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Echinacea adventitious bud regeneration culture method

A technology for regenerative culture and regeneration medium, applied in the field of plant biology, can solve problems such as inhibiting the regeneration efficiency of adventitious buds, and achieve the effects of improving the regeneration efficiency of adventitious buds, good economic and social benefits, and broad application prospects.

Inactive Publication Date: 2015-11-18
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the improved technology, adding a specific concentration of DA-6 to the original medium helps to improve the regeneration efficiency of adventitious buds. However, when cultivating some biomass types of explants, DA-6 will inhibit the regeneration of adventitious buds. regeneration efficiency

Method used

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  • Echinacea adventitious bud regeneration culture method
  • Echinacea adventitious bud regeneration culture method
  • Echinacea adventitious bud regeneration culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1. Explant preparation: Use scissors to cut several leaves, petioles and roots from the complete Echinacea purpurea seedlings cultured. After rinsing the collected leaves, petioles and roots with tap water for 30 minutes, put all the leaves, petioles and roots in a glass beaker with a capacity of 1000 mL on the ultra-clean workbench, and add 75% ethanol solution to the beaker until all the leaves are submerged. After 1 minute, pour off the ethanol solution, and keep the leaves, petioles and roots; then add 2% sodium hypochlorite solution to the beaker until all the leaves, petioles and roots are submerged. After 20 minutes, pour off the sodium hypochlorite solution, keep the blade, petiole and root; then add sterile distilled water to the beaker until all the leaves, petiole and root are submerged, rinse the blade, petiole and root for 2min, pour off the distilled water, and keep the blade, petiole and root. After rinsing the leaves, petioles and roots with sterile wate...

Embodiment 2

[0028] Experimental method is with embodiment 1, and experimental result is from table 1 and figure 1 It can be seen that leaf explants of different sizes performed differently on the regeneration medium containing DA-6. In all 5 DA-6 concentrations tested, small explants (4 mm × 4 mm = 16 mm 2 ), middle explant (7mm×7mm=49mm 2 ) and large explants (10mm×10mm=100mm 2 ) of adventitious bud induction first increased and then decreased, showing that the addition of appropriate concentration of DA-6 helps to promote the production of adventitious buds on leaf explants, and the concentration of DA-6 required for the best induction efficiency of large explants 0.08mg·L -1 , which is 0.01mg·L higher than the concentration required for the optimal induction rate of small explants -1 .

[0029]

Embodiment 3

[0030] Effect of embodiment 3DA-6 on the regeneration efficiency of petiole explants of different biomass

[0031] Experimental method is with embodiment 1, and experimental result is from table 2 and figure 2 It can be seen that petiole explants of different sizes performed differently on the regeneration medium containing DA-6. Among the five DA-6 concentrations tested, as the concentration of DA-6 increased, the explants of three sizes showed that the induction of adventitious buds first increased and then decreased, that is, adding an appropriate concentration of DA-6 helped To promote adventitious bud formation on petiole explants. The best concentration of large explants is 0.08mg·L -1 , higher than the optimal DA-6 concentration of 0.01mg·L for small explants and medium explants -1 . When the concentration of DA-6 is 0.08mg·L -1 , the adventitious bud induction rate of large explants was at the highest induction rate, but the adventitious bud induction of small ...

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Abstract

The invention belongs to the technical field of plant biology, and particularly discloses an echinacea adventitious bud regeneration culture method. DA-6 of different concentrations is added in a regeneration culture medium according to explants of different biomasses and sources. The echinacea adventitious bud regeneration efficiency can be remarkably improved, the problem that the regeneration efficiency of some explants with small biomasses is restrained when DA-6 is added only according to the gene dosage and explant sources originally is avoided, and the purpose of further improving the adventitious bud regeneration efficiency is achieved. The method has wide application prospects and good actual application value, and can generate good economic benefits and social benefits.

Description

technical field [0001] The invention relates to the field of plant biotechnology, in particular to a method for regeneration and cultivation of adventitious buds of Echinacea purpurea. Background technique [0002] Echinacea ( Echinacea purpurea L.) is a perennial herb of Compositae, which has a good immune regulation effect. Its extract is an immune enhancer and immune regulator that has received widespread attention in the world in recent years. Echinacea has become a world-renowned medicinal plant due to its precise properties. In order to obtain multiple varieties of Echinacea purpurea, people have tried to apply a variety of biotechnological means to Echinacea purpurea, such as transgenic, anther culture, and chromosome doubling. The achievement of these goals must go through a common step, that is, to induce the culture to produce adventitious buds. Although many studies have been carried out on the regeneration of adventitious buds of Echinacea purpurea in the prio...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 杨跃生陈晓鹭李栋梁吴鸿姜伟珍
Owner SOUTH CHINA AGRI UNIV
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