a theoretical graph or spectrum based on peptide-level mass spectrometry data. In some embodiments, the theoretical graph may be a theoretical intact mass spectrum or a theoretical charge distribution spectrum.

An analyzer that analyzes one or more pieces of data acquired by electrophoresis separation of a sample includes a memory, a display unit, and a processor. The memory stores the one or more pieces of data. The display unit displays, based on the one or more pieces of data, a gel image, an electropherogram, a first size guide corresponding to the gel image, and a second size guide corresponding to the electropherogram. When the first size guide is located at a first position corresponding to a specific value associated with a size in the gel image, the processor displays a second size guide at a second position corresponding to the specific value in the electropherogram.

To provide a sample analysis method and sample analyzer, capable of specifying a component included in a sample regardless of the concentration of the component included in the sample.SOLUTION: A sample analysis method, etc., comprises steps of: separating a plurality of components included in a sample by capillary electrophoresis; obtaining the electropherogram of the sample; acquiring the detection time of each peak and concentration corresponding to each peak from the electropherogram; and specifying a component included in the sample using parameters showing correlation between the detection time and concentration calculated using known components to the acquired detection time and the acquired concentration.SELECTED DRAWING: Figure 1

Provided is a method for analyzing a sample comprising hemoglobin A2 by capillary electrophoresis using an alkaline aqueous solution comprising a cationic polymer, which is the analysis method including the following steps: 1) obtaining an electropherogram of the sample by capillary electrophoresis; 2) specifying a detection time Tx of an X peak; 3) specifying a detection time Ta2 of an HbA2 peak; 4) specifying a detection time Ty of a Y peak; 5) calculating a correction coefficient from a relative relationship of the detection time Tx, Ta2, and Ty; 6) calculating a ratio of an HbA2 peak area to a total peak area or a ratio of a Y peak area to the total peak area in an electropherogram; and 7) correcting the ratio of the HbA2 peak area or the ratio of the Y peak area with the correction coefficient.

Provided is a data processing device for processing data acquired by an electrophoretic analysis. A graph creator (31, 33) creates an electropherogram and / or a separation image of peaks on an electrophoretic channel. A display processor (37) displays a graph showing the electropherogram and / or the separation image on which a boundary line is drawn to define the inside and the outside of an analysis target range extending along an axis of a separation direction, with the inside and the outside being shown in different visual modes. An analysis condition setter (34) receives an operation by a user for moving the boundary line on the graph displayed and sets the analysis target range whose position and size on the axis are changed according to the received operation. A data-analysis processor (35, 36) carries out a predetermined computation using all or some of peaks included in the analysis target range.

The disclosure is related to methods of determining the concentration of cell-free DNA (cfDNA) and methods of determining the short fragment ratio from a mono-nucleosome by capillary electropherogram. In some embodiments, the determined cfDNA concentration and the short fragment ratio from the mono-nucleosome, optionally with a panel of selected protein biomarkers, can be used for effective and affordable multi-cancer early detection.

The application discloses a method for analyzing epigenetic diversity based on a methylation-sensitive amplified polymorphism (MSAP) gene and a biological gene fingerprint table. The method comprises the following steps: obtaining an MSAP amplification electropherogram of a biological sample to be detected, and constructing a 01 matrix of the sample; converting the 01 matrix into an MSAP gene sequence by using a "CCGGN" replacement method; comparing the MSAP gene sequence with a reference genome, and annotating DNA methylation related gene information; based on the annotation result, constructing a gene fingerprint table based on the copy number of the methylation gene and the methylation / hemimethylation mode, and analyzing epigenetic diversity. The method can convert a traditional MSAP band fingerprint map into a gene fingerprint table with annotation function information without performing cloning and sequencing of MSAP differential fragments, can improve MSAP marker analysis to epigenome analysis, solves the problem that MSAP epigenes are difficult to clone, and improves the level of germplasm resource identification and molecular breeding.

Identification of at least one microorganism present in a sample based on the detection of peptides issued from the cleavage of ribosomal proteins of said microorganism, comprising lysis of microorganism(s) and cleavage of the proteins, obtaining a mixture of peptides, decomplexing the peptide mixture using a liquid separation device coupled with a mass spectrometer, nebulizing the liquid to produce an ion current, receiving the ion current from the ion source, executing on the ion current a series of filtering steps for detecting a transition, receiving data on the transitions, assigning the transitions associated with peptides into two or more contiguous groups, monitoring, and optionally generating a chromatogram or electropherogram, wherein the microorganism is identified according to the detection of said peptide(s).

A computer-implemented method for processing electropherograms provided through a communications network from one or more forensic field test devices to a central communications hub, wherein the forensic field test devices are configured to perform a biological assay analysis, the method implemented using one or more processors programmed with instruction to implement the method comprising receiving, at the central communications hub, one or more electropherograms transmitted through the communication network from the forensic field test devices; scoring each electropherogram based on one or more criteria; placing the one or more electropherograms in a queue for review; matching at least a subset of the electropherograms with each of one or more service providers; based on the matching, transmitting at least the subset to the one or more of the service providers for review; receiving reviewed electropherograms from the one or more service providers; and performing forensic analysis using the reviewed electropherograms.

Systems and methods are disclosed for identifying multiple species in mixed samples using Sanger sequencing. The systems and methods receive a chromatogram from an electropherogram device and generate a plurality of base positions along the chromatogram's time axis. Each base position is generated at either a peak or inflection point in the chromatogram, and multiple base positions are generated at each wide peak in the chromatogram. The systems and methods call a base at the base position. Once bases are called, the systems and methods generate a base calling array that stores called bases in a plurality of indexes. Each index in the plurality of indexes corresponds to a window along the time axis and called bases within the window are added to the corresponding index. Each window partially overlaps with another window, and called bases at base positions that overlap two windows are added to both indexes.

The invention discloses a primer group for identifying the nucleotide diversity of a magnaporthe oryzae avirulence gene AvrPib and application of the primer group, and belongs to the field of gene detection. Four pairs of primers are designed according to three SNP sites of the pyricularia grisea avirulence gene AvrPib, a four-primer amplification hindered mutation system PCR technology is utilized, and different SNP sites of the AvrPib gene are synthesized according to the different SNP sites of the AvrPib gene. The 3'terminal of the primer is located at a nucleotide difference site, the basic group of the site is completely complementary with one genotype and is not matched with the other genotype, and PCR products with different sizes are distinguished through PCR amplification and an electrophoretogram so as to directly identify different genotypes of AvrPib. Group distribution of the field magnaporthe oryzae avirulence gene AvrPib is distinguished through different genotypes, so that rice blast disease resistance breeding and reasonable layout and alternation of varieties in various regions are guided, and a foundation is laid for designing a new strategy for comprehensive control of rice blast.

The invention discloses a multiplex PCR primer group for rapidly detecting oxazolidinone drug resistance genes and a detection method, and belongs to the technical field of molecular biology. The invention designs a multiplex PCR primer group for rapidly detecting the oxazolidinone drug-resistant gene, and the primer sequence is shown as SEQ ID NO.1-8. The multiplex PCR method for detecting the oxazolidinone drug-resistant gene comprises the following steps: firstly, extracting total DNA (deoxyribonucleic acid) of a strain, amplifying the oxazolidinone drug-resistant genes cfr, cfr (D), optrA and poxtA by adopting the multiplex PCR primer group through multiplex PCR reaction, and detecting the oxazolidinone drug-resistant gene by adopting the multiplex PCR primer group to obtain the oxazolidinone drug-resistant gene cfr, cfr (D), optrA and poxtA. And carrying out agarose gel electrophoresis separation on the obtained PCR product, and comparing the obtained PCR product with agarose gel electrophoretograms of cfr, cfr (D), optrA and poxtA genes so as to identify the drug-resistant gene type of the to-be-detected bacteria. Four common oxazolidinone drug-resistant genes (cfr, cfr (D), optrA and poxtA) can be conveniently and rapidly detected from a bacterial sample, compared with single gene PCR, the accuracy, specificity and sensitivity can reach 100%, and the detection efficiency and accuracy are improved.

The invention relates to the technical field of molecular biology and species identification, in particular to a raccoon dog species specific primer pair and application thereof, a specific PCR primer composition for identifying raccoon dog species and application thereof and a method for identifying raccoon dog species. The raccoon dog species specific primer pair is designed based on a raccoon dog mitochondrial control region gene sequence, and is composed of an upstream primer Heid-F1 and a downstream primer Heid-R1. The racoon dog source species identification of a sample can be completed only by interpreting a target band of an electrophoretogram through one-time multiple PCR amplification, and the method has the advantages of being high in timeliness, low in cost and easy and convenient to operate, can be widely applied to the field of population genetics research with a tissue sample as a research object, greatly enriches racoon dog protection biological research means, and has a wide application prospect. And the method has a relatively high application value.

Provided are a sample analysis method and a sample analysis apparatus capable of specifying a component included in a sample regardless of a concentration of the component included in the sample. A sample analysis method or the like includes a step of separating plural components included in a sample by capillary electrophoresis; a step of obtaining an electropherogram of the sample; a step of acquiring a detection time of each peak and a concentration corresponding to each peak from the electropherogram; and a step of specifying a component included in the sample using a parameter indicating a correlation between a detection time and a concentration calculated using a known component with respect to the acquired detection time and the acquired concentration.

The invention discloses a non-cloning genomics analysis method based on a SCoT marker, which can directly restore 01 data into a simulation sequence of PCR (Polymerase Chain Reaction) according to an electrophoretogram by adopting a new data processing and statistical analysis method'primer replacement method 'without cloning and sequencing after completing SCoT electrophoresis on a to-be-detected target. Homologous genes are directly retrieved through bioinformatics analysis of SCoT sequences, classification analysis and function analysis are carried out according to the copy number, existence and distribution of SCoT differential genes, and the molecular basis of genetic diversity variation of genomes is completely revealed. According to the method, SCoT marker analysis is improved into SCoT omics analysis, bioinformatics analysis can be carried out by utilizing genomics big data, a molecular basis of genomic genetic diversity variation among different materials is disclosed, and the method has important significance and wide application prospects in improving the breeding efficiency, accelerating the breeding process and improving the fundamental research theory level.

The application belongs to the technical field of in-vitro detection, and particularly relates to a detection method of endogenous synthesis immunoglobulin in central nervous system based on MALDI-TOF MS. The application provides a kit for detecting and typing immunoglobulin in a sample and a method for detecting immunoglobulin in a sample, and solves the problems of low detection method flux, complicated experimental process, long time consumption, high experience requirement for detection personnel, and result interpretation being easily affected by electropherogram background of isoelectric focusing electrophoresis combined with immunofixed oligoclonal band electrophoresis (OCB) technology. The detection method provided by the application is more accurate, simpler to operate and easier to realize automation, has no subjective problems of manual interpretation, has lower detection cost, has higher detection flux, and is easier to popularize in clinical diagnosis and treatment.

The present invention relates to methods for determining the concentration of cell-free DNA (cfDNA) from capillary electropherogram and calculating the proportion of short fragments from mono-nucleosomes. In some embodiments, the determination of cfDNA concentration and mono-nucleosome short fragment proportion, optionally in combination with a panel of screened protein biomarkers, can be used to achieve efficient and cost-effective early detection of multiple cancer types.

The present invention discloses a human G9 rotavirus HN‑28 strain, which is Reoviridae / Rotavirus A / Human rotavirus (G9P8) HN‑28 Strain, and has been deposited in the China Center for Type Culture Collection on January 13, 2022, with a deposit number of CCTCC NO: V202203. RNA‑PAGE shows that the electrophoresis type of the virus strain is long, and the 11 bands present a unique electrophoresis pattern of group A rotavirus in a 4:2:3:2 arrangement; the whole genome second-generation sequencing results show that this strain is a G9P[8] rotavirus. The present invention successfully isolates a G9 rotavirus wild strain that can be stably propagated through the isolation, culture and cloning and purification of rotavirus-positive fecal samples, named HN‑28 strain, which can be used to prepare rotavirus vaccines.

Various embodiments are described herein related to an assay, method and apparatus for performing an RNA Disruption Assay (RDA) for cellular RNA optionally in response to a cytotoxic treatment such as chemotherapy and / or radiation treatment. The method comprises obtaining at least one electropherogram dataset corresponding to a unique biological sample comprising the cellular RNA at a time point, optionally during or after the treatment; determining values for features from at least two shifted regions of the at least one electropherogram dataset, the shifting being due to the treatment; and optionally determining an RDA score based on a combination of the values of the features.