6 results about "Bacterial vaccine" patented technology

The invention relates to a vaccine freeze-drying protective agent as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The vaccine freeze-drying protective agent is prepared from the following raw materials: trehalose, mannitol, arginine, pullulan, 2-hydroxypropyl-beta-cyclodextrin, phospholipid, sodium sulfite and a protective additive, the protective additive is at least one of hydroxyl ectoine, betaine, dodecyl dimethyl betaine and polycarboxylic acid betaine. The preparation method comprises the following steps: shearing and mixing the 2-hydroxypropyl-beta-cyclodextrin and the phospholipid at a high speed, sequentially adding the other components, adjusting the pH and osmotic pressure, performing sterile filtration, and performing freeze drying. The protective agent can effectively inhibit phase separation, sugar recrystallization and interfacial adsorption in the freeze-drying process, significantly improves the activity retention rate and physical stability of vaccines in the freeze-drying and storage periods, and is suitable for preparing freeze-drying preparations of various vaccines such as viral vaccines, bacterial vaccines and recombinant protein vaccines.

A vaccine comprising a live avirulent strain of bacteria or an immunogenic composition that comprises a live avirulent strain of bacteria, wherein the bacteria is cured of plasmids and / or transposons that contain AMR genes, and a non-conjugative synthetic curing plasmid that cures plasmids and / or transposons that contain AMR genes from such bacteria. A method for producing the live avirulent strain of bacteria or an immunogenic composition that comprises a live avirulent strain of bacteria; a method for treating, preventing, or reducing the severity, duration, or incidence of clinical signs, of a virulent bacterial infection; and a method for preventing, or reducing the risk or incidence of transference of AMR genes are also disclosed.

The present application relates to a vaccine freeze-drying protective agent, its preparation method and application, and belongs to the technical field of biological medicine. The vaccine freeze-drying protective agent comprises the following raw materials: trehalose, mannitol, arginine, pullulan, 2-hydroxypropyl-beta-cyclodextrin, phospholipid, sodium sulfite and a protective additive; the protective additive is at least one of hydroxyectoin, betaine, dodecyl dimethyl betaine and polycarboxylic betaine. The preparation method comprises the following steps: mixing 2-hydroxypropyl-beta-cyclodextrin and phospholipid by high-speed shearing, sequentially adding the remaining components, adjusting pH and osmotic pressure, and then performing freeze-drying after sterile filtration. The protective agent can effectively inhibit phase separation, sugar recrystallization and interface adsorption during the freeze-drying process, significantly improve the activity retention rate and physical stability of the vaccine during freeze-drying and storage, and is suitable for the preparation of freeze-drying preparations of various vaccines such as viral vaccines, bacterial vaccines and recombinant protein vaccines.

The present document discloses a method for producing a bacterial vaccine composition against bacterial diseases in animals, such as tetrapods or fish, wherein said bacterial vaccine composition comprises inactivated bacteria of the bacterium causing said bacterial diseases. The method involves exposing the bacteria to different growth conditions and then preparing a bacterial vaccine composition where the bacteria are exposed to different growth conditions. With vaccines prepared by the present method, bacterial diseases that previously had not been able to be cured could be treated or the effect of the treatment was more long-lasting.

The invention belongs to the technical field of biological medicine, and particularly relates to polyamino acid, polyamino acid nanoparticles, a polyamino acid engineered bacterial vaccine and a preparation method. The polyamino acid provided by the invention has a structure as shown in formula (I). The polyamino acid provided by the invention can be prepared into nano particles and further prepared into bacterial vaccines. Nanoparticles prepared from the polyamino acid provided by the invention can have good monodispersity, have amino-terminated groups and rich positive charges on the surfaces, can be grafted on the surfaces of bacteria in an amide condensation manner, and then are used as vaccines by efficiently loading different antigens through electrostatic adsorption; the polyamino acid engineered bacterial vaccine prepared from the nanoparticles has no toxic or side effect on human bodies, has the function of promoting activation of antigen presenting cells, can promote maturation of dendritic cells in vivo and further initiate strong adaptive immune response, and has a good immune activation effect. Formula (I).

The invention discloses a vaccine composition which comprises a bacterial antigen and at least one drug carrier, the drug carrier is a saponin-containing polymer microsphere which is formed by freezing and thawing a polymer microsphere with a cavity structure so as to form an opening or a pore on the surface of the polymer microsphere, the preparation method of the vaccine composition comprises the following steps: mixing the drug carrier with a solution containing bacterial antigens, preferably enabling the bacterial antigens to be loaded on the drug carrier in modes of infiltration and / or adsorption and the like, and then centrifuging to remove supernate so as to obtain the polymer microspheres loaded with the antigens.