Molecule detection method of Fusarium graminearum to medium resistance level bacterial strain of carbendazim

A technology of molecular detection of Fusarium graminearum, applied in the field of epidemic prediction of drug-resistant wheat head blight, can solve problems such as failure of chemical control, achieve the effects of controlling drug resistance, reducing environmental pollution, and reducing costs
CN101985653AInactive Publication Date: 2011-03-16NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2011-03-16
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to a molecule detection method of Fusarium graminearum to medium resistance level bacterial strain of carbendazim. The method can be used for monitoring drug resistance of Fusarium graminearum to carbendazim and prevalence warning, wherein Fusarium graminearum can cause wheat scab. The detection method includes the following three main steps: (1) nuclear genome DNA of bacterial strain to be detected is respectively extracted: (2) internal and external primer pairs are utilized to carry out nested PCR, thus obtaining a target segment; (3) the target segment is respectively restricted by restriction enzymes HindIII and TaaI (Tsp4CI), and genotype of medium resistance level bacterial strain can be accurately identified by virtue of a restriction product electrophoresis spectrum. By adopting PIRA-PCR (primer-introduced restriction analysis PCR) technology, quantity of Fusarium graminearum with medium drug resistance in field and ratio thereof in group can be rapidly and accurately detected. Detection accuracy reaches more than 95%.
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Description

1. Technical field

[0001] The invention belongs to a molecular detection method for strains of Fusarium graminearum with a medium level of resistance to carbendazim, which can be used for monitoring the development trend of the benzimidazole fungicide (carbendazim)-resistant Fusarium graminearum population, Prediction of the prevalence of insecticide-resistant wheat head blight. 2. Technical background

[0002] Wheat scab is a worldwide disease caused by Fusarium graminearum Schwabe, which seriously affects the yield and quality of wheat. For a long time, benzimidazole fungicides or compound agents based on such agents have been used for chemical control. Benzimidazole fungicides include carbendazim, benomyl, thiabendazole, thiophanate, etc. As a class of high-efficiency, broad-spectrum systemic fungicides used in production, it solves the problem of environmental toxicity of protective fungicides and improves the ability of humans to control the disease. Benzimidazole fu...

Claims

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