A novel bacteriostatic compound, a preparing method thereof and applications of the bacteriostatic compound in aquatic products

A technology for compounds and aquatic products, applied in the field of new antibacterial compounds, to achieve the effects of low side toxicity, rapid bactericidal effect and strong antibacterial activity

Inactive Publication Date: 2016-07-20
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The application number is 201210444272.1 (the application publication number is CN102964234A) discloses the application of a chalcone compound in the preservation of aquatic products. At present, there is no report

Method used

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  • A novel bacteriostatic compound, a preparing method thereof and applications of the bacteriostatic compound in aquatic products
  • A novel bacteriostatic compound, a preparing method thereof and applications of the bacteriostatic compound in aquatic products
  • A novel bacteriostatic compound, a preparing method thereof and applications of the bacteriostatic compound in aquatic products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: Preparation of novel bacteriostatic compound (Butein-0908)

[0019] Add 0.426g 2.8mmol 2,4-dihydroxyacetophenone 0.426g to 0.5g 2.8mmol compound I and mix well, add dropwise a 14% KOH solution prepared by 30mL absolute ethanol and 4.2g KOH under nitrogen protection, and react at room temperature 8h. After the reaction is finished, the reaction solution is poured into 30mL ice water, and the pH value is adjusted to neutrality with 3mol / L HCl, and the yellow solid is obtained by filtration, dried, and the thick product is recrystallized with 95% ethanol to obtain a yellow crystal, which is a novel bacteriostatic compound (Butein -0908).

[0020] Yield 81.6%, melting point 198.3°C. 1 HNMR (CDCl 3 )δ: 1 H-NMR (CCl 3 D) δ: 6.37-7.51 (m, 3H, -C 6 h 3 ), 6.51 (d, 1H, J=15Hz, =CH), 7.57 (d, 1H, J=15Hz, =CH), 7.62 (d, 1H, J=15Hz, =CH), 7.20-7.38 (m, 4H, -C 6 h 4 ), 8.09 (d, 1H, J=15Hz, =CH), 10.4 (s, 1H, -OH), 11.2 (s, 1H, -OH), 13.3 (s, 1H, -COOH); IR (K...

Embodiment 2

[0021] Embodiment 2: Butein-0908 antibacterial test in vitro

[0022] 2.1 Preparation of bacteria solution

[0023] Adopt beef extract peptone agar medium to carry out bacterial culture, adopt potato agar medium (PDA) to carry out mold culture: (1) liquid medium preparation: beef extract 3g, peptone 10g, sodium chloride 5g, distilled water 1000mL, 2% agar, The pH value is 7.0 to 7.2, heated and melted, packed in a triangular flask, sterilized at 121°C for 15 minutes, taken out and cooled for later use; (2) Preparation of test tube slope: inject about 5mL of beef extract peptone medium into the test tube, plug it with cotton Stopper, put it on an oblique after sterilization. Preparation of the test strains: inoculate the test bacteria strains on the inclined surface of the test tube, and cultivate them in a 35-37°C incubator for 18-24h; Soak in 0.5% tea polyphenols for 10 minutes, and soak in 0.75% normal saline as the control, and dry in an oven at 55°C.

[0024] 2.2 Prepar...

Embodiment 3

[0031] Example 3: In vitro antibacterial test of Butein-0908 on pathogenic bacteria in aquatic products

[0032] 3.1 Bacteria solution configuration

[0033] The preserved strains were first inoculated on ordinary nutrient agar plates, cultivated at 30°C for 24 hours, selected typical colonies and inoculated on beef extract peptone medium, cultivated overnight at 30°C, and diluted 100-fold with beef extract peptone medium as the test bacterial solution.

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Abstract

The invention relates to a novel bacteriostatic compound named as Butein-0908, a preparing method thereof and applications of the bacteriostatic compound in aquatic products. When the mass percentage is 0.06%, the bacteriostatic compound can inhibit staphylococcus aureus, bacillus subtilis, salmonella bacillus, bacillus proteus and escherichia coli, has strong inhibiting functions for common pathogenic bacteria of the aquatic products, has obvious and rapid sterilization functions for aeromonas hydrophila, aeromonas sobria and non-O1 group vibrio cholerae, and can kill all bacteria in 12 h. Accordingly, the bacteriostatic compound is wide in antibacterial spectrum, few in side and toxic effects and high in bacteriostatic activity, is especially suitable for the common pathogenic bacteria of the aquatic products, and can be widely used as an economical and environmental friendly bacteriostatic agent for aquatic product bacteriostasis.

Description

technical field [0001] The present invention relates to antibacterial compounds, in particular to a novel antibacterial compound Butein-0908 [3-(4-(3-(2,4-dihydroxyphenyl)-3-oxopropenyl)phenyl)acrylic acid] Its preparation method and application in aquatic products. Background technique [0002] Antibiotics currently used in aquatic feed and fish medicines have long half-lives and high residues, which reduce the quality of aquatic products on the market, especially chloramphenicol, oxytetracycline, tetracycline, neomycin sulfate and sulfonamides. The action and metabolism in animals take a long time, affect the physiological functions of animals, and cause adverse reactions such as allergies, urinary system and hematopoietic system dysfunction. In addition, due to the improper application of these additives or antibacterial and antiviral drugs in aquaculture, they will pollute the environment of the aquaculture waters, affect the growth of fish, cause the aquatic products t...

Claims

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Application Information

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IPC IPC(8): C07C59/90C07C51/353C07C51/373A61P31/04A61P31/10
CPCY02A50/30
Inventor 关丽萍金晴昊甄兴华赵水莲彭舟
Owner ZHEJIANG OCEAN UNIV
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