Primer for judging bombyx mori moth formation time

Abstract

The invention discloses a primer for judging bombyx mori moth formation time. Actin gene 3 and cocoon lyase gene of bombyx mori chrysalis to be detected are taken as templates, and real-time fluorescent quantitative PCR reaction is performed by using the primer to obtain transcription expression quantity of the actin gene 3 and transcription expression quantity of the cocoon lyase gene separately;the relative transcriptional expression quantity of the cocoon lyase gene is the transcriptional expression quantity of the cocoon lyase gene / the transcriptional expression quantity of the actin gene3; the relative transcriptional expression quantity of the cocoon lyase gene is substituted into a correlation model of the relative transcriptional expression quantity of the bombyx mori chrysalis cocoon lyase gene and pupal stage development time; and the bombyx mori moth formation time is judged. According to the method, the pupal stage development time of one or more bombyx mori chrysalis canbe accurately measured to accurately judge the bombyx mori moth formation time, errors caused by traditional naked eye observation can be reduced, and a new detection method is provided for judging the moth formation time in bombyx mori seed production work.

Description

technical field [0001] The invention belongs to silkworm breeding technology, in particular to a primer for judging the moth moth time of silkworm. Background technique [0002] silkworm( Bombyx mori ) is an economically important insect that has been domesticated in China for more than 5,700 years. Silkworms go through four different developmental stages throughout their life, namely egg, larva, pupa and adult. The metamorphosis and development process from the pupal stage to the adult stage is called eclosion, and this process generally takes 10 to 15 days, and the cocoons are usually picked about 7 days after being put on the cocoon. The silkworm does not need to take in nutrients from the external environment during the pupal stage, but the destruction of larval tissues and organs is violently carried out in the body, and the formation of adult tissues and organs occurs. [0003] In the current production technology, the traditional method is to observe the color chan...

AI Technical Summary

Problems solved by technology

Due to the differences in the color of pupae of different varieties or different batches, it is easy to cause errors only by relying on traditional naked eye observation combined with production experience

It is also reported that the body weight change rate is used to judge the progress of silkworm chrysalis, although it is slightly better than naked eye observation, but it does not have practical application value, because silkworm body weight is greatly affected by individuals

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