Method for fish gene in site modification breeding

An in situ modification, gene technology, applied in the direction of genetic engineering, other methods of inserting foreign genetic materials, plant genetic improvement, etc., can solve the problems that restrict the development, popularization and application of transgenic fish, and the biological safety of transgenic fish affects the effective application, etc. Achieve the effect of eliminating doubts about the safety of GMOs and avoiding transfer
CN1647624AInactive Publication Date: 2005-08-03SUN YAT SEN UNIV +1
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Patent Information

Authority / Receiving Office
CN ยท China
Patent Type
Applications(China)
Current Assignee / Owner
SUN YAT SEN UNIV
Publication Date
2005-08-03
Estimated Expiration
Not applicable ยท inactive patent

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Abstract

The present invention aims at providing one homologous recombination method for in-situ fish gene modification breeding. The method includes the following steps: 1) separating specific fish gene flanking sequence; promoter regulating sequence DNA segments to constitute homologous recombination vector with actin gene promoter regulating sequence to replace growth hormone gene promoter regulating sequence; 2) microscopic injecting the homologous recombination vector to zebra fish embryo to screen positive homologous recombination replaced fish fry; and 3) analyzing relative gene expression level and genetic phynotype of the endogenous gene modified fish. The present invention is used in breeding fish variety.
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Description

technical field

[0001] The invention relates to a method for genetic engineering breeding, in particular to a method for in situ modification and breeding of fish genes. Background technique

[0002] The process of genetic breeding is a process of genome manipulation. Genetic engineering breeding is based on the understanding of gene function and regulatory mechanism, and the genome is modified and transformed at the molecular level, so as to obtain directional genetically improved genetic varieties and get rid of the barriers of genetic fortresses between species. shackles, from passive use of genetic resources to active transformation and innovation. In 1982, Palmiter et al. transferred the rat growth hormone gene to the fertilized mouse eggs and bred fast-growing "super mice". This breakthrough achievement ushered in a new era of transgenic animal research. In 1985, the Institute of Hydrobiology of the Chinese Academy of Sciences took the lead in...

Claims

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