8 results about "Electropherogram" patented technology

An analyzer that analyzes one or more pieces of data acquired by electrophoresis separation of a sample includes a memory, a display unit, and a processor. The memory stores the one or more pieces of data. The display unit displays, based on the one or more pieces of data, a gel image, an electropherogram, a first size guide corresponding to the gel image, and a second size guide corresponding to the electropherogram. When the first size guide is located at a first position corresponding to a specific value associated with a size in the gel image, the processor displays a second size guide at a second position corresponding to the specific value in the electropherogram.

Provided is a method for analyzing a sample comprising hemoglobin A2 by capillary electrophoresis using an alkaline aqueous solution comprising a cationic polymer, which is the analysis method including the following steps: 1) obtaining an electropherogram of the sample by capillary electrophoresis; 2) specifying a detection time Tx of an X peak; 3) specifying a detection time Ta2 of an HbA2 peak; 4) specifying a detection time Ty of a Y peak; 5) calculating a correction coefficient from a relative relationship of the detection time Tx, Ta2, and Ty; 6) calculating a ratio of an HbA2 peak area to a total peak area or a ratio of a Y peak area to the total peak area in an electropherogram; and 7) correcting the ratio of the HbA2 peak area or the ratio of the Y peak area with the correction coefficient.

The disclosure is related to methods of determining the concentration of cell-free DNA (cfDNA) and methods of determining the short fragment ratio from a mono-nucleosome by capillary electropherogram. In some embodiments, the determined cfDNA concentration and the short fragment ratio from the mono-nucleosome, optionally with a panel of selected protein biomarkers, can be used for effective and affordable multi-cancer early detection.

The application discloses a method for analyzing epigenetic diversity based on a methylation-sensitive amplified polymorphism (MSAP) gene and a biological gene fingerprint table. The method comprises the following steps: obtaining an MSAP amplification electropherogram of a biological sample to be detected, and constructing a 01 matrix of the sample; converting the 01 matrix into an MSAP gene sequence by using a "CCGGN" replacement method; comparing the MSAP gene sequence with a reference genome, and annotating DNA methylation related gene information; based on the annotation result, constructing a gene fingerprint table based on the copy number of the methylation gene and the methylation / hemimethylation mode, and analyzing epigenetic diversity. The method can convert a traditional MSAP band fingerprint map into a gene fingerprint table with annotation function information without performing cloning and sequencing of MSAP differential fragments, can improve MSAP marker analysis to epigenome analysis, solves the problem that MSAP epigenes are difficult to clone, and improves the level of germplasm resource identification and molecular breeding.

The invention relates to the technical field of molecular biology and species identification, in particular to a raccoon dog species specific primer pair and application thereof, a specific PCR primer composition for identifying raccoon dog species and application thereof and a method for identifying raccoon dog species. The raccoon dog species specific primer pair is designed based on a raccoon dog mitochondrial control region gene sequence, and is composed of an upstream primer Heid-F1 and a downstream primer Heid-R1. The racoon dog source species identification of a sample can be completed only by interpreting a target band of an electrophoretogram through one-time multiple PCR amplification, and the method has the advantages of being high in timeliness, low in cost and easy and convenient to operate, can be widely applied to the field of population genetics research with a tissue sample as a research object, greatly enriches racoon dog protection biological research means, and has a wide application prospect. And the method has a relatively high application value.

The application belongs to the technical field of in-vitro detection, and particularly relates to a detection method of endogenous synthesis immunoglobulin in central nervous system based on MALDI-TOF MS. The application provides a kit for detecting and typing immunoglobulin in a sample and a method for detecting immunoglobulin in a sample, and solves the problems of low detection method flux, complicated experimental process, long time consumption, high experience requirement for detection personnel, and result interpretation being easily affected by electropherogram background of isoelectric focusing electrophoresis combined with immunofixed oligoclonal band electrophoresis (OCB) technology. The detection method provided by the application is more accurate, simpler to operate and easier to realize automation, has no subjective problems of manual interpretation, has lower detection cost, has higher detection flux, and is easier to popularize in clinical diagnosis and treatment.

The present invention relates to methods for determining the concentration of cell-free DNA (cfDNA) from capillary electropherogram and calculating the proportion of short fragments from mono-nucleosomes. In some embodiments, the determination of cfDNA concentration and mono-nucleosome short fragment proportion, optionally in combination with a panel of screened protein biomarkers, can be used to achieve efficient and cost-effective early detection of multiple cancer types.

Various embodiments are described herein related to an assay, method and apparatus for performing an RNA Disruption Assay (RDA) for cellular RNA optionally in response to a cytotoxic treatment such as chemotherapy and / or radiation treatment. The method comprises obtaining at least one electropherogram dataset corresponding to a unique biological sample comprising the cellular RNA at a time point, optionally during or after the treatment; determining values for features from at least two shifted regions of the at least one electropherogram dataset, the shifting being due to the treatment; and optionally determining an RDA score based on a combination of the values of the features.