A kind of extraction method of fragrant pear protein

An extraction method and protein technology, which is applied in the field of natural product extraction, can solve the problems of blurred protein spots, horizontal and vertical stripes tailing, and low resolution in two-dimensional electrophoresis, and achieve the effect of clean gel background, uniform distribution, and more protein spots

Active Publication Date: 2020-06-05
SHIHEZI UNIVERSITY
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the TCA-acetone method is often used to extract the protein in fragrant pears, but the protein spots in the two-dimensional electrophoresis map obtained from the protein are blurred, the resolution is low, and the problems of horizontal and vertical stripes and tailing are serious. After statistical analysis by PDQuest 8.0.1 software, in the same Under the parameter setting conditions, the TCA / acetone precipitation method can distinguish 126 protein spots on the gel surface, and the obtained protein spots are very few

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of extraction method of fragrant pear protein
  • A kind of extraction method of fragrant pear protein
  • A kind of extraction method of fragrant pear protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Before grinding, pour liquid nitrogen into the mortar to pre-cool, then chop 1g of fragrant pear pulp until it has no obvious lumps, put it in the mortar, add liquid nitrogen quickly, and grind until the tissue becomes powder, uniform without obvious lumps. Granules; Immediately add 100mg crospovidone into the mortar, then transfer to a 50mL centrifuge tube with a medicine spoon;

[0070] Add 10mL extract to a 50mL centrifuge tube containing pear pulp powder, then add 10mL Tris saturated phenol, shake it manually for 5 minutes, place it on ice, shake once every 5 minutes, a total of 6 times; Centrifuge for 30 minutes, suck out the first phenolic phase, and transfer it to a 15mL centrifuge tube; the extract uses water as a solvent and includes the following components: 0.7mol / L sucrose, 0.1mol / L KCl, 0.05mol / L EDTA, 0.5mol / L L Tris-HCl; the pH value of the extract is 7.5.

[0071] Add the same volume of extract as the first phenolic phase phenol into a 15mL centrifuge t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention provides an extraction method of bergamot pear proteins. The extraction method comprises the following steps: grinding bergamot pear pulp in a liquid nitrogen atmosphere to obtain a grinded object; mixing the grinded object with polyvinylpolypyrrolidone to obtain a mixture containing the polyvinylpolypyrrolidone; mixing the mixture containing the polyvinylpolypyrrolidone with an extraction liquid and Tris saturated phenol, performing vibration after mixing, putting the vibrated material on ice to insulate, and centrifugalizing the insulated slurry to obtain a first phenol phase;mixing the first phenol phase with the extraction liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate and centrifugalizing the insulated slurry to obtain a second phenol phase; and mixing the obtained second phenol phase with an ammonium acetate methanol liquid, vibrating the mixture, putting the vibrated slurry on ice to insulate, and leaving the insulated slurry overnight at -10-30 DEG C to obtain the bergamot pear proteins. By adopting the extraction method provided by the invention, the quantity of differentiating protein points can be increased.

Description

technical field [0001] The invention relates to the technical field of natural product extraction, in particular to a method for extracting fragrant pear protein. Background technique [0002] Korla Fragrant Pear belongs to Xinjiang pear species. It is originally produced in Bayingoleng Mongolia Autonomous Prefecture, Aksu and other places in southern Xinjiang. It has a cultivation history of 1,300 years and is an ancient local excellent variety. Korla fragrant pear has thin skin, crisp texture, white flesh, tender flesh, juicy and sweet taste, slightly sour near the core, and strong fragrance. The planting area in Korla City, Bazhou is 24,000 mu, and the annual output is more than 1,000 tons. , exported to the United States, Southeast Asia, Hong Kong, Macao and other countries and regions. [0003] However, the occurrence of pear blackhead has seriously affected the fruit quality and commodity value of pears. Pear blackhead is a latent infectious disease. According to the ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C07K1/14C07K1/30
CPCC07K1/145C07K1/30
Inventor 郭敏瑞程少波陈国刚张伟达陈诺
Owner SHIHEZI UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap