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Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles

A technology of microfluidic chips and magnetic particles, which is applied in measuring devices, scientific instruments, instruments, etc., can solve the problem that the detection method is not convenient enough, the non-quantitative gold standard test strips are not suitable for on-site detection, bedside detection, and gold standard test strips Insufficient quantitative detection and other problems, to avoid perfusion sample injection operations, reduce edge effects, and avoid sample reflux

Inactive Publication Date: 2015-11-11
INST OF ELECTRONICS CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The present invention aims at the rapid, sensitive and convenient detection requirements of low-concentration substances in trace samples such as blood samples, in order to solve the inconvenient and non-quantitative limitations of existing detection methods: 1) the current detection is aimed at blood samples CRP and its lower concentrations of protein substances and cells require multi-step operations, which are not suitable for rapid on-site detection, bedside detection, etc.; 2) multi-step detection reactions increase errors in each step of operation, micropumps The difference in sample injection control will lead to the deviation of the test results and increase the complexity of the test; 3) The quantitative test of the gold standard test strip is insufficient

Method used

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  • Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles
  • Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles
  • Method of detecting pathogeny based on optical microfluidic chip of magnetic microparticles

Examples

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Embodiment 1

[0058] figure 1 A schematic structural view of a one-step detection optical microfluidic chip based on magnetic particles is shown according to the first embodiment of the present invention. Such as figure 1 As shown, the optical microfluidic chip includes a lower substrate, an upper cover plate, a calibration tank, a dilution tank, a sampling tank, a channel, a calibration tank, a reaction tank, an exhaust hole, a press valve, a liquid storage bag, and an awl needle. The microfluidic immune chip is used for rapid and sensitive detection of extremely low concentration substances such as protein macromolecules in blood samples, urine samples, saliva and other biological samples.

[0059] Wherein, the microfluidic immune chip based on magnetic particles is an integrated chip prepared based on MEMS technology, magnetic particle enrichment and separation technology, immune technology and F-TIR optical detection principle.

[0060] Among them, the upper cover plate adopts PDMS pr...

Embodiment 2

[0066] figure 2 A schematic structural view of a one-step detection optical microfluidic chip based on magnetic particles is shown according to the second embodiment of the present invention. Such as figure 2 As shown, the optical microfluidic chip includes a lower substrate, an upper cover plate, a calibration tank, a sample loading tank, a channel, a calibration pool, a reaction pool, an exhaust hole, a press valve, a liquid storage bag and an awl needle. Compared with Example 1, the optical microfluidic chip in this example does not have a dilution tank.

[0067] Both the lower base plate and the upper cover plate are made of transparent plastic plates, which are prepared by hot pressing molding technology, and at the same time, calibration grooves, sample filling grooves, sample feeding vents, and calibration vents are formed on the upper cover plate.

[0068] Channels, calibration pools, and reaction pools are prepared with ultra-thin double-sided adhesive tape, and t...

Embodiment 3

[0072] image 3 It is a schematic structural diagram of a one-step detection optical microfluidic chip based on magnetic particles according to the third embodiment of the present invention, as shown in image 3 As shown, the optical microfluidic chip includes: a lower substrate, an upper cover plate, a liquid storage tank, a sample loading tank, a channel, a reaction pool, a comparison pool, an exhaust hole, a press valve, a liquid storage bag and an awl needle.

[0073] The lower substrate is made of transparent plastic plate, which is prepared by hot-press forming technology. The upper cover plate is made of hydrophilic single-sided adhesive material, and laser engraving technology is used to form liquid storage tanks, sample adding tanks, channels, and reaction channels on the hydrophilic single-sided adhesive material. Pools, contrast pools and vents.

[0074] The channel, contrast pool and reaction pool are prepared with ultra-thin double-sided tape, and the graphics of...

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Abstract

The invention discloses a method of detecting pathogeny based on an optical microfluidic chip of magnetic microparticles, wherein the optical microfluidic chip of the magnetic microparticles is provided with at least a reaction tank; a prefixed antibody, namely the magnetic microparticles are arranged at the inner side of a top cap of the reaction tank; a prefixed and paired specific recognition monoclonal antibody or polyclonal antibody is arranged at the bottom side of the reaction tank through imprinting by a tamp or through micro-nano sample application; the method comprises the following steps: the determinand in a sample to be detected and the antibody, namely the nanometer magnetic microparticles in the reaction tank are performed a conjugation reaction; the antibody, namely the nanometer magnetic microparticles move up and down in the reaction tank under the action of further changing a magnetic field, and the contact between the antibody, namely the nanometer magnetic microparticles and the determinand in the sample to be detected is increased, thus the speed of reaction and combination is improved, and the reaction time is reduced. Extra magnetic microparticles are further adsorbed to the inner side of the top cap of the reaction tank through changing the direction of the magnetic field; the magnetic microparticles combined with the determinand are fixed at the bottom side of the reaction tank through the action of combining of the paired antibody; the concentration of the determinand is proportional to the density of the nano magnetic microparticles fixed at the bottom side of the reaction tank finally.

Description

technical field [0001] The invention relates to the technical field of biosensor detection, and is a method for detecting pathogens using an optical microfluidic chip based on magnetic microparticles using microfluidic technology, immune technology and nanomagnetic particle technology. Background technique [0002] The development of point-of-care testing (POCT) products has experienced the first generation of qualitative testing using test strips, the second generation of semi-quantitative testing using color charts or semi-quantitative instrument reading, and the second generation of semi-quantitative testing using manual operations. The third-generation full quantitative system, and the fourth-generation technology platform that adopts automation, information and intelligence. A microfluidics chip is a microchip that can complete a biochemical analyzer. It can realize the miniaturization of the original testing instrument and make it a portable instrument for bedside test...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543
CPCG01N33/54326
Inventor 刘春秀刘昶蔡浩原贾建
Owner INST OF ELECTRONICS CHINESE ACAD OF SCI
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