6 results about "Actin genes" patented technology

The present invention relates to the fields of biotechnology and aquaculture, and in particular to a method for increasing the growth rate of fishes. The method comprises the following steps: fusing a coding region of a gh1 gene with a strong promoter of another endogenous highly-expressed gene in vivo, without introducing an exogenous gene, to generate a gh1 highly-expressed gene, wherein the strong promoter is a β-actin gene promoter. The present invention can create fast-growing fish species.

This invention discloses a rapid detection method for African swine fever virus (ASFV), belonging to the field of animal disease detection. The method specifically includes the following steps: S1, preparation of propidium azidobromide working solution; S2, preparation of diluted virus solution; S3, virus treatment with propidium azidobromide; S4, qPCR primer and probe design; S5, extraction and purification of the virus solution after dark and light treatment using a DNA extraction kit. After completion, primers and probes based on the porcine actin gene β-Actin are designed, and a qPCR reaction system is constructed for qPCR analysis. This invention solves the problem of low detection sensitivity and false positives in existing technologies due to the inability of PMA to effectively bind to the DNA of dead virus caused by capsid aggregation after viral death. It has the potential application in detecting ASFV levels in pig feed, the environment, and live pigs.

The invention belongs to the field of biotechnology and aquaculture, and particularly relates to a method for increasing the growth speed of fishes. The method comprises the following steps that under the condition that an exogenous gene is not introduced, a coding region of a gh1 gene and a strong promoter of another endogenous high-expression gene are fused in vivo, the gh1 high-expression gene is generated, and the strong promoter is a beta-actin gene promoter. Fish varieties which grow rapidly can be created.

The invention discloses application of quercetin in preparation of drugs for preventing and / or treating ALT tumors, and relates to the technical field of biological medicines. The research finds that quercetin can inhibit the activity and proliferation of ALT tumor cells, increase the DNA damage level and replication pressure at the telomeres of the ALT tumor cells and inhibit the expression of MRTF-A genes or Actin genes, thereby achieving the purpose of preventing and / or treating ALT tumors. Experiments prove that quercetin can inhibit proliferation of ALT tumor cells in vivo and in vitro, and the main action target is an MRTF-A gene, an Actin gene or an Actin protein. Therefore, the MRTF-A gene, the Actin gene or the Actin protein can be used as a new target spot for ALT tumor treatment, and the quercetin and Actin protein / Actin gene inhibitor has great application prospects in preparation of drugs for preventing and / or treating ALT tumors.

The present application relates to the field of aquatic animal disease-resistant breeding, and discloses a method for multi-index combined detection of the anti-DIV1 virus ability of Macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof, which comprises the following steps: after the Macrobrachium rosenbergii is anesthetized and disinfected, abdominal limb samples are obtained by minimally invasive cutting of abdominal limbs, and the live body is placed in water for recovery; total RNA is extracted and reversely transcribed into a cDNA template; specific primers are used for fluorescence quantitative PCR detection of chitinase 1C gene, C-type lectin domain-containing protein gene, hemocyanin gene, actin gene and an internal reference gene; and the relative expression data are standardized, directionally corrected, weighted and converted to obtain a comprehensive disease resistance index and a determination result. The present application obtains samples by minimally invasive cutting of abdominal limbs, ensures the survival and recovery of the live body, facilitates the in-vivo breeding of excellent individuals, and improves the accuracy and stability of detection and evaluation by combining multi-dimensional target genes for joint detection.

The application provides a primer probe and kit for detecting SMN1 gene mutation. The kit comprises primers for detecting deletion mutation of SMN1 gene exons 7 and 8 respectively, and corresponding mutation detection probes and blocking probes. The application is more accurate and reliable by reducing the interference of SMN2 pseudogene. The triple amplification of SMN1 gene exons 7 and 8 and the reference Actin gene is completed in a single reaction tube by using the principle of multiplex PCR amplification, thereby improving the efficiency. Through reaction system optimization, the micro blood sample can be used for PCR amplification without purification of genomic DNA, the pretreatment is completed in 15 minutes, the whole detection is completed in about 2.5 hours, time and purification cost are saved, and the kit is suitable for high-throughput screening and detection of neonatal dried blood spots.