11 results about "Gene dosage" patented technology

The invention concerns synthetic miRNAs targeting the TOX gene and uses thereof. Pharmaceutical compositions comprising such miRNAs and uses thereof in the medical field, in particular in the treatment of cancer diseases and immunotherapy, are also described.

The present invention relates to methods, systems, and compositions for counting nucleic acid molecules. The present invention relates to a method for detecting changes in the number of molecules without digital sequencing, in particular for example due to gene duplication or normal euploid complements of chromosomes, compositions and methods, systems, and kits for detecting and quantifying changes in gene dose, such as changes in trisomes of one or more chromosomes typically found in diploid pairs.

The invention relates to a gene dose effect target spot for improving rapamycin resistance and application of the gene dose effect target spot. A target spot gene is YCL039W and / or YCL037C, or human WDR26 and / or LARP1; the YCL039W and / or YCL037C are / is overexpressed in the saccharomycetes, so that the rapamycin resistance of the saccharomycetes can be improved, and the constructed recombinant function can better grow under the pressure condition of the rapamycin; and the sensitivity of the rapamycin can be improved by knocking down the WDR26 and / or the LARP1 in human cells, so that the application of the rapamycin drug resistance in clinical treatment is widened.

The invention provides yeast engineering bacteria for producing L-tryptophan by utilizing methanol carbon source fermentation and application, and belongs to the technical field of biology. According to the engineered yeast, a methanol substrate is coupled with a methanol inducible promoter induction system, so that the gene expression intensity is dynamically matched with the methanol consumption rate, and the problem of regulation lag caused by separation of an inducer and a substrate in a traditional carbon source is solved; in addition, a gene recombination technology is adopted to enhance expression of key genes in the synthesis route of the L-tryptophan, and the biosynthesis efficiency of the L-tryptophan is remarkably improved by increasing the dosage of target genes, enhancing supply of precursor substances and knocking out genes related to catabolism of the tryptophan in chassis cells at the same time. The constructed yeast engineering bacterium has the characteristics of clear metabolic background and strong heterologous expression ability, can be used for synthesizing L-tryptophan from the beginning completely by taking methanol as a carbon source, effectively gets rid of the dependence of a traditional process on grain resources, reduces the production cost, and provides a brand new technical path for industrial production of L-tryptophan.

The invention concerns synthetic miRNAs targeting the CISH gene and uses thereof. Pharmaceutical compositions comprising such miRNAs and uses thereof in the medical field, in particular in the treatment of cancer diseases and in immunotherapy, are also described.

The invention discloses a construction method and application of a pichia pastoris engineering strain for highly expressing mannase. The heat-resistant mannase derived from thermophilic aerobic bacteria is taken as a research object, and the characteristics of high enzymatic activity, high temperature resistance, wide temperature action range and the like of the heat-resistant mannase are fully exerted. A genetically engineered bacterium capable of stably and efficiently expressing mannase is finally screened out through methods of optimizing a promoter and a signal peptide, increasing the dose of a target gene, co-expressing protein expression related factors and the like, the highest enzyme activity can reach 5635.43 U / ml after 120-hour shake flask fermentation, and a good application prospect is shown.

Compositions and methods, systems, and kits for detecting and quantifying variations in numbers of molecules, particularly variations in gene dosage, e.g., due to gene duplication, or to variations from the normal euploid complement of chromosomes, e.g., trisomy of one or more chromosomes that are normally found in diploid pairs, without digital sequencing.

The invention belongs to the technical field of bioengineering, and relates to an engineering strain for efficiently expressing aspergillus niger alpha-glucosidase and a construction method of the engineering strain. The engineering strain comprises an alpha-glucosidase optimized gene expression cassette derived from aspergillus niger, a gene expression cassette for optimizing signal peptide and a double-promoter system, the nucleotide sequence of the alpha-glucosidase optimized gene is as shown in SEQ ID NO. 1 in a sequence table; the nucleotide sequence of the optimized signal peptide is shown as SEQ ID NO.5 in a sequence table; the double promoter system is composed of a methanol inducible AOX1 promoter and a constitutive GAP promoter. The optimal alpha-glucosidase expression strain is obtained by adopting a strategy of optimizing codons, gene doses, signal peptides and difunctional double promoters, and the fermentation level of the strain is remarkably improved compared with that of the strain before optimization; the enzyme shows excellent isomaltooligosacharide synthesis capability, and a reliable technical basis is provided for large-scale application in the fields of food processing, biological energy sources and the like.

The invention discloses a sweet potato excellent genotype dose effect detection technology and application, and belongs to the technical field of plant genetic engineering. Whole genome association analysis, haplotype analysis and a Hi-TOM deep sequencing technology are integrated, the relationship between the genotype and dose effect of functional genes in hexaploid sweet potatoes and the functional genes is excavated, excellent haplotypes of sweet potato root rot associated genes IbCHYR1 and soft rot associated genes IbERF114 and IbNF-YA3 are obtained, and the gene dosage of the excellent haplotypes is measured, so that the gene dosage of the sweet potato root rot associated genes IbCHYR1 and the soft rot associated genes IbERF114 and IbNF-YA3 is determined, and the content of the sweet potato root rot associated genes IbCHYR1 and the soft rot associated genes IbNF-YA3 is determined. Whether the gene has the function of resisting sweet potato root rot or soft rot or not can be determined, and an important theoretical basis and a practical tool are provided for genetic breeding of sweet potatoes.

The invention concerns synthetic miRNAs targeting the TOX gene and uses thereof. Pharmaceutical compositions comprising such miRNAs and uses thereof in the medical field, in particular in the treatment of cancer diseases and immunotherapy, are also described.

The invention relates to a gene dose effect target for improving the tolerance of yeast and application, and particularly provides a group of gene combinations YCL051W-YCL047C capable of enhancing the heat resistance of the yeast, and the gene combinations include YCL051W, YCL050C, YCL049C, YCL048W and YCL047C. A yeast engineering bacterium with heat resistance can be constructed by overexpressing a gene combination YCL051W-YCL047C in a yeast strain, the target combination is suitable for various different types of industrial strains, and the modified strain shows enhanced heat resistance and can accelerate microbial metabolism, improve the production efficiency and remarkably reduce the industrial fermentation cost; meanwhile, the production process efficiency and stability are improved, and the contamination risk is reduced.