Method for identifying soybean seed authenticity and/or purity of variety
A variety purity, soybean seed technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, material electrochemical variables, etc., can solve the difficult identification of seed authenticity and variety purity, complex procedures, technical content and detection Problems such as high cost, to achieve the effects of difficult storage and transportation, simplified experimental steps, omitting DNA purification and RNA degradation
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[0077] Example 1. Using Jinda 75 seeds as standard seeds to identify the authenticity and variety purity of soybean seeds
[0078] Before identification, more than 20 soybean varieties from Shanxi Province (including Jinda 75) were screened for SSR markers, and it was found that SSR primer Sat_344 can produce specific bands on the genomic DNA of Jinda 75. Therefore, primers Sat_344 were used. The DNA of Jinda 75 samples for inspection was analyzed.
[0079] 1. Seed sample selection
[0080] A random number of 95 seeds were selected from the soybean variety Jinda 75. To test the identification effect, 3 seeds of Jinda 74 and 2 seeds of Jinda 701 were added. A total of 100 seeds were used as test samples.
[0081] 2. DNA extraction from seed samples
[0082] (1) Use tongs to crush the soybean seeds one by one, and place each seed in a 1.5ml centrifuge tube. The sample occupies about 1 / 3 of the volume of the centrifuge tube.
[0083] (2) Add 600μl CTAB buffer to each 1.5ml centrifuge...
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