21 results about "Actin genes" patented technology

The invention discloses dsRNA / fused dsRNA for RNAi targeting multiple aphids and application of the dsRNA / fused dsRNA, and the dsRNA targets an aphid Actin gene and / or an ELAV-like protein gene and is obtained by transcription by taking the aphid Actin gene and / or the ELAV-like protein gene as a template. Compared with the traditional design thought that dsRNA of pests mostly aims at one pest and is targeted to a single gene at present, the strategy is changed, starting from the thought that one dsRNA can prevent and treat various aphids, highly conservative genes of various aphids are found, the dsRNA is designed for the highly conservative genes, and it is found that in a plurality of candidate genes, various aphids can be effectively prevented and treated. The dsRNA aiming at the actin Actin gene and the ELAV-like protein gene has a remarkable fatality rate on various aphids and has a very good application prospect, the effect of simultaneously targeting the fused dsRNA of the two genes is further improved, and the dsRNA has a remarkable insecticidal effect on various aphids.

The invention relates to dsRNA capable of simultaneously preventing and treating various thrips and application of the dsRNA. Specifically, the invention provides a dsRNA construct, the construct of the dsRNA is a double strand, and the construct of the dsRNA comprises a nucleotide sequence of an insect nymph and / or adult stage regulation related gene or fragment; wherein the insect nymph and / or adult stage regulation related gene is an Actin gene, and the dsRNA is obtained by amplifying a sequence as shown in SEQ ID NO: 9-10. The fusion type dsRNA is designed to target a plurality of conservative thysanoptera actin gene dsRNA, so that the multi-killing effect is achieved, and the prevention and treatment effect of the fusion type dsRNA is equivalent to that of spinetoram which is commonly used in the market at present.

The invention discloses a detection method for detecting enteropathogenic bacteria, and relates to the field of molecular biology, specific genes are searched in genomes of salmonella, shigella, vibrio parahaemolyticus, campylobacter jejuni, diarrheagenic escherichia coli, vibrio cholerae and staphylococcus aureus, a beta-actin gene is adopted as a reference gene, and the enteropathogenic bacteria are detected. Primer and probe sequences are designed, a probe group consisting of the primer and probe sequences is used for carrying out real-time fluorescent PCR amplification reaction on nucleic acid in a detected sample, then melting curve analysis is carried out, and whether the pathogenic bacteria exist in the nucleic acid sequence to be detected or not can be judged according to a Tm value. The detection method provided by the invention has the advantages of rapidness, high throughput, accuracy and high sensitivity.

The invention discloses a molecular marker for high-throughput identification of a wheat scab site Qfhb7EL and application of the molecular marker. The nucleotide sequences of primers of the molecular marker are as shown in SEQ ID No. 1 to SEQ ID No. 4. Specific primers are designed according to the homologous sequence of the Fhb7 gene in the triticum elongatum, the specific primers and the Actin gene of wheat form a primer pair, population typing is achieved under recognition of different fluorescence, and the reliability of the marker is verified through molecular experiments. Results show that the identification of the wheat scab-resistant site Qfhb7EL in the wheat background can be quickly and accurately realized in a high-throughput manner, and the method has a very high application value for auxiliary selection of new wheat scab-resistant varieties.

The present invention relates to the fields of biotechnology and aquaculture, and in particular to a method for increasing the growth rate of fishes. The method comprises the following steps: fusing a coding region of a gh1 gene with a strong promoter of another endogenous highly-expressed gene in vivo, without introducing an exogenous gene, to generate a gh1 highly-expressed gene, wherein the strong promoter is a β-actin gene promoter. The present invention can create fast-growing fish species.

The present invention discloses a primer and probe combination and a kit for detecting galactose-1-phosphate uridyltransferase gene polymorphism, which relates to the technical field of in vitro detection. The primer and probe combination for detecting galactose-1-phosphate uridyltransferase is detected in two categories, and each category of primer and probe group has 3 primer and probe combinations, which can simultaneously detect 8 mutation sites of the galactose-1-phosphate uridyltransferase gene, shortening the diagnosis time and reducing the detection cost; Taqman probes and MGB probes are used in the same category of primer and probe group, which can better distinguish the Tm values of heterozygous samples, reduce the problems of missed detection or genotype misjudgment, and improve the detection sensitivity and accuracy; In addition, the β-actin gene internal reference is used for quality control, improving the comparability of the detection.

The invention provides a primer and a probe for detecting related pathogens of vaginitis through multiple RPA (recombinase polymerase amplification)-lateral flow immunochromatography. The primer comprises a neisseria gonorrhoeae porA gene primer pair, a gardnerella vaginalis 16S rRNA (ribosomal Ribonucleic Acid) gene primer pair, a candida albicans ITS2 gene primer pair, a trichomonas vaginalis actin gene primer pair and a ureaplasma urealyticum Urease gene primer pair. The probes comprise a gold nanoparticle binding probe, a quality control line capture probe and capture probes corresponding to the five genes. The invention also provides application of the primer and the probe in preparation of vaginitis-related pathogen detection products, and also provides a corresponding detection kit and method. According to the invention, the rapid and accurate detection of five common vaginitis pathogens is realized through the RPA-lateral flow immunochromatography technology, and the kit is suitable for rapid, early and convenient diagnosis of vaginitis patients.

The invention relates to an ONT sequencing library quantitative kit and method, the ONT sequencing library quantitative kit comprises a primer group and a TaqMan probe, the primer group is used for specifically amplifying a beta-actin gene, the amplification area of the primer group covers at least partial area of a fifth exon and at least partial area of a sixth exon of the beta-actin gene, and the TaqMan probe is used for specifically amplifying the beta-actin gene. And the TaqMan probe can be specifically combined with an amplification region of the primer group. The application finds that the concentration of the ONT sequencing library and the qPCR amplification signal of the specific region of the beta-actin gene have a linear relationship, the ONT sequencing library can be quantitatively detected, absolute quantification of the ONT sequencing library can be realized, the actual concentration of the ONT sequencing library can be more accurately obtained, correction of the size of a DNA fragment based on the ONT sequencing library does not need to be carried out, and the detection efficiency is greatly improved. The sequencing quality can be improved, so that an optimal sequencing data result is obtained.

This invention discloses a rapid detection method for African swine fever virus (ASFV), belonging to the field of animal disease detection. The method specifically includes the following steps: S1, preparation of propidium azidobromide working solution; S2, preparation of diluted virus solution; S3, virus treatment with propidium azidobromide; S4, qPCR primer and probe design; S5, extraction and purification of the virus solution after dark and light treatment using a DNA extraction kit. After completion, primers and probes based on the porcine actin gene β-Actin are designed, and a qPCR reaction system is constructed for qPCR analysis. This invention solves the problem of low detection sensitivity and false positives in existing technologies due to the inability of PMA to effectively bind to the DNA of dead virus caused by capsid aggregation after viral death. It has the potential application in detecting ASFV levels in pig feed, the environment, and live pigs.

The invention belongs to the technical field of biology, and particularly discloses a dsSAM50 / dsActin-coated MSNs nano-complex, a preparation method thereof and application of the dsSAM50 / dsActin-coated MSNs nano-complex in prevention and control of large thrips of cowpeas, dsSAM50 and dsActin are simultaneously loaded by adopting nano-carrier mesoporous silica nanoparticles (MSNs), double genes (dsSAM50 / dsActin) are loaded through the mesoporous silica nanoparticles (MSNs), and the dsSAM50 / dsActin-coated MSNs nano-complex is obtained through the preparation method of the dsSAM50 / dsActin-coated MSNs nano-complex and the preparation method of the dsSAM50 / dsActin-coated MSNs nano-complex. The technical bottlenecks that bare dsRNA is easy to degrade and low in delivery efficiency in the traditional RNAi technology are broken through. The complex significantly improves the cell delivery efficiency of lethal genes by virtue of the high load capacity and targeted release characteristic of the nano-material, not only can significantly inhibit the expression of SAM50 and Actin genes of the megalurothrips megalurothrips, but also can significantly reduce the fecundity of the megalurothrips megalurothrips, significantly improves the death rate of the megalurothrips megalurothrips, and has good application prospects. After the two dsRNAs and the MSNs form a complex, the control effect on the cowpea large thrips can be improved.

The present invention discloses a safe transgenic site of the citrus fruit fly genome and a screening method and application thereof. The present invention develops an Actin gene promoter, and further constructs two different citrus fruit fly strains from it, and finds a feasible safe transgenic insertion site of the citrus fruit fly genome, thereby providing important technical support for the strain development of the citrus fruit fly and the prevention and control work with gene editing as the core.

The invention discloses a primer and probe combination and a kit for detecting gene polymorphism of galactose-1-phosphate uridinetransferase, and relates to the technical field of in vitro detection.The primer and probe combination for detecting galactose-1-phosphate uridinetransferase is divided into two types for detection, each type of primer and probe combination comprises three types of primer and probe combinations, and each type of primer and probe combination comprises three types of primers and probes. Mutation sites of eight galactose-1-phosphate uridine transferase genes can be detected at the same time, the diagnosis time is shortened, and the detection cost is reduced; a taqman probe and an MGB probe are used in the same type of primer and probe group, so that the Tm value of a heterozygous sample can be better distinguished, the problems of missing detection or genotype misjudgment are reduced, and the detection sensitivity and accuracy are improved; in addition, the beta-actin gene internal reference is used for quality control, so that the comparability of detection is improved.

The invention discloses a pActin:: AcFT1 fusion gene for regulating and controlling early flowering of kiwi fruits, a method and application. The nucleotide sequence of the pActin:: AcFT1 fusion gene is shown as SEQ ID NO. 3. According to the invention, an expression vector containing a pActin:: AcFT1 fusion gene is constructed by cloning a promoter sequence of a kiwi fruit Actin gene and a coding sequence of an AcFT1 gene, and a transgenic kiwi fruit plant of pActin:: AcFT1 is obtained by using an agrobacterium-mediated leaf infection method. Results show that the expression level of AcFT1 in a transgenic plant is obviously higher than that of a wild type; after the transgenic plant is transplanted to a soil matrix, the transgenic plant shows a phenotype of early flowering. The method is helpful for shortening the breeding period of the kiwi fruits, and provides theoretical basis and technical support for efficient and rapid breeding of the kiwi fruits.

The invention discloses application of an Actin gene and / or an RPL35 gene in preparation of a reference gene for quantitative analysis of gene expression in a camellia oleifera seed kernel development stage. Candidate genes which are stably expressed in different development stages are screened based on transcriptome data, the expression stability of the candidate genes is evaluated through qRT-PCR in combination with a plurality of stability evaluation methods (geNorm, NormFinder, BestKeeper, Delta-Ct and RefFinder) systems, and finally Actin and RPL35 are determined as optimal reference genes. The invention also provides a specific primer of the reference gene, and the specific primer has the characteristics of high amplification efficiency, strong specificity and the like, is suitable for standardized detection of the expression quantity of the target gene in the camellia oleifera seed kernel development stage, and can effectively improve the accuracy and repeatability of gene expression analysis; and a stable and reliable molecular tool is provided for subsequent grease accumulation and functional gene research.

The invention belongs to the field of biotechnology and aquaculture, and particularly relates to a method for increasing the growth speed of fishes. The method comprises the following steps that under the condition that an exogenous gene is not introduced, a coding region of a gh1 gene and a strong promoter of another endogenous high-expression gene are fused in vivo, the gh1 high-expression gene is generated, and the strong promoter is a beta-actin gene promoter. Fish varieties which grow rapidly can be created.

Described herein various PCR based assays that can detect H. penaei and / or V. parahaemolyticus that causes AHPND in a nucleic acid sample obtained from one or more shrimp. In some aspects, the PCR based assays can detect one or more of the following genes: the flgE gene from H. penaei, the shrimp 18s rRNA gene, the shrimp beta actin gene from shrimp, a bacterial 16S rRNA gene from bacteria (any type of bacteria) the Vibrio pirA gene, and the Vibrio pirB gene. The assays described herein can be single assays or can be multiplexed such that more than one gene and / or more than one bacterial species can be detected in a single reaction. Other compositions, compounds, methods, features, and advantages of the present disclosure will be or become apparent to one having ordinary skill in the art upon examination of the following drawings, detailed description, and examples.

The invention discloses application of quercetin in preparation of drugs for preventing and / or treating ALT tumors, and relates to the technical field of biological medicines. The research finds that quercetin can inhibit the activity and proliferation of ALT tumor cells, increase the DNA damage level and replication pressure at the telomeres of the ALT tumor cells and inhibit the expression of MRTF-A genes or Actin genes, thereby achieving the purpose of preventing and / or treating ALT tumors. Experiments prove that quercetin can inhibit proliferation of ALT tumor cells in vivo and in vitro, and the main action target is an MRTF-A gene, an Actin gene or an Actin protein. Therefore, the MRTF-A gene, the Actin gene or the Actin protein can be used as a new target spot for ALT tumor treatment, and the quercetin and Actin protein / Actin gene inhibitor has great application prospects in preparation of drugs for preventing and / or treating ALT tumors.

The invention relates to the technical field of virus detection, and particularly discloses a dual-identification fluorescent RT-PCR (Reverse Transcription-Polymerase Chain Reaction) detection kit for European and American porcine reproductive and respiratory syndrome viruses. The kit comprises a specific primer pair and a fluorescent probe for targeting a PRRSV European ORF7 gene and an American ORF6 gene, a porcine beta-actin gene endogenous internal standard system, a fluorescent RT-PCR reaction solution and positive and negative controls, wherein the specific primer pair and the fluorescent probe are used for targeting the PRRSV European ORF7 gene and the American ORF6 gene; the kit can efficiently and specifically identify European infection, American infection and mixed infection of PRRSV in the same reaction system, has an endogenous quality control function, effectively monitors sample quality and extraction efficiency, and avoids false negative results; the kit has the characteristics of high detection sensitivity, strong specificity, simplicity and convenience in operation, rapidness and cross contamination resistance, and is suitable for detecting PRRSV nucleic acid in pig tissues, serum, semen and oral swab samples.

The present application relates to the field of aquatic animal disease-resistant breeding, and discloses a method for multi-index combined detection of the anti-DIV1 virus ability of Macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof, which comprises the following steps: after the Macrobrachium rosenbergii is anesthetized and disinfected, abdominal limb samples are obtained by minimally invasive cutting of abdominal limbs, and the live body is placed in water for recovery; total RNA is extracted and reversely transcribed into a cDNA template; specific primers are used for fluorescence quantitative PCR detection of chitinase 1C gene, C-type lectin domain-containing protein gene, hemocyanin gene, actin gene and an internal reference gene; and the relative expression data are standardized, directionally corrected, weighted and converted to obtain a comprehensive disease resistance index and a determination result. The present application obtains samples by minimally invasive cutting of abdominal limbs, ensures the survival and recovery of the live body, facilitates the in-vivo breeding of excellent individuals, and improves the accuracy and stability of detection and evaluation by combining multi-dimensional target genes for joint detection.

The present invention provides methods of treatment, diagnostics, kits, and formulations for treating a subject having one or more genetic variations in an Actin alpha 2 (ACTA2) gene involving administering a therapeutically effective amount of a pterin of Formula I such as sapropterin to a subject, contingent upon a presence of one or more genetic variations in Actin alpha 2 (ACTA2) gene.

The application provides a primer probe and kit for detecting SMN1 gene mutation. The kit comprises primers for detecting deletion mutation of SMN1 gene exons 7 and 8 respectively, and corresponding mutation detection probes and blocking probes. The application is more accurate and reliable by reducing the interference of SMN2 pseudogene. The triple amplification of SMN1 gene exons 7 and 8 and the reference Actin gene is completed in a single reaction tube by using the principle of multiplex PCR amplification, thereby improving the efficiency. Through reaction system optimization, the micro blood sample can be used for PCR amplification without purification of genomic DNA, the pretreatment is completed in 15 minutes, the whole detection is completed in about 2.5 hours, time and purification cost are saved, and the kit is suitable for high-throughput screening and detection of neonatal dried blood spots.