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2 results about "Actin genes" patented technology
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ACTB gene The ACTB gene provides instructions for making a protein called beta (β)-actin, which is part of the actin protein family. The ACTB gene provides instructions for making a protein called beta (β)-actin, which is part of the actin protein family. Proteins in this family are organized into a network of fibers called the actin cytoskeleton, which makes up the structural framework inside cells.
African swine fever virus rapid detection method
This invention discloses a rapid detection method for African swine fever virus (ASFV), belonging to the field of animal disease detection. The method specifically includes the following steps: S1, preparation of propidium azidobromide working solution; S2, preparation of diluted virus solution; S3, virus treatment with propidium azidobromide; S4, qPCR primer and probe design; S5, extraction and purification of the virus solution after dark and light treatment using a DNA extraction kit. After completion, primers and probes based on the porcine actin gene β-Actin are designed, and a qPCR reaction system is constructed for qPCR analysis. This invention solves the problem of low detection sensitivity and false positives in existing technologies due to the inability of PMA to effectively bind to the DNA of dead virus caused by capsid aggregation after viral death. It has the potential application in detecting ASFV levels in pig feed, the environment, and live pigs.
Owner:合肥海关技术中心
A multi-index combined detection method for anti-DIV1 virus ability of macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof
The present application relates to the field of aquatic animal disease-resistant breeding, and discloses a method for multi-index combined detection of the anti-DIV1 virus ability of Macrobrachium rosenbergii based on minimally invasive sampling of abdominal limbs and application thereof, which comprises the following steps: after the Macrobrachium rosenbergii is anesthetized and disinfected, abdominal limb samples are obtained by minimally invasive cutting of abdominal limbs, and the live body is placed in water for recovery; total RNA is extracted and reversely transcribed into a cDNA template; specific primers are used for fluorescence quantitative PCR detection of chitinase 1C gene, C-type lectin domain-containing protein gene, hemocyanin gene, actin gene and an internal reference gene; and the relative expression data are standardized, directionally corrected, weighted and converted to obtain a comprehensive disease resistance index and a determination result. The present application obtains samples by minimally invasive cutting of abdominal limbs, ensures the survival and recovery of the live body, facilitates the in-vivo breeding of excellent individuals, and improves the accuracy and stability of detection and evaluation by combining multi-dimensional target genes for joint detection.
Owner:ZHEJIANG DANSHUI FISHERY RESEARCH INSTITUTE (ZHEJIANG DANSHUI FISHERY ENVIRONMENTAL MONITORING STATION)