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Freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine and industrialized preparation method thereof

A dual inactivated vaccine, the technology of Aeromonas hydrophila is applied in the direction of antibacterial drugs, bacterial antigen components, etc., to achieve the effects of low cost, prevention of hemorrhagic sepsis, and good immune effect

Inactive Publication Date: 2012-03-07
PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcomings of a single inactivated vaccine against only one pathogen, and provide a dual vaccine to achieve the effect of resisting two pathogens at the same time, and the vaccine can be fed by soaking, injection or mixing feed Easy to use

Method used

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  • Freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine and industrialized preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Example 1: Industrial preparation of Aeromonas freshwater fish double inactivated vaccine

[0017] 1. Primary seed preparation

[0018] Take out the strains (Aeromonas hydrophila, Aeromonas temperatus) stored in the refrigerator, inoculate nutrient agar (see the appendix for the medium formula), incubate at 28°C for 24 hours, use an inoculation stick to pick the moss and inoculate the nutrient agar, Cultivate at 28°C for 24 hours, inoculate 300ml / bottle of nutrient broth (see the appendix for the medium formula), 10 bottles, place on a shaker at 200rmp / min, cultivate at 28°C for 18 hours, and obtain 3L first-grade seeds.

[0019] The strains selected in this example are: Aeromonas hydrophila strain YYK090901, isolated from the kidney of bighead carp; Aeromonas sobria strain LYK090702, isolated from the kidney of silver carp; currently the strains are all preserved in the Pearl River of China Academy of Fishery Sciences The Fish Ward of the Fisheries Research Institute....

Embodiment 2

[0031] Embodiment two. Immune effect of double inactivated vaccine against Aeromonas in freshwater fish

[0032] Healthy crucian carp (30-50g / tail) were randomly divided into three groups, with 100 fish in each group. The first group is the injection immunization group, and each tail is injected intraperitoneally with 0.1ml of the double inactivated vaccine of Aeromonas freshwater fish prepared in Example 1; Soak for 3-5 minutes, and then inflate and soak for 10-15 minutes in the 10-fold diluted double vaccine solution; the third group is the control group. The water temperature is 22-26°C. After 28 days of immunization, 2 times the LD50 dose of Aeromonas hydrophila strain YYK090901 and Aeromonas temperatus strain LYK090702 were used respectively (Aeromonas hydrophila strain YYK090901 was isolated from bighead carp kidney, Aeromonas temperatus strain LYK090702 The bacteria were isolated from the kidney of silver carp, and the strains are currently stored in the fish ward of...

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Abstract

The invention relates to a bivalent inactivated vaccine, and specifically, relates to a freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine and an industrialized preparation method thereof. The freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine comprises inactivated antigen compositions of aeromonas hydrophila culture inactivated liquid and aeromonas sobria culture inactivated liquid. The invention also provides the industrialized preparation method of the freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine. The industrialized preparation method is characterized by comprising the following steps of mixing uniformly the aeromonas hydrophila culture inactivated liquid and the aeromonas sobria culture inactivated liquid according to a ratio of 1: 1, and carrying out aseptic loading to obtain freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine finished products. The freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine overcomes the defect that a monovalent vaccine has an effect only on a pathogen. The freshwater fish aeromonas hydrophila-aeromonas sobria bivalent inactivated vaccine has the effect of resisting two pathogens simultaneously by being applied once, and can be conveniently applied through immersion, injection or feeding after mixing with forage.

Description

technical field [0001] The invention relates to a dual inactivated vaccine, in particular to a dual inactivated vaccine of Aeromonas hydrophila and Aeromonas temperii for freshwater fish and a preparation method thereof. Background technique [0002] Freshwater fish bacterial hemorrhagic disease, also known as bacterial hemorrhagic septicemia, fulminant hemorrhagic disease, hemorrhagic ascites, etc., is one of the most serious infectious diseases that harm freshwater farmed fish. The harmful fish mainly include silver carp, bighead carp, Crucian carp, carp, bream, dace, eel, mandarin fish and other fish. The disease has an acute onset, many types of freshwater fish, a wide range of fish specifications, a wide epidemic area, and a long epidemic season, causing serious losses to the freshwater aquaculture industry. The pathogen is mainly Aeromonas ( Aeromonas ) of Aeromonas hydrophila ( A.hydrophila ) and Aeromonas temperatus ( A. sobria ), which widely exist in fres...

Claims

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Application Information

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IPC IPC(8): A61K39/02A61P31/04
Inventor 陶家发吴淑勤石存斌任燕潘厚军赖迎迢王庆刘礼辉
Owner PEARL RIVER FISHERY RES INST CHINESE ACAD OF FISHERY SCI
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