32results about How to "The spectrum is clear" patented technology

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, the protein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The invention discloses an extraction and dielectrophoresis analytical method for total protein of cattail cauloid, comprising the following steps of: firstly, efficiently extracting protein from the cattail cauloid by screening through many experiments; under a preferred condition, separating the protein components from the secondary metabolite (such as pigments, phenols, quinones and the like) components by a dielectrophoresis method; and performing qualitative analysis by a silver nitrate staining method under a preferred condition, and / or performing qualitative and quantitative analysis on the separated protein by a mass spectrometer. Shown by experimental results, more protein spots are obtained by separation through the dielectrophoresis analytical method, and detected by PDQuest image analysis software, the number of the protein spots is more than 1000, and the spectrum is clear without transverse and longitudinal stripes; and the method has good repeatability and stability, can be used for conveniently identifying the authenticity of the pharmaceutical / food resource plant cattail, and provides a quality control standard and scientific base for in-depth study, scientific planting, culture, development and utilization of cattail.

The invention discloses an SEM-EDS combined test method for metal foreign matters in a lithium battery material and belongs to the field of lithium ion battery materials. With the method adopted, theproblem of poor detection result accuracy of an existing SEM detection method can be solved. The method comprises the following steps that: high-purity water and a lithium ion positive electrode material are added into a test bottle, a magnet of which the magnetic field intensity ranges from 5000GS to 6000GS is put into the test bottle, and the high-purity water, the lithium ion positive electrodematerial and the magnet are uniformly mixed; a slurry is poured out, the magnet is taken out, a material adhered to the magnet is washed with the high-purity water, a beaker with the magnet arrangedtherein is subjected ultrasonic washing with an ultrasonic instrument, the magnet is taken out from the beaker, is washed and dried; a magnetic foreign matter on the magnet is adhered to a conductiveadhesive tape; and the form and particle size of the magnetic foreign matter particles are identified through an SEM, the chemical composition and type of the foreign matter particles are determined through energy spectrum analysis, and magnetic foreign matter content statistics is performed according to the determined type, quantity and particle size of the metal-containing foreign matter particles. With the method adopted, trace metal foreign matter particles can be accurately analyzed.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

The invention discloses a method for authenticating whether a to-be-detected variety is Ruiza816 and a special primer group thereof. The method provided by the invention comprises the step of carrying out PCR (polymerase chain reaction) amplification by taking the gene group DNA of to-be-detected cotton as a template and by adopting a CS62 primer pair, an NAU1103 primer pair, an NAU1186 primer pair, an NAU1233 primer pair, an NAU2026 primer pair, an NAU2274 primer pair, a CIR062 primer pair, an NAU1071 primer pair, an NAU1085 primer pair, an NAU1102 primer pair, an NAU1196 primer pair, an NAU1255 primer pair, an NAU1369 primer pair, an NAU2173 primer pair and an NAU2277 primer pair, wherein if PCR amplification products meet the requirements of 13 items above from standard (1) to standard (15), the to-be-detected cotton is Ruiza816. According to the invention, the seed production quality of Ruiza816 is monitored, the quality of sold Ruiza816 seeds is ensured, counterfeit Ruiza816 seeds are controlled, the intellectual property of a breeding person is protected, and the variety right is protected.

The invention discloses a method for extracting and separating total proteins of mice sperms by using two-dimensional electrophoresis. The method comprises the following steps: preparing sperms, precipitating DNAs of the sperms after lysis of the sperms, preparing sperm proteins, preparing a protein extraction sample, carrying out first-dimensional isoelectric focusing electrophoresis, carrying out second-dimensional SDS-polyacrylamide gel electrophoresis, dyeing by using coomassie brilliant blue, preparing gel and scanning by using an image scanner. According to the method, lysis of the sperms is completely performed by Trizol, the proteins are extracted by chloroform, impurities are removed by isopropanol, guanidine hydrochloride and ethyl alcohol, and the proteins are purified by acetone, so that the problems of low content of the mice sperms, collection difficulty, small cell volume, a small amount of cytoplasm and a large amount of acrosomal protease are well solved; the sample prepared by the method is complete to prepare, has a large quantity of protein isolating points, and is high in repeatability and clear in chromatogram; by virtue of detection of PDQuestsoftware, the number of the protein points is greater than 1,000; therefore, the method lays the foundation for further mass spectrometry.

The invention discloses a method and a special primer set for identifying facticity of to-be-tested variety belonging to CCRI 63. The method provided by the invention comprises the following steps: respectively carrying out PCR (Polymerase Chain Reaction) amplification by use of CIR62, CS62, NAU1070, NAU1102, NAU1186, NAU2026, NAU2173, NAU2277, NAU2343, NAU1196, NAU1085, NAU1255, NAU1369, NAU1233, NAU1269, NAU2272, NAU1187, NAU1375, CS51, NAU1028, NAU2342, NAU868, HAU1300, NAU6094, DPL0238, NAU1362, NAU859, HAU2786, CGR6410 and NAU0478 primers, if a PCR amplification product meets more than 28 standards in the standards of 1)-30), and the to-be-tested cotton is CCRI 63, wherein the genomic DNA of to-be-tested cotton is taken as a template. The method disclosed by the invention can be applied to controlling the quality of cotton seeds, ensuring the quality of commercially available CCRI 63 seeds and fighting against counterfeiting CCRI 63 seeds.

Belonging to the field of biotechnologies, the invention provides a molecular marking method for identifying a tea clonal variety of tea trees. The method includes the following steps of: 1) putting 1.0g of fresh and tender buds and leaves of a variety to be tested into a mortar, adding liquid nitrogen and grinding the mixture into powder, adding a CTAB buffer solution, conducting water bathing at a temperature of 65DEG C for 30-60 minutes, then adding a DNA extracting solution to obtain the genome DNA of the tea tree variety to be tested; 2) taking the genome DNA extracted in step 1) as a template, using SSR (simple sequence repeat) primers for PCR amplification, and subjecting the amplification product to 10% native polyacrylamide gel electrophoresis, thus obtaining an electropherogram of the variety to be tested; and 3) comparing the electropherogram of the variety to be tested with a standard tea tree variety electropherogram obtained according to the step 1) and step 2), and if the variety to be tested and the standard variety are consistent in band composition, determining the variety to be tested and the standard variety as an identical variety, thus obtaining the purity of the variety to be tested. Compared with other methods, the invention can reflect the genetic background and genetic relationship of tea tree varieties more truly. And the technology provided in the invention can be used as a scientific basis for identifying the authenticity of the tea tree varieties.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention discloses a method for preparing a saliva protein sample, and belongs to the technical field of preparation of saliva protein samples. The method is characterized by comprising the following steps of: 1) collecting saliva; 2) centrifuging 800g of collected saliva at 4 DEG C for 15 minutes, and sucking supernate; 3) adding 0.05 to 5 volume percent acetic acid and uniformly mixing; 4) centrifuging 14,000g of the mixed solution at 4 DEG C for 60 minutes, and sucking supernate; 5) putting the supernate in a dialysis bag, and dialyzing by using double distilled water for 5 hours; and 6) condensing to obtain the saliva protein sample. The method has the advantages of high protein recovery rate, short preparation time, safety, nontoxicity, increase of loading quantity of low abundance proteins, clear and easily analyzed maps and the like.

The invention belongs to the technical field of biology, and discloses an electrophoresis method for removing Rubisco from plant leaves and enriching and separating residual proteins. The method comprises the following steps of: removing Rubisco from watermelon leaves by using 18 percent PEG-4000; leaching residual proteins through a TCA-acetone precipitation method; and performing two-dimensional electrophoresis to separate residual proteins from watermelon leaves. Compared with a holoprotein two-dimensional electrophoretogram, the method has the advantages that: after Rubisco in watermelon leaves is precipitated by adopting the 18 percent PEG, most large and small subunits of Rubisco disappear, the expression levels of most residual proteins are raised, the quantity of proteins obtained by separating is increased by 48.4 percent, and a large quantity of new protein spots (65+ / -15) appear in a region of which the molecular weight is smaller than 25.0kDa. As proved by repeated experiments, the electrophoresis method has the advantages of high repeatability, clear spectra and reliable result.

The present invention relates to the field of biotechnology, particularly to special primers for identification or assisted identification of Propionibacterium acnes, and applications thereof, wherein the primers are a primer pair formed by combining any two sequences selected from sequences represented in SEQ ID NO:1-6. According to the present invention, the special primers provide great significance for the species identification of the Propionibacterium acnes and the prevention and control of facial diseases caused by the Propionibacterium acnes infection.

The invention provides a method and a system for constructing and querying a career planning knowledge graph. The method comprises the steps of obtaining recruitment information from a recruitment website, collecting professional classifications and names of undergraduates and specialists, collecting related data of professions corresponding to professions in network data, and collecting employment destinations of graduates of colleges and universities; denoising, classifying, storing and the like are carried out on the obtained data; employing a knowledge graph construction module for constructing a career planning knowledge graph through operations such as named entity recognition; and displaying a result by utilizing the career planning knowledge graph according to the career or screening condition selected by the user at the terminal. According to the invention, the corresponding relation between the career planning knowledge graph and the related professions is obtained through analysis, reference is provided for the user to select the professions, and convenience is provided for the user. A related natural language processing technology is used, so that the efficiency of dataacquisition and data preprocessing is improved; corresponding data is obtained from a recruitment website, and the real-time performance and reliability of the data are guaranteed.

The method of identifying soybean seed authenticity and / or variety purity includes the following steps: mechanically crushing single seed to be detected and adding CTAB buffering liquid; treating in water bath at 65 deg.c for 20-40 min and adding DNA extracting liquid to obtain the genome DNA of each seed; PCR amplification with the extracted genome DNA as template and SSR primer, and performing 6-9 % non-denatured polyacrylamide gel electrophoresis of the PCR amplification product to obtain electropherogram of each seed; and comparing the obtained electropherogram with the standard electropherogram to obtain the soybean seed authenticity and / or variety purity. The method has the features and key technology of SSR marker retained, simplified experiment process, fast identification speed, low cost, accurate result and other advantages.

The invention discloses a method for preparing a saliva protein sample, and belongs to the technical field of preparation of saliva protein samples. The method is characterized by comprising the following steps of: 1) collecting saliva; 2) centrifuging 800g of collected saliva at 4 DEG C for 15 minutes, and sucking supernate; 3) adding 0.05 to 5 volume percent acetic acid and uniformly mixing; 4)centrifuging 14,000g of the mixed solution at 4 DEG C for 60 minutes, and sucking supernate; 5) putting the supernate in a dialysis bag, and dialyzing by using double distilled water for 5 hours; and6) condensing to obtain the saliva protein sample. The method has the advantages of high protein recovery rate, short preparation time, safety, nontoxicity, increase of loading quantity of low abundance proteins, clear and easily analyzed maps and the like.

The invention relates to a construction method of a GC-MS (Gas Chromatography-Mass Spectrometer) fingerprint chromatogram of ice wine, in particular to the construction method of the GC-MS (Gas Chromatography-Mass Spectrometer) fingerprint chromatogram of Wu Nu Mountain ice wine, belonging to the technical field of food analysis. The method is characterized by comprising the following steps of distilling the ice wine, collecting fractions with an initial boiling point of -102DEG C; taking steam of the fractions by using a headspace bottle method; constructing the fingerprint chromatogram by using the GC-MS. Compared with the prior art, the construction method for the fingerprint chromatogram, disclosed by the invention, has the beneficial effects of simplicity, quickness and no need of adding an organic solvent for extracting; the fingerprint chromatogram is high in accuracy, good in repeatability, clear and discernible in chromatogram and great in difference, and can be widely applied to mass discrimination of the Wu Nu Mountain ice wine.

The invention relates to a dimensional electrophoresis method for a total protein of a jute root system, belonging to the field of biotechnology. The total protein of the jute root system capable of existing in the coastal beach is separated by the dimensional electrophoresis technique, and by adopting the technical scheme, the jute root system existing in the coastal beach is tested with good effect. At present, repeated experiments prove that the jute root system is separated by the dimensional electrophoresis method to obtain more protein points; and after detected by PDQuest software, theprotein points are more than 1100, the map is clear without transverse and longitudinal grains, the protein points are circular, and the repeatability is good, thus the invention is a dimensional electrophoresis method suitable for the analysis of the total proteomics of the jute root system.

The invention relates to a method for constructing an HPLC finger-print spectrum of ice wine, in particular to a method for constructing an HPLC finger-print spectrum of Wunvshan ice wine, and belongs to the technical field of foodstuff analysis. According to the technical scheme, the method comprises the steps of distilling the ice wine, collecting fraction from the initial boiling point to 102 DEG C, and constructing the finger-print spectrum with the HPLC. Compared with the prior art, the method for constructing the finger-print spectrum has the advantages that the method is simple and fast, organic solvent is not needed to be added for extraction, the finger-print spectrum is high in accuracy, good in repeatability, clear and legible, great differentiation is achieved, and the method can be widely applied to quality discrimination of the Wunvshan ice wine.

The invention belongs to the technical field of biology, and discloses an electrophoresis method for removing Rubisco from plant leaves and enriching and separating residual proteins. The method comprises the following steps of: removing Rubisco from watermelon leaves by using 18 percent PEG-4000; leaching residual proteins through a TCA-acetone precipitation method; and performing two-dimensional electrophoresis to separate residual proteins from watermelon leaves. Compared with a holoprotein two-dimensional electrophoretogram, the method has the advantages that: after Rubisco in watermelon leaves is precipitated by adopting the 18 percent PEG, most large and small subunits of Rubisco disappear, the expression levels of most residual proteins are raised, the quantity of proteins obtained by separating is increased by 48.4 percent, and a large quantity of new protein spots (65+ / -15) appear in a region of which the molecular weight is smaller than 25.0kDa. As proved by repeated experiments, the electrophoresis method has the advantages of high repeatability, clear spectra and reliable result.

The invention belongs to the field of photoresist resins, and in particular relates to a method for testing photoresist resin components, comprising the following steps: S1, dissolving the photoresist resin to be tested in a solvent, obtaining hydrogen spectra and Carbon spectrum; S2, analyze the hydrogen spectrum and the carbon spectrum, if the monomer peaks in the spectrum do not overlap each other, then get the molar ratio of each monomer in the photoresist resin according to the peak area, if the monomer peaks in the spectrum overlap with each other Overlap, then transfer to S3; S3, perform thermogravimetric analysis on the photoresist resin to be tested, and calculate the molar ratio of each monomer in the photoresist resin. The beneficial effect of the present invention is: when other test methods such as HNMR test, FTIR test will appear that the characteristic peaks overlap together, and the characteristic peaks cannot be distinguished. At this time, thermal analysis can be used as an accurate analysis method to analyze the photoresist resin components.

The invention discloses an ITS-RFLP method for rapidly identifying main cotton pathogenic fungus, which mainly comprises the following steps: 1) extracting cotton main pathogenic fungus mycelia genome DNA, which comprises Verticillium dahliae, Verticillium alboatrum, Rhizoctonia solani, Fusarium moniliforme, Trichothecium roseum and Fusarium oxysporum; 2) amplifying ribosome internal transcribed spacer (ITC) using PCR: carrying out rapid amplification using general primer ITS4 and ITS5 of fungi ITS; 3) carrying out enzyme digestion reaction using restriction enzymes and identifying band spectrums: carrying out single digestion reaction of ITS products of cotton main pathogenic fungus randomly using restriction enzymes HhaI, HaeIII, TaqI and Sau3A whose recognition sites are four basic groups, and comparing ITS-RFLP band spectrum correlation tables and standard electronic maps, thereby rapidly identifying classes of pathogens. Compared with traditional time-consuming and labor-consuming morphological and physiological identification as well as ITS clone sequencing identification method of pathogen with high cost, the invention has the advantages of rapid, accuracy and economy, and simultaneous identifications of a plurality of pathogenic fungus.

The invention relates to the protein two-dimensional electrophoresis technology of a Mongolian ammopiptanthus, which adopts a two-dimensional electrophoresis technology to prepare the protein of a plant living in extreme environment. The experiment carried out to the Xinjiang ammopiptanthus living in the extreme environment by the technical proposal achieves good effect. At present, repeated experiments certify that: the two-dimensional electrophoresis technology has complete prepared laboratory samples, good repeatability, clear atlas and reliable results, can provide technical examples for the research of plant proteomics threatened by adverse circumstances and is a two-dimensional electrophoresis technology applicable to the proteomic analysis of the Xinjiang ammopiptanthus.

The invention discloses technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of the Gastrodia elata Blume and application thereof, and relates to Chinese medicament and natural medicament identification and quality evaluation technology. The invention provides the following key technical parameters for separating and detecting biochemical components of the Gastrodia elata Blume: the optimum mobile phase (acetonitrile solution with gradient concentration), the optimum detection wavelength (219nm) and other basic conditions, and provides the technology for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume and a formula and a method for calculating the content of an effective component gastrodin in the Gastrodia elata Blume. The technology can be used for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume, classifying varieties of the Gastrodia elata Blume and identifying and evaluating the quality of the Gastrodia elata Blume, and also can be used for determining regions suitable for growing the Gastrodia elata Blume and identifying the truth of the Gastrodia elata Blume.

The invention provides an analysis method of pulp proteome in a cucumis melo.L. The method comprises the following steps: extracting pulp protein in the cucumis melo.L, firstly performing isoelectric focusing electrophoresis, and then performing SDS-PAGE gel vertical electrophoresis. The invention provides an economic, simple, easily-implemented and fast method suitable for the extraction and dimensional electrophoresis of total protein in the pulp of the cucumis melo.L. The protein is extracted through a Tris / phenol extraction-methanol / ammonium acetate precipitation method, the protein in the pulp of the cucumis melo.L can be effectively extracted, and the influences from mass sugar and other organic matters are reduced; at present, the repeated experimental results show that the experimental sample is comprehensive in preparation, the 2-DE electrophoresis operation is simple, the graph is clear, the repeatability is good, the reagent is low in toxicity and the result is reliable, a technical example is provided for the research of proteomes of the cucurbitaceae horticultural plants, and the method is suitable for analysis of the pulp proteome in the cucumis melo.L by the dimensional electrophoresis technology.