Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling

A technology for aseptic seedlings and wolfberry, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high lethality, high seed toxicity, reduced germination rate of wolfberry, etc., shortening cycle and improving sterility rate. Effect

Inactive Publication Date: 2014-03-05
SHANDONG CROP GERMPLASM CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

If the explants are taken from the natural environment, generally the explants should be aseptically treated before callus induction, and aseptic treatment generally uses 0.1% mercury chloride, which is highly toxic to the explants. The damage is greater and the lethal rate is higher, resulting in a lower callus induction rate; in addition, the aseptic Lycium barbarum seedlings can be directly subjected to physical trauma trea

Method used

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  • Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling
  • Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling
  • Medlar sterile seedling and method for obtaining induced healing of medlar sterile seedling

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Example Embodiment

[0025] Example 1

[0026] (1) Fruit drying: Put the collected wolfberry fruit in a nylon mesh bag, hang it in a dry and ventilated room, and place it at 20-25℃ for about 14 days, turning it every other day until the fruit is completely dry ;

[0027] (2) Seed disinfection: When the seeds and the peel are easy to separate, remove the seeds, place them in a sterile blue-cap screw-top reagent bottle under aseptic conditions, add the treatment solution, and place the blue-cap screw-top reagent bottle on a shaking shaker , Under the condition of 20-25℃, shake quickly for 2 minutes, then pour the seeds and the treatment solution together on dry sterile filter paper, and dry treatment under aseptic conditions; the treatment solution contains 70% (v / v ) Ethanol, 0.1% (v / v) Triton X-100 polyethylene glycol octyl phenyl ether) sterile distilled water, the resistivity of the distilled water is ≥0.1×10 6 Ω·cm (Ou·cm).

[0028] The treatment solution in step (2) of Example 1 was changed to ...

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Abstract

The invention discloses a medlar sterile seedling and a method for obtaining the induced healing of the medlar sterile seedling, belonging to the technical field of plant tissue culture. The method comprises the following steps of drying fruits; disinfecting seeds: carrying out the seed disinfection by using sterile distilled water which contains 70 percent by weight of ethanol and 0.1 percent by weight of TritonX-100 (polyethylene glycol octylphenol ether); germinating seeds: firstly arranging the seeds into a refrigerator at 4 DEG C, standing for 2 days, and then removing the seeds to a culture room at the temperature of 25+/-2 DEG C with the daily photoperiod of 14 hours to culture for 20+/-2 days; healing subculture: arranging the seed on a subculture medium, culturing under at 25+/-2 DEG C in the culture room with the daily photoperiod of 16 hours, and replacing the culture medium every two weeks. According to the method disclosed by the invention, both the medlar sterile seedling and the medlar induced callus are obtained, the step of carrying out physical trauma on the medlar callus in the inducing process of the medlar callus is simplified and the period of obtaining the medlar callus is shortened.

Description

technical field [0001] The invention relates to a method for tissue culture callus induction, in particular to a method for obtaining a sterile Lycium barbarum seedling and induced callus. Background technique [0002] Lycium is a multi-branched, thorny shrub. Its fruit has many folk names in China, such as Gouqizi, Lycium Hongshi, Beet Seed, Western Lycium, and Gou Naizi. Lycium barbarum is listed by the Ministry of Health as a variety of "dual-use medicine and food". Lycium barbarum can be processed into various foods, beverages, health wine, health care products, etc. The main components of Lycium barbarum contain betaine, atropine, gyoscyamine, Lycium barbarum polysaccharide and Lycium barbarum pigment. Among them, the immune regulation and anti-tumor effects of Lycium barbarum polysaccharides are the most studied. Many studies have shown that wolfberry has the functions of promoting immunity, anti-aging, anti-tumor, preventing atherosclerosis, scavenging free radicals...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 丁汉凤王俊峰李润芳李娜娜余华贾文斌
Owner SHANDONG CROP GERMPLASM CENT
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