Technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of Gastrodia elata Blume

A fingerprint and quality identification technology, applied in the field of biochemical fingerprint classification, quality identification and evaluation of Gastrodia elata, can solve the problems of low gastrodin content, lack of practicability, and few biochemical components of Gastrodia elata, and achieves clear spectrum and large peak area. , the effect of high sensitivity

Active Publication Date: 2013-04-17
西藏和藤医药发展股份有限公司
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  • Description
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  • Application Information

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Problems solved by technology

However, these methods take a long time to determine the biochemical fingerprint of Gastrodia elata, and the detected content of gastrodin is low, which lacks practicability, especially cannot be used to classify and evaluate Gastrodia elata
The Gastrodia elata chemical fingerprint identification technology invented by Tao Jun et al. has poor separation effect and few biochemical components of Gastrodia elata can be detected

Method used

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  • Technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of Gastrodia elata Blume
  • Technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of Gastrodia elata Blume
  • Technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of Gastrodia elata Blume

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Embodiment 1: The present invention is used for measuring the biochemical fingerprint of various Gastrodia elata:

[0052] The fresh Gastrodia elata samples were steamed, dried, ground into powder by traditional methods, passed through an 80-mesh sieve, and dried in a drying oven at 80°C for 1 hour. Accurately weigh 1 g of Gastrodia elata powder with an electronic balance, put it in a conical flask, and add 70% ethanol solution. Connect the triangular flask to a Soxhlet extractor, reflux it in hot water at 97°C for 20 minutes, and treat it with a JY92-2D ultrasonic cell disruptor for 25 minutes (sonication for 9 seconds; pause for 2 seconds; amplitude power 250w). Weigh after sonication, and replace the lost weight with 70% ethanol solution. Repeat the above process once. The extract solution was filtered with filter paper. The filtrate was collected, evaporated, concentrated and dried, and fixedly dissolved to 10 ml with 10% acetonitrile solution (mobile phase). Af...

Embodiment 2

[0053] Embodiment 2, the present invention is used for Gastrodia elata classification

[0054] By determining the biochemical fingerprint of Gastrodia elata samples, as described above. Then, Gastrodia elata was classified according to the shape features and the presence or absence of characteristic peaks in the biochemical fingerprints of Gastrodia elata. According to the biochemical fingerprint characteristics of the above nine species of Gastrodia elata, they can be divided into three categories:

[0055] Class I includes Gastrodia elata standard products, Shimen in Hunan, Yichang in Hubei, Dabie Mountain in Anhui, Dafang in Guizhou, Lueyang in Shaanxi, Chayutian in Tibet, and Gastrodia elata in Changbai Mountain in Jilin. The biochemical fingerprint characteristics of this group of Gastrodia elata are: the separation peak of gastrodin is higher than the separation peaks of all other components.

[0056] Class II includes Yunnan Yiliang Gastrodia elata. The fingerprint ch...

Embodiment 3

[0059] Embodiment 3, the present invention is used for the identification and evaluation of Gastrodia elata quality

[0060] According to the active ingredient gastrodin peak area of ​​Gastrodia elata samples, the formula of the present invention is used to calculate the average content of gastrodin in various Gastrodia elata samples (see Table 1).

[0061] The average gastrodin content of the 19 kinds of Gastrodia elata samples and the average peak area sum of all biochemical components

[0062]

[0063] *HY=Hubei Yichang Gastrodia, HS=Hunan Shimen Gastrodia, SHL=Shaanxi Lueyang, GD=Guizhou Dafang Gastrodia, AD=Anhui Dabie Mountain Gastrodia, XC=Tibet Chayu Gastrodia, SL=Sichuan Liangshan Gastrodia, JC=Jilin Changbaishan Gastrodia, YY = Yunnan Yiliang Gastrodia elata.

[0064] Gastrodia elata samples from Yichang, Hubei and Shimen, Hunan contain the highest gastrodin, reaching 7.519mg / g and 7.225mg / g respectively, and the contents of other components are also high (calcul...

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Abstract

The invention discloses technology for classifying biochemical fingerprint spectrums of Gastrodia elata Blume and identifying quality of the Gastrodia elata Blume and application thereof, and relates to Chinese medicament and natural medicament identification and quality evaluation technology. The invention provides the following key technical parameters for separating and detecting biochemical components of the Gastrodia elata Blume: the optimum mobile phase (acetonitrile solution with gradient concentration), the optimum detection wavelength (219nm) and other basic conditions, and provides the technology for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume and a formula and a method for calculating the content of an effective component gastrodin in the Gastrodia elata Blume. The technology can be used for detecting the biochemical fingerprint spectrums of the Gastrodia elata Blume, classifying varieties of the Gastrodia elata Blume and identifying and evaluating the quality of the Gastrodia elata Blume, and also can be used for determining regions suitable for growing the Gastrodia elata Blume and identifying the truth of the Gastrodia elata Blume.

Description

technical field [0001] The invention relates to identification and quality evaluation technology of traditional Chinese medicine and natural medicine, in particular to biochemical fingerprint classification, quality identification and evaluation technology of gastrodia elata and its application. Background technique [0002] Among all quality control systems, biochemical fingerprinting technology is recognized as the most effective scientific method for identifying and evaluating the quality of traditional Chinese medicines and natural medicines. The main feature of the biochemical fingerprint is that it can not only show the commonality of the same medicine from different sources, but also show the particularity of the natural medicine of different genetic groups of the same kind, and can quantitatively analyze and evaluate certain components of the medicine according to the peak area . Among all the methods for constructing biochemical fingerprints, high-performance liqui...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/36G01N30/86A61K36/8988A61K125/00
Inventor 陶钧陈平
Owner 西藏和藤医药发展股份有限公司
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