32results about How to "The spectrum is clear" patented technology

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The invention discloses an SEM-EDS combined test method for metal foreign matters in a lithium battery material and belongs to the field of lithium ion battery materials. With the method adopted, theproblem of poor detection result accuracy of an existing SEM detection method can be solved. The method comprises the following steps that: high-purity water and a lithium ion positive electrode material are added into a test bottle, a magnet of which the magnetic field intensity ranges from 5000GS to 6000GS is put into the test bottle, and the high-purity water, the lithium ion positive electrodematerial and the magnet are uniformly mixed; a slurry is poured out, the magnet is taken out, a material adhered to the magnet is washed with the high-purity water, a beaker with the magnet arrangedtherein is subjected ultrasonic washing with an ultrasonic instrument, the magnet is taken out from the beaker, is washed and dried; a magnetic foreign matter on the magnet is adhered to a conductiveadhesive tape; and the form and particle size of the magnetic foreign matter particles are identified through an SEM, the chemical composition and type of the foreign matter particles are determined through energy spectrum analysis, and magnetic foreign matter content statistics is performed according to the determined type, quantity and particle size of the metal-containing foreign matter particles. With the method adopted, trace metal foreign matter particles can be accurately analyzed.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

The invention discloses a method for authenticating whether a to-be-detected variety is Ruiza816 and a special primer group thereof. The method provided by the invention comprises the step of carrying out PCR (polymerase chain reaction) amplification by taking the gene group DNA of to-be-detected cotton as a template and by adopting a CS62 primer pair, an NAU1103 primer pair, an NAU1186 primer pair, an NAU1233 primer pair, an NAU2026 primer pair, an NAU2274 primer pair, a CIR062 primer pair, an NAU1071 primer pair, an NAU1085 primer pair, an NAU1102 primer pair, an NAU1196 primer pair, an NAU1255 primer pair, an NAU1369 primer pair, an NAU2173 primer pair and an NAU2277 primer pair, wherein if PCR amplification products meet the requirements of 13 items above from standard (1) to standard (15), the to-be-detected cotton is Ruiza816. According to the invention, the seed production quality of Ruiza816 is monitored, the quality of sold Ruiza816 seeds is ensured, counterfeit Ruiza816 seeds are controlled, the intellectual property of a breeding person is protected, and the variety right is protected.

The invention discloses a method for extracting and separating total proteins of mice sperms by using two-dimensional electrophoresis. The method comprises the following steps: preparing sperms, precipitating DNAs of the sperms after lysis of the sperms, preparing sperm proteins, preparing a protein extraction sample, carrying out first-dimensional isoelectric focusing electrophoresis, carrying out second-dimensional SDS-polyacrylamide gel electrophoresis, dyeing by using coomassie brilliant blue, preparing gel and scanning by using an image scanner. According to the method, lysis of the sperms is completely performed by Trizol, the proteins are extracted by chloroform, impurities are removed by isopropanol, guanidine hydrochloride and ethyl alcohol, and the proteins are purified by acetone, so that the problems of low content of the mice sperms, collection difficulty, small cell volume, a small amount of cytoplasm and a large amount of acrosomal protease are well solved; the sample prepared by the method is complete to prepare, has a large quantity of protein isolating points, and is high in repeatability and clear in chromatogram; by virtue of detection of PDQuestsoftware, the number of the protein points is greater than 1,000; therefore, the method lays the foundation for further mass spectrometry.

Belonging to the field of biotechnologies, the invention provides a molecular marking method for identifying a tea clonal variety of tea trees. The method includes the following steps of: 1) putting 1.0g of fresh and tender buds and leaves of a variety to be tested into a mortar, adding liquid nitrogen and grinding the mixture into powder, adding a CTAB buffer solution, conducting water bathing at a temperature of 65DEG C for 30-60 minutes, then adding a DNA extracting solution to obtain the genome DNA of the tea tree variety to be tested; 2) taking the genome DNA extracted in step 1) as a template, using SSR (simple sequence repeat) primers for PCR amplification, and subjecting the amplification product to 10% native polyacrylamide gel electrophoresis, thus obtaining an electropherogram of the variety to be tested; and 3) comparing the electropherogram of the variety to be tested with a standard tea tree variety electropherogram obtained according to the step 1) and step 2), and if the variety to be tested and the standard variety are consistent in band composition, determining the variety to be tested and the standard variety as an identical variety, thus obtaining the purity of the variety to be tested. Compared with other methods, the invention can reflect the genetic background and genetic relationship of tea tree varieties more truly. And the technology provided in the invention can be used as a scientific basis for identifying the authenticity of the tea tree varieties.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention belongs to the technical field of biology, and discloses an electrophoresis method for removing Rubisco from plant leaves and enriching and separating residual proteins. The method comprises the following steps of: removing Rubisco from watermelon leaves by using 18 percent PEG-4000; leaching residual proteins through a TCA-acetone precipitation method; and performing two-dimensional electrophoresis to separate residual proteins from watermelon leaves. Compared with a holoprotein two-dimensional electrophoretogram, the method has the advantages that: after Rubisco in watermelon leaves is precipitated by adopting the 18 percent PEG, most large and small subunits of Rubisco disappear, the expression levels of most residual proteins are raised, the quantity of proteins obtained by separating is increased by 48.4 percent, and a large quantity of new protein spots (65+/-15) appear in a region of which the molecular weight is smaller than 25.0kDa. As proved by repeated experiments, the electrophoresis method has the advantages of high repeatability, clear spectra and reliable result.

The present invention relates to the field of biotechnology, particularly to special primers for identification or assisted identification of Propionibacterium acnes, and applications thereof, wherein the primers are a primer pair formed by combining any two sequences selected from sequences represented in SEQ ID NO:1-6. According to the present invention, the special primers provide great significance for the species identification of the Propionibacterium acnes and the prevention and control of facial diseases caused by the Propionibacterium acnes infection.

The method of identifying soybean seed authenticity and/or variety purity includes the following steps: mechanically crushing single seed to be detected and adding CTAB buffering liquid; treating in water bath at 65 deg.c for 20-40 min and adding DNA extracting liquid to obtain the genome DNA of each seed; PCR amplification with the extracted genome DNA as template and SSR primer, and performing 6-9 % non-denatured polyacrylamide gel electrophoresis of the PCR amplification product to obtain electropherogram of each seed; and comparing the obtained electropherogram with the standard electropherogram to obtain the soybean seed authenticity and/or variety purity. The method has the features and key technology of SSR marker retained, simplified experiment process, fast identification speed, low cost, accurate result and other advantages.