159 results about "Heat labile" patented technology

An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72, numbered relative to SEQ ID NO:1, of the A subunits mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E. coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.

Hardenable and hardened dental compositions, and articles including such hardenable and hardened compositions, are provided. The hardenable dental compositions include a thermally labile component including one or more thermally labile groups. Upon heating, the hardened compositions are useful, for example, for reducing the bond strength of orthodontic appliances adhered to tooth structures with the hardened compositions.

The invention aims at acid, alkali and heat labile features of sinomenine molecular structure, and provides an innovative structural reconstruction idea for new chemical synthesis of ring A, ring C, and ring D sinomenine derivatives. The chemical synthetic method includes amination and acylation of 1-position, C-C or C-O connection of 1 position, dicarbonylation and six-membered N heterocyclization of 3-position and 4-position, two molecule sinomenine adduction of connection of 4-hydroxyl and 1-amino, amination and acylation of 6-position, simultaneous amination and x-membered N heterocyclization of 6-position and 7-position, and opening of D ring and terminal amino group modification thereof. The method is novel and unique. The sinomenine derivatives have good antiinflammation activity and bioactivity evaluated by synovial membrane tumor cell (SW982), and can be used in drug and health products for resisting rheumatoid arthritis.

Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and / or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and / or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.

The invention relates to a novel IR laser desorption / vacuum UV single-photon ionization mass spectrum analyzing device for analysis and detection of a biological sample, which solves the problems of the prior art that uses matrix and is unlikely to analyze complex mixture with low polarity and low molecular weight. The device is structurally characterized in that a photoionization chamber is arranged on one side of a time-of-flight mass spectrometer, an ion injection hole is provided on one sidewall of the photoionization chamber, an ion injector and a sample target are arranged in the chamber, a laser is disposed outside the photoionization chamber on the sample target side, and vacuum UV light source is arranged on the other side. By combining IR laser desorption technology and vacuum UV ionization mass spectrum technology, the invention can achieve high-efficiency and rapid analysis without selecting different matrixes according to the sample and without complex pre-treatment and separation of the sample, thus preventing the matrix interference on mass spectral signals. The invention is specifically suitable for analysis of thermal-unstable and nonvolatile organic compounds and biological molecules.

The present invention provides parenteral adjuvants comprising detoxified mutants of bacterial ADP-ribosylating toxins, particularly those from pertussis (PT), cholera (CT), and heat-labile E. coli (LT).

Disclosed in certain embodiments is a solid oral dosage form comprising a heat-labile gelling agent; a thermal stabilizer; and a drug susceptible to abuse.

Processes and methods exist for decreasing emissions of mercury upon combustion of fossil fuels such as coal. Halide salts can be effective when used at locations where they are thermally decomposed to form reactive halogen species, or in combination with an adsorbent material such as activated carbon. Halide salts, such as calcium bromide and sodium bromide, are not typically used at locations downstream of the economizer, where the temperature is typically below around 500° C., because these salts are non-thermolabile and do not decompose to produce reactive halogen species. However, in flue gas streams that certain flue gas constituents, such as sulfur trioxide or sulfuric acid, reactive halogen species can be produced via chemical reaction. These species react with elemental mercury through various means to form an oxidized form of mercury that is more easily captured in downstream pollution control devices such as particulate control devices or SO2 scrubbers.

A method of packaging thermally labile goods. The method includes the steps of (a) obtaining a legend correlating thermally labile goods with a PCM panel color, (b) locating the thermally labile goods to be packaged on the legend and identifying the correlated PCM panel color, (c) obtaining thermally conditioned panels of the correlated PCM panel color from amongst a plurality of differently colored thermally conditioned panels color coded in relation to the phase change temperature of the phase change material contained within the panels, (d) lining the retention chamber of a thermally insulated container with the obtained thermally conditioned PCM panels, (e) placing the thermally labile goods to be packaged into the lined retention chamber, and (f) closing the container.

The ability to incorporate iodoform, tetracycline and a combination of iodoform / tetracycline into root canal gutta percha points is described. The iodoform, tetracycline and iodoform / tetracycline combination are bound within the gutta percha points. They act as a reservoir of antimicrobial that is capable of diffusing onto the surface of the gutta percha thereby inhibiting the colonization of bacteria on the gutta percha points and within the root canal system. Tetracycline is capable of coalescing within the dentinal tubules to inhibit long term microbial growth. These medicated gutta percha points are site specific, surface acting antimicrobial gutta percha points.A method of the usage and delivery of amorphous form iodoform gutta percha, iodoform / tetracycline gutta percha, or tetracycline gutta percha within a heated compule with a pressure plunger by delivering the thermo-softened heat labile amorphous form via a pressure extrusion system through a cannula into the prepared root canal system.

The invention provides a low temperature synthesis method for a monodisperse polymer microsphere. The method includes the steps of 1) dissolving a free radical polymerization monomer, a stable dispersant, a reversible addition-fragmentation chain transfer agent (RAFT) and a photoinitiator in a reaction medium to obtain a mixture; and 2) feeding nitrogen to deoxidize and subjecting the mixture to a light reaction at a temperature between 0 DEG C to 90 DEG C in a stirring state to obtain the monodisperse polymer microsphere. According to the method, on a basis of light dispersion polymerization, a controllable / 'living' radical polymerization mechanism is introduced, and a control reagent of a free radical polymerization is added, so that a photopolymerization which is overquick previously is slowed down, a highly monodisperse polymer microsphere is obtained, and the problem that denaturation and effectiveness losing of heat labile components such as polypeptide and protein caused by a thermal-initiation polymerization under the long time heating in prior art are prone to occur is solved.

The invention discloses an automatic mixing device for a mixed feed additive. The automatic mixing device comprises four parts, namely a premixing system, a mixing system, a temperature control system and an automatic control system, wherein the premixing system comprises a premixing bin body, a main and auxiliary material feeding hole, a mixing blade, an adjustable motor and a fixed sealing flange; the mixing system comprises a mixing bin body, a mixing blade, an adjustable motor and a vibrator; the temperature control system comprises an internally inlet / outlet, a seal sheet and an interlayer; and the automatic control system comprises a relay switch gear, a connecting circuit and an automatic control board. The automatic mixing device has the characteristics of high automation, convenience in use, high main / auxiliary material mixing strength and obviously improved mixing degree, is high in device sealing degree, is capable of lowering the pollution rate and the material loss rate, reducing damage to human bodies, decreasing heat produced by machine operation and improving the activity and heat-labile component quality of the mixed feed additive, and is beneficial to quality stability of products in different batches.

The invention discloses a thermal shock gasifying electrospray ionization source and a mass spectrometry (MS) system, belonging to the technical field of ambient MS ionization. The MS ionization source disclosed by the invention comprises an electrospray generator, a heating device and a sampling device, wherein the heating device comprises a gasifying chamber and a heater providing heat for the gasifying chamber; a sample nozzle of a sample tube of the sampling device is configured in a way of facing the bottom of the gasifying chamber; the outlet end of a spraying capillary tube of the electrospray generator is opposite to the inlet of a mass spectrometer and is spaced from the inlet by a certain distance; the gasifying chamber and the sample nozzle are placed under the space between the outlet end of the spraying capillary tube and the inlet of the mass spectrometer. The ionization source provided by the invention gasifies a sample in a thermal shock manner and improves the heating efficiency of the sample through shortening heating time; since the sample is heated for a short time, heat energy is not converted into the molecular internal energy of the sample and only can be provided for the sample to overcome the intermolecular force, and the molecules of the sample cannot be decomposed due to heating; thus, the ionization source provided by the invention can be used for detecting heat-unstable and nonvolatile compounds and biological samples and solves the difficulty existing in the traditional thermal desorption technology.

Processes and methods exist for decreasing emissions of mercury upon combustion of fossil fuels such as coal. Halide salts can be effective when used at locations where they are thermally decomposed to form reactive halogen species, or in combination with an adsorbent material such as activated carbon. Halide salts, such as calcium bromide and sodium bromide, are not typically used at locations downstream of the economizer, where the temperature is typically below around 500° C., because these salts are non-thermolabile and do not decompose to produce reactive halogen species. However, in flue gas streams that certain flue gas constituents, such as sulfur trioxide or sulfuric acid, reactive halogen species can be produced via chemical reaction. These species react with elemental mercury through various means to form an oxidized form of mercury that is more easily captured in downstream pollution control devices such as particulate control devices or SO2 scrubbers.

The present invention is low temperature process of preparing anatse type crystalline titania film, and belongs to the field of wide-gap semiconductor photocatalyzing technology. Titania film is deposited on the substrate through reactive magnetically controlled sputtering, and the deposition process has the base with the substrate cooled by a water cooling system, argon flow rate of 30 sccm, the flow rate between argon and nitrogen of 5-10, total gas pressure of 1-4 Pa, power density of 1.4-2.8 W / sq cm, negative voltage applied on the base of 20V-100, and base temperature always lower than 80 deg.c. During the film depositing process, great amount of positive argon ion is driven by the base bias voltage to bombard the deposited titania film so as to form anatse type crystalline titania film on the heat labile substrate.

The invention discloses a 3D porous electrode preparation method and a use of a 3D porous electrode in electrochemical hydrogen evolution and belongs to the field of electrochemistry. The method comprises repeatedly impregnating a commercial carbon cloth with a transition metal salt, putting the commercial carbon cloth with the transition metal salt and a heat-labile nitrogen-containing monomer into a porcelain boat, and carrying out stepwise calcining treatment to obtain the 3D porous electrode comprising carbon tubes growing on the activated carbon cloth. The method has simple processes, less equipment investment and small batch difference and is suitable for large scale production. The 3D porous electrode has well-developed tunnel distribution, a high specific surface area and excellent conductivity. The 3D porous electrode can be used in the water electrolysis oxygen-evolution hydrogen-evolution reaction, can test hydrogen evolution reaction activity at a low temperature and has excellent catalytic performances.

The invention relates to a method and a system for continuously preparing 5-hydroxymethylfurfural (5-HMF) and a derivative 5-alkoxymethylfurfural (5-AMF) thereof with hexose or hexose-containing biomass as a material. The method takes the characteristic of thermal instability in a target product into full consideration and utilizes the characteristic of distinct polarity difference between the product and the material, a reaction unit and an extraction unit are separated in the process of preparing the 5-HMF and the 5-AMF, the biomass material reacts along with a polar reaction phase under high temperature and existence of a catalyst, the produced product flows out of reaction environment in time, and after being cooled, the product is extracted and retained by a non-polar extraction phase under normal temperature and pressure. The insufficiently reacting material or a polar intermediate product is retained in the polar reaction phase, and enters the reaction unit again to form circulation until the reaction is sufficient. In order to increase the distribution ratio and extraction efficiency of the product, a certain amount of inorganic salt can be added into a polar solvent. The method and the device effectively increase the selectivity and yield of the target product, and remarkably reduce the energy consumption of the whole preparation process. Material resources applicable to the method and the system are wide, including soluble monosaccharide, insoluble lignocellulose and the like. Pickiness about catalysts is avoided, and homogenous and heterogeneous catalysts which are commonly used in such reaction are all applicable to the method and the system.

Disclosed is a top coating composition formed on a resist film, for protecting the resist film, the top coating composition being a top coating composition for photoresist, characterized by containing a fluorine-containing polymer having a repeating unit represented by the following general formula (1). This composition is capable of controlling developing solution solubility and has a high water repellency.[In the formula, R1 represents a hydrogen atom, fluorine atom, methyl group or trifluoromethyl group, R2 represents a heat-labile protecting group, R3 represents a fluorine atom or fluorine-containing alkyl group, and W is a bivalent linking group.]

The inventive subject matter relates to an immunogenic composition composed of a chimeric molecule including a conformationally stable adhesin from Escherichia coli fused to a bacterial toxin A subunit, such as cholera toxin A subunit or heat-labile Escherichia coli toxin A subunit. The invention also relates to the adhesin-toxin chimera noncovalently associated with a toxin B subunit of the same or different species as the A subunit. The invention also relates to a method of utilizing an adhesin / toxin fusion composition to elicit an immune response.

Compound methilanin thiamin is used to assist treat that acute and chronic hepatitis, fatty liver, early cirrhosis of liver, toxic hepatitis brought by arsenic and barbiturate and other diseases in clinic. But, there is only liquid drugs injection supplied in market at present. Compound methilanin thiamin is unstable to heat; thiamin is easy to decompose on condition of solution, opacification or precipitate; after sterilization at high temperature, content of thiamin in liquid drugs injection will decrease for decomposition; meantime the storage and transport for liquid drugs injection is not convenient. The invention overcomes the shortage of methilanin thiamin liquid drugs injection, increases stability of drug, is propitious to transport and storage; and avoids freezing easily in winter and affecting quality of drug, prolongs drug storage life, improves quality of drug, in order to treat patients more safely and effectively.

An immunogenic detoxified protein is provided which comprises the amino acid sequence of subunit A of an E. coli heat labile toxin (LT-A) or a fragment thereof in which at least amino acid Ala-72 of the A subunit is mutated, preferably by substitution with Arg. The toxoid is useful as vaccine against an enterotoxigenic strain of E. coli and is produced by recombinant DNA means by site-directed mutagenesis. It is also an effective adjuvant.

A GC-IMS system is disclosed in embodiments of the present invention. The system comprises a sample transfer device. The sample transfer device connects the gas chromatograph to the reaction region and, the sample from the gas chromatograph is transferred to the reaction region by the sample transfer device directly, instead of not through the ionization region. With the GC-IMS system, generation of sample molecular ion fragments can be avoided so that the spectrum is brevity and is easily identified; moreover, the application field of the GC-IMS system is extended to a range of analysis of organic macromolecule samples which have a high polarity, are difficult to volatilize, and are thermally instable. On the other hand, the GC-IMS system overcomes the defect of ion destruction due to neutralization reaction among positive and negative ions so as to evade the detection.

Provided herein are methods and compositions for nucleic acid replication. These methods involve the use of 3′-substituted nucleoside 5′-triphosphates or 3′-substituted terminated primers in nucleic acid replication reactions. In certain aspects, the methods are accomplished by use of 3′-substituted NTPs and / or 3′-substituted terminated primers which provide utility in nucleic acid replication. In preferred embodiments, the NTPs and / or primers are substituted at the 3′-position with particular heat labile chemical groups such as ethers, esters or carbonate esters.

The present disclosure is directed to compositions and methods for making a pharmaceutical composition by thermokinetic compounding, wherein the compositions include one or more thermolabile components, for example one or more active pharmaceutical ingredients (API) with one or more pharmaceutically acceptable excipients. The methods comprise thermokinetic processing of the thermolabile components into a composite by blending certain thermolabile components in a thermokinetic mixer using multiple speeds during a single, rotationally continuous operation. The composite can be further processed into pharmaceutical compositions by conventional methods known in the art, such as hot melt extrusion, melt granulation, compression molding, tablet compression, capsule filling, film-coating, or injection molding.

Provided is a hydroxyl-protected alcohol comprising a thermolabile hydroxyl-protecting group comprising a 2-pyridyl substituent and a precursor of the thermolabile hydroxyl-protected alcohol. An exemplary thermolabile hydroxyl-protected alcohol is represented by the formula Pg-O—R, wherein Pg is a protecting group of the formula: (Formula) wherein: A is a 2-pyridyl; Z is CH2 or NR1; R1, R2, R2′, R3 and R3′ are the same or different and each can be, e.g., H, alkyl, or alkyl comprising an aryl substituent; W is CO, CS, or SO; and R is the organic residue of the hydroxyl-protected alcohol. Also provided is a method of producing an alcohol, which method comprises heating the hydroxyl-protected alcohol, which optionally may be obtained from a precursor, at a temperature effective to cleave the hydroxyl-protecting group. The method can be used to produce oligonucleotides.

The invention relates to a polysaccharide conjugate vaccine, which comprises a chemical conjugate consisting of a bacterial polysaccharide and a vector protein, wherein the vector protein is a bacterial slime invasion-associated protein, such as a bacillus coli heat-labile toxin B subunit, a campylobacter jejuni flagellum secretion protein A1, a campylobacter jejuni flagellum secretion protein A2, a pneumococcus surface protein and a pneumococcus surface adhesin. The invention further relates to a preparation method for the polysaccharide conjugate vaccine. According to the invention, the mucosa delivery of the polysaccharide conjugate vaccine can be realized on the premise of not adding any other extrinsic protein; and due to the adoption of an immune route, the vaccine is more efficient, convenient and safe to use.

The invention relates to an o-phthalaldehyde disinfectant and a preparation method thereof. The o-phthalaldehyde disinfectant comprises the following components in percentage by weight: 0.2 to 1 percent of o-phthalaldehyde, 0.1 to 0.5 percent of salicylic acid, 0.5 to 1.5 percent of dodecyldimethylbenzylammonium chloride, 1 to 10 percent of ethanol, 0.1 to 1 percent of disodium hydrogen phosphate, 1 to 10 percent of ethanol, 0.1 to 1 percent of sodium dihydrogen phosphate, 0.1 to 0.5 percent of inhibitor, 0.01 to 0.1 percent of ethylene diamine tetraacetic acid and the balance of water. The method for preparing the disinfectant comprises the steps of: adding the o-phthalaldehyde into the ethanol according to the content and component, stirring and mixing the materials; adding water into the materials and stirring the mixture; and adding the dodecyldimethylbenzylammonium chloride, the disodium hydrogen phosphate, the sodium dihydrogen phosphate, the inhibitor and the ethylene diamine tetraacetic acid into the mixture in sequence and stirring the mixed solution to allow the added materials to dissolve to obtain the o-phthalaldehyde disinfectant. The disinfectant has the advantages of no pungent smell, safety, low toxicity, stable property and convenient use, can be used for high-level disinfection of the heat-labile medical equipment and is particularly applicable to an endoscope automatic washing and disinfecting machine to perform high-level disinfection on an endoscope.