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Method for identifying soybean seed authenticity and/or purity of variety

A technology for the purity of soybean seeds and varieties, which is applied in biochemical equipment and methods, determination/inspection of microorganisms, electrochemical variables of materials, etc. Complex procedures, etc., to achieve the effect of not easy to store and transport, omit DNA purification and RNA degradation, and simplify experimental steps

Inactive Publication Date: 2008-07-30
BEIJING UNIV OF AGRI
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Problems solved by technology

However, the currently used SSR molecular marker technology has complex procedures, high technical content and high testing costs, and direct transplantation for the identification of seed authenticity and variety purity is not easily accepted by seed production and business units

Method used

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  • Method for identifying soybean seed authenticity and/or purity of variety

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Embodiment 1

[0077] Embodiment 1, take Jinda 75 seeds as standard seed identification soybean seed authenticity and variety purity

[0078] Before the identification, more than 20 soybean varieties (including Jinda 75) from Shanxi Province were screened for SSR markers, and it was found that the SSR primer Sat_344 could produce a specific band for the genomic DNA of Jinda 75. The DNA of Jinda 75 samples for inspection was analyzed.

[0079] 1. Seed sample selection

[0080] 95 seeds were randomly selected from the soybean variety Jinda 75, and 3 seeds of Jinda 74 and 2 seeds of Jinda 701 were added to test the identification effect, and a total of 100 seeds were used as test samples.

[0081] 2. DNA Extraction from Seed Samples

[0082] (1) Crush the soybean seeds one by one with pliers, put each in a 1.5ml centrifuge tube, and the sample occupies about 1 / 3 of the volume of the centrifuge tube.

[0083] (2) Add 600 μl of CTAB buffer solution to each 1.5ml centrifuge tube, and bathe in w...

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Abstract

The method of identifying soybean seed authenticity and / or variety purity includes the following steps: mechanically crushing single seed to be detected and adding CTAB buffering liquid; treating in water bath at 65 deg.c for 20-40 min and adding DNA extracting liquid to obtain the genome DNA of each seed; PCR amplification with the extracted genome DNA as template and SSR primer, and performing 6-9 % non-denatured polyacrylamide gel electrophoresis of the PCR amplification product to obtain electropherogram of each seed; and comparing the obtained electropherogram with the standard electropherogram to obtain the soybean seed authenticity and / or variety purity. The method has the features and key technology of SSR marker retained, simplified experiment process, fast identification speed, low cost, accurate result and other advantages.

Description

technical field [0001] The invention relates to a method for identifying the authenticity and / or variety purity of crop seeds, in particular to a method for identifying the authenticity and / or variety purity of soybean seeds. Background technique [0002] Seed is a special product of life, and seed inspection is the main means for comprehensive control and evaluation of seed quality. In recent years, my country has promulgated a series of laws and regulations to guide the work of crop seeds, such as "Crop Seed Inspection Regulations" (National Standard of the People's Republic of China, GB / T 3543.1~3543.7-1995), "People's Republic of China New Plant Variety Protection "Regulations" (1997), "Seed Law of the People's Republic of China" (2000), etc. The purpose is to ensure the safe use of seeds in agricultural production and protect the legitimate rights and interests of seed users. In my country's "Crop Seed Inspection Regulations", it is stipulated that seed clarity analysi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68G01N27/26
Inventor 谢皓陈学珍于同泉秦岭路苹杨柳王建立冯永庆南张杰
Owner BEIJING UNIV OF AGRI
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