Mutant strain of Brucella bacterin with weak poison, constructing method, and application
A technology of Brucella and attenuated vaccine, applied in bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of difficult diagnosis of brucellosis, inability to identify antibodies, interference with conventional serology and ELISA diagnosis, and achieve immune protection. Good effect, no increase in virulence, and broad application prospects
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[0024] Example 1. Brucella Ovis M 5 Construction of Vaccine Strain bp26 Gene Deletion Mutant and Evaluation of Its Virulence and Immune Protection
[0025] 1. Brucella sheep species M 5 Construction of bp26 gene deletion mutant strain of vaccine strain
[0026] The conditions for selecting the deletion target gene are: first, the gene deletion does not affect the replication of the Brucella vaccine mutant, and the virulence does not increase, but the immunity does not decrease; second, the deleted gene should encode an immunodominant antigen protein. According to the above conditions, wboA, bp26, omp31, P39 or pgm were selected as the deletion target genes for constructing Brucella attenuated vaccine mutant strains. Taking the deletion of bp26 as an example, see Figure 1 to construct Brucella Ovis M with the method of the present invention 5 The bp26 deletion mutant of the vaccine strain, the specific process includes the following steps:
[0027] 1. PCR primer design
[0028] Accor...
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