30 results about "Aeromonas sobria" patented technology

The invention relates to a novel feed additive. The feed additive is an antibacterial agent containing a condensation compound derived from fatty acid and esterified binary amino acids and has the advantages that riemerella anatipestifer, RA diseases of ducklings caused by riemerella anatipestifer, RA can be prevented and treated, peritonitis diseases caused by escherichia coli and staphylococcusaureus can be treated, and septicemia of fish caused by aeromonas sobria can be prevented and treated; bacteria can be effectively inhibited or killed, the risk that poultry, livestock and aquatic animals are infected with the bacteria can be greatly reduced, and the survival rate of the animals infected with pathogenic bacteria can be increased; and ethyl lauroylarginate can be safely degraded ina human body and an animal body and is small in toxic and side effects, so that the generation of environmental drug-resistant bacteria caused by the fact that residual antibacterial agents are discharged into the environment can be avoided, and the negative influence on the environment is less while the effective antibacterial effect is achieved.

Immobilized cell beads incorporating formulated microbial consortium comprising a synergistic mixture of the following bacterial strains namely, Enterobacter sakazaki, Pseudomonas aeruginosa and Aeromonas sobria selected from the following isolated bacterial strains namely, Yersinia enterocolitica, Aeromonas sobria, Klebsiella pneumoniae, Serratia liquefaciens, Enterobacter sakazaki, Citrobacter amalonaticus, Pseudomonas fluorescens, Pseudomonas aeruginosa, Enterobacter cloaca, Acinetobacter calcoaceticus are prepared, the formulated microbial consortium is immobilized in an appropriate immobilizing agent resulting in the formation of beads and the beads are used for instant BOD estimation using an electronic device and the formulated cell beads are reusable and are capable of assimilating most of the organic matter present in varied industrial effluents.

The invention discloses a preparation method of a loach aeromonas sobria vaccine, which is characterized by comprising the following step: preparing a water aqua type loach aeromonas sobria vaccine using radix astragali as an adjuvant by using loach pathogenic aeromonas sobria LD081008A-1 as immunogen and a radix astragali lixivium as the adjuvant, wherein the concentration of the pathogenic aeromonas sobria as the immunogen in the vaccine is 2.7*108 cells / mL, and the concentration of the radix astragali lixivium is 1 g / mL. The invention further discloses a use method of the loach aeromonas sobria vaccine. By using the loach aeromonas sobria vaccine prepared by the method, the capability of the resistance of loaches to aeromonas sobria infection can be obviously improved; the immune protecting capability of the loaches to the pathogenic aeromonas sobria is enhanced; the survival rate of cultivating loaches is improved; the yield of cultivating the loaches is increased; the export obstacle caused by drug residue is avoided; the economic benefit in the cultivation production of the loaches is improved; and the problem that diseases caused by the aeromonas sobria easily appearing in the current cultivation production process of the loaches is solved foundationally.

The invention relates to Bacillus sp. S-2-6 CGMCC No.6477, which is obtained from the flowing steps: sampling a misgurnus anguillicaudatus sample in a misgurnus anguillicaudatus breeding farm, taking intestinal tract of the misgurnus anguillicaudatus sample, carrying out freezing grinding, filtering with gauze, taking the filtrate, carrying out gradient dilution, coating on a flat plate (TSB culture medium), culturing for 24 h at a temperature of 28 DEG C, selecting a typical single colony according to different characteristics such as colony morphology, a size, edge uniformity, transparency and the like, carrying out streaking, purifying, and storing in 15% glycerol at a temperature of -20 DEG C so as to separate the S-2-6 bacterial. The S-2-6 bacterial has effects of inhibitions of Aeromonas veronii, Aeromonas sobria and Vibrioharveyi.

The invention provides a double oral sustained-release microsphere vaccine of Aeromonas hydrophila and Aeromonas victorii and a preparation method thereof, belonging to the field of biopharmaceuticals. Sodium alginate was used as the wall material, and the inactivated whole bacteria of Aeromonas hydrophila and Aeromonas victoria were used as the core material. released microsphere vaccines. Using the Aeromonas hydrophila and Aeromonas victoria-calcium alginate microcapsule oral vaccine to immunize gibel crucian carp can significantly improve serum enzyme activity and leukocyte phagocytosis activity, and its relative protection rate against gibel carp is 46.7 %. The microcapsules are stable under simulated gastrointestinal conditions, showing good burst and sustained release properties; the bacterial antigenicity in the microcapsules remains good, and the vaccine safety and storage stability are good.

The invention discloses an LAMP detection primer combination of aeromonas sobria and a kit. The LAMP detection primer combination of aeromonas sobria comprises a pair of outer primers AS-F3 and AS-B3, a pair of inner primers AS-FIP and AS-BIP and a pair of loop primers AS-LF and AS-LB. the kit comprises the detection primer combination, BstDNA polymerase, an LAMP reaction solution, confining liquid mineral oil, a positive control and a negative control. The invention also discloses a method for detecting aeromonas sobria by the utilization of the above LAMP detection kit of aeromonas sobria. The kit provided by the invention is simple and rapid to operate, is suitable for field test, has high specificity, high detection sensitivity and high and reliable accuracy, and has a wide application prospect. The detection sensitivity of the kit can reach 320fg / mL.

The invention relates to a multiplex-PCR primer and an application thereof in the cultivation process of turbots. The multiplex-PCR primer consists of an aeromonas hydrophila specific primer H, an aeromonas sobria specific primer S and an aeromonas schubetii specific primer Ss. A method for rapidly identifying pathogenic bacteria in the cultivation process of turbots comprises the following steps: extracting total DNA of bacteria of a focus of turbots; by taking the extracted DNA as a template, performing PCR amplification by using the multiplex-PCR primer; and judging according to a sepharose gel imaging result of a PCR amplification primer. Through one time of PCR reaction, three common pathogenic bacteria in the cultivation process of turbots can be detected simultaneously, and very good specificity and sensitivity can be achieved. In the cultivation process of turbots, once the risk that the turbots are infected by the three aeromonas exists, the etiology can be rapidly identified, and cultivation households can be prevented from heavy loss.