Porcine interferon gene family and application thereof in blue-ear porcine disease resistant medicament and vaccine adjuvant

Abstract

The invention belongs to the technical field of genetic engineering, and particularly relates to a porcine interferon family gene and application thereof in a blue-ear porcine disease resistant medicament and a vaccine adjuvant. The porcine interferon gene is taken from a home pig genome and has anti-virus effect, and the amino acid sequence of the porcine interferon gene is SEQ ID No. 1, SEQ ID No. 3, SEQ ID No. 5, SEQ ID No. 7 or SEQ ID No. 9; and the medicament and the vaccine adjuvant are a recombinant plasmid obtained by cloning the home pig interferon gene to an eukaryotic expression vector through molecular biology technology and a live bacterial vaccine obtained by transferring the plasmid to a low virulent strain of Salmonella choleraesuls. Animal experiments prove that the medicament and the adjuvant have good safety and remarkable effect. After a home pig is directly injected, the toxicity is removed on the same day, and the protective rate can reach 80 percent. When the adjuvant is used with a blue-ear disease inactivated vaccine and after the home pig is subjected one-time immune injection, the toxicity is removed in 28 days, and the protective rate can reach 80 percent.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and specifically relates to a new porcine interferon gene family and its application as anti-pig PRRS drug and vaccine adjuvant. Background technique [0002] There are several types of porcine type I interferon, and each type has a group of multi-gene families, which are cytokine families with antiviral effects. Under normal physiological conditions, they are not expressed or have a small amount of constitutive expression in animals. When the body is infected by a virus, they not only have antiviral effects, but also can up-regulate the body's immune response to clear the virus. Studies have shown that type I interferon can also activate B cells and help B cells to quickly produce antibodies against pathogens; promote the expression of MHC-I antigens in most cells, induce the proliferation of NK cells and T cells and increase their cytotoxic activity; Helper CD8 + The growth of me...

AI Technical Summary

Problems solved by technology

So far, various inactivated vaccines have been studied and prepared at home and abroad, but existing reports show that the immunogenicity of inactivated vaccines is poor, and the protective effect on immunized pigs is very low

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