5 results about "Gene dosage" patented technology

The invention relates to a gene dose effect target spot for improving rapamycin resistance and application of the gene dose effect target spot. A target spot gene is YCL039W and / or YCL037C, or human WDR26 and / or LARP1; the YCL039W and / or YCL037C are / is overexpressed in the saccharomycetes, so that the rapamycin resistance of the saccharomycetes can be improved, and the constructed recombinant function can better grow under the pressure condition of the rapamycin; and the sensitivity of the rapamycin can be improved by knocking down the WDR26 and / or the LARP1 in human cells, so that the application of the rapamycin drug resistance in clinical treatment is widened.

The invention provides yeast engineering bacteria for producing L-tryptophan by utilizing methanol carbon source fermentation and application, and belongs to the technical field of biology. According to the engineered yeast, a methanol substrate is coupled with a methanol inducible promoter induction system, so that the gene expression intensity is dynamically matched with the methanol consumption rate, and the problem of regulation lag caused by separation of an inducer and a substrate in a traditional carbon source is solved; in addition, a gene recombination technology is adopted to enhance expression of key genes in the synthesis route of the L-tryptophan, and the biosynthesis efficiency of the L-tryptophan is remarkably improved by increasing the dosage of target genes, enhancing supply of precursor substances and knocking out genes related to catabolism of the tryptophan in chassis cells at the same time. The constructed yeast engineering bacterium has the characteristics of clear metabolic background and strong heterologous expression ability, can be used for synthesizing L-tryptophan from the beginning completely by taking methanol as a carbon source, effectively gets rid of the dependence of a traditional process on grain resources, reduces the production cost, and provides a brand new technical path for industrial production of L-tryptophan.

The invention discloses a construction method and application of a pichia pastoris engineering strain for highly expressing mannase. The heat-resistant mannase derived from thermophilic aerobic bacteria is taken as a research object, and the characteristics of high enzymatic activity, high temperature resistance, wide temperature action range and the like of the heat-resistant mannase are fully exerted. A genetically engineered bacterium capable of stably and efficiently expressing mannase is finally screened out through methods of optimizing a promoter and a signal peptide, increasing the dose of a target gene, co-expressing protein expression related factors and the like, the highest enzyme activity can reach 5635.43 U / ml after 120-hour shake flask fermentation, and a good application prospect is shown.

Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.

The invention relates to a gene dose effect target for improving the tolerance of yeast and application, and particularly provides a group of gene combinations YCL051W-YCL047C capable of enhancing the heat resistance of the yeast, and the gene combinations include YCL051W, YCL050C, YCL049C, YCL048W and YCL047C. A yeast engineering bacterium with heat resistance can be constructed by overexpressing a gene combination YCL051W-YCL047C in a yeast strain, the target combination is suitable for various different types of industrial strains, and the modified strain shows enhanced heat resistance and can accelerate microbial metabolism, improve the production efficiency and remarkably reduce the industrial fermentation cost; meanwhile, the production process efficiency and stability are improved, and the contamination risk is reduced.