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Aquaculture creature disease controlling compound vaccine and its preparation method

An aquaculture, compound bacteria technology, applied in the direction of animal repellents, botanical equipment and methods, antibacterial drugs, etc., to achieve the effect of extensive control

Inactive Publication Date: 2006-04-26
DALIAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although injection and bath vaccination have a certain degree of immune protection, for aquaculture, it is almost impossible to catch diseased fish and shrimp for injection and bath vaccination

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Preparation of a mixed vaccine consisting of 50% Vibrio alginolyticus and 50% Aeromonas hydrophila.

[0029] 1. Prepare single-component bacterins of Vibrio alginolyticus and Aeromonas hydrophila respectively:

[0030] (1) Vibrio alginolyticus and Aeromonas hydrophila strains were respectively inoculated on the culture medium, and cultured at 25-37° C. for 24-48 hours.

[0031] (2) The culture is washed with physiological saline and inactivated with 1-5% formaldehyde solution.

[0032] (3) After centrifuging the inactivated culture at 3000-5000 rpm, dissolve it with physiological saline, then centrifuge again, and repeat it several times to remove the remaining formaldehyde solution.

[0033] (4) Dissolving the precipitate obtained by the last centrifugation with physiological saline to obtain a single-component bacterin.

[0034] 2. Mix the prepared single-component bacterins according to the proportion concentration of 50% of the formula, and the total amount of bac...

Embodiment 2

[0037] Preparation of a mixed vaccine consisting of 25% of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio anguillarum and Aeromonas hydrophila.

[0038] 1. Prepare single-component bacterins of Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio anguillarum and Aeromonas hydrophila respectively:

[0039] (1) Inoculate the culture medium with Vibrio alginolyticus, Vibrio parahaemolyticus, Vibrio anguillarum and Aeromonas hydrophila respectively, and culture at 25-37°C for 24-48 hours.

[0040] (2) The culture is washed with physiological saline and inactivated with 1-5% formaldehyde solution.

[0041] (3) After centrifuging the inactivated culture at 3000-5000 rpm, dissolve it with physiological saline, then centrifuge again, and repeat it several times to remove the remaining formaldehyde solution.

[0042] (4) Dissolving the precipitate obtained by the last centrifugation with physiological saline to obtain a single-component bacterin.

[0043] 2. Mix the prepared ...

Embodiment 3

[0046] Vibrio alginolyticus, Aeromonas hydrophila, Aeromonas sobria, Pseudomonas aeruginosa, and Pseudomonas shigella-like accounted for 20% of the mixed vaccine.

[0047] 1. Prepare single-component bacterins of Vibrio alginolyticus and Aeromonas hydrophila, Aeromonas sobria, Pseudomonas aeruginosa, and Pseudomonas shigellai respectively:

[0048] (1) Inoculate the culture medium with Vibrio alginolyticus, Aeromonas hydrophila, Aeromonas sobria, Pseudomonas aeruginosa and P. ~48 hours.

[0049] (2) The culture is washed with physiological saline and inactivated with 1-5% formaldehyde solution.

[0050] (3) After centrifuging the inactivated culture at 3000-5000 rpm, dissolve it with physiological saline, then centrifuge again, and repeat it several times to remove the remaining formaldehyde solution.

[0051] (4) Dissolving the precipitate obtained by the last centrifugation with physiological saline to obtain a single-component bacterin.

[0052] 2. Mix the prepared singl...

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PUM

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Abstract

Disclosed is an aquaculture creature disease controlling compound vaccine which mainly comprises vibrio alginolyticus, bibrio parahemolyticus, vibrio alginolyticus, Aeromonas hydrophila, Aeromonas sobria, Pseudomonas aeruginosa, and Vibrio alginolyticus. The invention also discloses the preparing process for the composite bacterial vaccine.

Description

technical field [0001] The invention relates to a compound bacterial vaccine for preventing and treating aquaculture diseases. Background technique [0002] Bacterial diseases are common diseases in seawater culture such as prawns, fish, sea cucumbers, etc. For example, the pathogens of shrimp red leg disease (red leg disease) include: Vibrio parahaemolyticus, Vibrio eel, and Vibrio alginolyticus; the pathogen of eye rot There are: non-01 Vibrio cholerae; gill rot pathogens: Vibrio, Pseudomonas, Aeromonas; brown spot pathogens: Vibrio, Pseudomonas, Spirillum, Flavobacterium The pathogens of Aeromonas disease are: Aeromonas; the pathogens of vibrio diseases of prawn larvae are: Vibrio eel, Vibrio parahaemolyticus, Vibrio alginolyticus; and these bacteria can also cause breeding diseases such as fish and sea cucumbers Such as: canker disease, gill rot disease, etc. of fish cultured in sea water; skin rot, ulcer, etc. of sea cucumbers. These bacterial diseases often lead to m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A61K35/74A61P31/04
Inventor 刘为国
Owner DALIAN UNIV
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