Microfluidic chip for detecting heavy metal ions in water and detection method

Abstract

The invention belongs to the field of environmental protection, and particularly relates to a microfluidic chip for detecting heavy metal ions in water and a detection method. The microfluidic chip comprises micro-channels and micro-valves, wherein the micro-channels comprise a reference micro-channel, an ion imprint micro-channel, an electrophoresis sample introduction micro-channel, an electrophoresis separation micro-channel, a connection micro-channel, a waste liquor exhaustion micro-channel and a buffer solution exhaustion micro-channel; the micro-valves comprise a reference end outlet micro-valve, an ion imprint exit micro-valve, a waste liquor exit micro-valve, a buffer solution cut-off micro-valve and a sampling cut-off micro-valve. The detection method comprises the following steps: acquiring a first electropherogram of cross electrophoresis before heavy metal ion adsorption; then, acquiring a second electropherogram of cross electrophoresis after heavy metal ion adsorption; finally, recognizing specific heavy metal ions according to the difference between the two electropherograms, and calculating heavy metal contents. The microfluidic chip and the detection method have the advantages that not only are the production cost and the use cost very low, but also the anti-interference ability is very high; moreover, the detection process is simple, and the detection time is short.

Description

technical field [0001] The invention belongs to the field of environmental protection, and in particular relates to a microfluidic chip and method for detecting heavy metal ions in water. Background technique [0002] Heavy metal ions in water will not only damage the ecological environment, but also threaten human health. Therefore, it is necessary to detect heavy metal ions in water. Traditional heavy metal ion detection equipment includes detection equipment based on colorimetry, detection equipment based on atomic absorption spectroscopy, and detection equipment based on biosensing, etc. These traditional equipment have played an important role in the field of heavy metal ion detection. However, in During the process of use, it was found that these traditional devices also had the following problems: [0003] First, because the traditional detection equipment is jointly completed by multiple components, the integration degree is low, so these detection equipment have la...

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Problems solved by technology

[0003] First, because the traditional detection equipment is jointly completed by multiple components, the integration degree is low, so these detection equipment have large volume, high quality, and high power consumption. The three "big" problems increase the production cost and use cost of the equipment;

[0004] Second, because some traditional detection equipment only focus on the heavy metal ions to be detected, and lack the function settings to shield other interferences, these detection equipment cannot detect the target heavy metal in the presence of multiple heavy metal ions or other pollutants. The identification of ions will be reduced, affecting the detection accuracy;

[0005] Third, because some traditional detection equipment needs a pretreatment process before detecting heavy metal ions, the detection process is relatively complicated and the detection time is long;

[0006] Fourth, due to the toxicity of the reagents used in the traditional method,

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