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A kind of dual inactivated vaccine of Aeromonas hydrophila and Aeromonas victoria and its preparation method

A technology of Aeromonas victoria and Aeromonas hydrophila, applied in the direction of antibacterial drugs, bacterial antigen components, etc., can solve the problems of unstable immune effect, different pathogenicity, and difficulty in wide application

Active Publication Date: 2018-06-19
XINXIANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are also some problems in these vaccines, such as unstable immune effect, poor safety, difficult preparation technology, high cost, lack of reasonable, effective and convenient administration methods, etc. Therefore, the wide application of these vaccines in actual production still exists. certain difficulty
[0004] Aeromonas hydrophila and Aeromonas victoria are heterologous, because there are differences in physiological and biochemical characteristics, serotypes, and genotypes between different strains, and their pathogenicity is also due to their differences in the expression of virulence factors. Different from each other, thus, limited in the popularization of vaccines

Method used

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  • A kind of dual inactivated vaccine of Aeromonas hydrophila and Aeromonas victoria and its preparation method
  • A kind of dual inactivated vaccine of Aeromonas hydrophila and Aeromonas victoria and its preparation method
  • A kind of dual inactivated vaccine of Aeromonas hydrophila and Aeromonas victoria and its preparation method

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Experimental program
Comparison scheme
Effect test

Embodiment

[0017] 1 material

[0018] 1.1 Strains

[0019] Aeromonas hydrophila and Aeromonas victoria strains were isolated from diseased fish suffering from bacterial septicemia in Xinxiang City, including 32 strains of Aeromonas hydrophila and 20 strains of Aeromonas vickeris, frozen Store dry.

[0020] 1.2 Experimental animals

[0021] Healthy crucian carp was purchased from a fishing ground in the suburbs of Xinxiang City. The weight of each fish was about 30g and the length was 12-15cm. Before use, domesticate them in the laboratory for 1 week, inflate them, control the water temperature at about 28°C, clean and change water, and feed them regularly.

[0022] 1.3 Medium

[0023] Preparation of LB nutrient broth: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, potassium dihydrogen phosphate 1.0g, distilled water 1000mL, mix well, heat to dissolve, adjust the pH value to 7.6, subpackage, and extinguish under high pressure at 112kPa bacteria for 15 minutes.

[0024] Prep...

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Abstract

The invention provides a double oral sustained-release microsphere vaccine of Aeromonas hydrophila and Aeromonas victorii and a preparation method thereof, belonging to the field of biopharmaceuticals. Sodium alginate was used as the wall material, and the inactivated whole bacteria of Aeromonas hydrophila and Aeromonas victoria were used as the core material. released microsphere vaccines. Using the Aeromonas hydrophila and Aeromonas victoria-calcium alginate microcapsule oral vaccine to immunize gibel crucian carp can significantly improve serum enzyme activity and leukocyte phagocytosis activity, and its relative protection rate against gibel carp is 46.7 %. The microcapsules are stable under simulated gastrointestinal conditions, showing good burst and sustained release properties; the bacterial antigenicity in the microcapsules remains good, and the vaccine safety and storage stability are good.

Description

technical field [0001] The invention relates to animal vaccine preparation technology, in particular to a dual inactivated vaccine of Aeromonas hydrophila and Aeromonas victorii and a preparation method thereof. The vaccine of the present invention is mainly suitable for freshwater fish. Background technique [0002] Aeromonas hydrophila and Aeromonas hydrophil (Aeromonas hydrophil) belong to Vibriaceae and Aeromonas genus, widely exist in fresh water, soil, sewage, sludge and feces, there are pathogenic strains and non-pathogenic strains Pathogenic strains. The infection spectrum of pathogenic strains is very wide, and can cause outbreaks of infections in soft-shelled turtles, shrimps, crabs, frogs, ducks, etc. Human infection with this strain can cause food poisoning and soft tissue injury infection, and it is the main pathogenic bacteria of freshwater fish. Bacterial septicemia caused by this bacterium is one of the acute infectious diseases that harm the most species o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/116A61K39/02A61P31/04
Inventor 关建义刘涌涛毛会丽贺文旭杨利敏符运栋
Owner XINXIANG MEDICAL UNIV
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