4 results about "Heat labile" patented technology

The present invention relates to enzymes having DNA polymerase and 3′-5′ exonuclease activities. In particular, the present invention relates to a heat labile enzyme possessing a DNA polymerase II activity and a 3′-5′ exonuclease activity of marine origin. Furthermore, the present invention relates to a DNA polymerase primarily exerting a 3′-5′ activity, i.e. where the polymerase activity is absent. The present invention furthermore relates to the use of the exonuclease activity to degrade the 3′-5′ strand of double stranded DNA to perform single stranded overhang, e.g. in recombinant cloning processes, or in processes for removal of contaminating nucleic acid molecules.

A method and material for 3D printing objects containing heat-sensitive molecules are disclosed. The composition consists of a water-saturated, polar thermoplastic polymer, such as highly hydrolyzed polyvinyl alcohol (PVA), into which functional molecules—proteins, peptides, pharmaceuticals, fragrances, or living cells—are dispersed. The polymer is first hydrated to form a gel, extruded and cut into pellets, then partially dried through a multi-stage dehydration process that leaves a moisture-rich core and a dry outer shell. During extrusion at typical FDM nozzle temperatures (≈200° C.), entrapped water within the material vaporizes at ≈100° C., providing in situ evaporative cooling and maintaining the temperature of the functional molecules below their degradation point. The process enables additive manufacturing of structurally sound objects that preserve the activity of heat-sensitive additives, opening new applications for 3D-printed consumables, pharmaceuticals, and bio-functional components.

The present invention provides a heat-stable enterovirus group A71 strain, screening methods, and applications thereof. The heat-stable enterovirus group A71 strain, deposited with CCTCC No. V202233, exhibits stable cell growth after heat treatment at 52°C and exhibits high yield and high immunogenicity. Compared to wild-type or heat-unstable strains, the heat-stable strain exhibits five nucleotide mutations in its coding region, including three missense mutations and two synonymous mutations. This strain can be used in the screening and modification of inactivated vaccine strains, as well as in vaccine production.

The application discloses a terminal modified deoxynucleotide, and belongs to the technical field of DNA polymerization. The structural formula of the deoxynucleotide is as follows: wherein R is at least one of the following groups:,,, and. The terminal modified deoxynucleotide improves the stability of the deoxynucleotide in storage and in a PCR process, and the deoxynucleotide has the characteristics of heat instability, can be hydrolyzed to generate dNTP after being heated, can more long-term supplement raw materials required in the PCR process, and thus realizes amplification of a long DNA fragment, and has a wide application prospect.