Molecular marking method for identifying tea clonal variety of tea trees

Abstract

Belonging to the field of biotechnologies, the invention provides a molecular marking method for identifying a tea clonal variety of tea trees. The method includes the following steps of: 1) putting 1.0g of fresh and tender buds and leaves of a variety to be tested into a mortar, adding liquid nitrogen and grinding the mixture into powder, adding a CTAB buffer solution, conducting water bathing at a temperature of 65DEG C for 30-60 minutes, then adding a DNA extracting solution to obtain the genome DNA of the tea tree variety to be tested; 2) taking the genome DNA extracted in step 1) as a template, using SSR (simple sequence repeat) primers for PCR amplification, and subjecting the amplification product to 10% native polyacrylamide gel electrophoresis, thus obtaining an electropherogram of the variety to be tested; and 3) comparing the electropherogram of the variety to be tested with a standard tea tree variety electropherogram obtained according to the step 1) and step 2), and if the variety to be tested and the standard variety are consistent in band composition, determining the variety to be tested and the standard variety as an identical variety, thus obtaining the purity of the variety to be tested. Compared with other methods, the invention can reflect the genetic background and genetic relationship of tea tree varieties more truly. And the technology provided in the invention can be used as a scientific basis for identifying the authenticity of the tea tree varieties.

Description

Technical field: [0001] The invention belongs to the field of biotechnology, and relates to a method for identifying the purity of crop varieties, in particular to a molecular marker method for identifying the purity of tea tree clones. Background technique: [0002] Tea is one of the traditional pillar industries in Yunnan and an important source of economic income for farmers in mountainous areas. With the development of famous tea production, all regions are actively promoting the process of improved tea gardens. After years of hard work, the area of ​​cloned improved tea gardens in Yunnan Province has exceeded 1.6 million mu, and the improved variety rate has reached 30%. According to the plan of the Yunnan Provincial Party Committee and the Provincial Government to develop an area of ​​100,000 mu of fine-bred tea gardens each year, 300 million fine-bred seedlings (3,000 per mu) are needed annually. At the same time, with the expansion of fine tea gardens, the developme...

AI Technical Summary

Problems solved by technology

Due to the limitation of technical conditions, the authenticity identification of varieties in the past was mainly based on morphological characteristics and biochemical components, because it is easily affected by environmental conditions, cultivation measures and developmental stages, the reliability is not strong

Due to the lack of effective variety identification means, it is difficult to carry out effective supervision and arbitration when there are counterfeit or disputed varieties in the process of promoting improved varieties

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