Mutant strain of Brucella bacterin with weak poison, constructing method, and application

A technology of Brucella and attenuated vaccine, applied in bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of difficult diagnosis of brucellosis, inability to identify antibodies, interference with conventional serology and ELISA diagnosis, and achieve immune protection. Good effect, no increase in virulence, and broad application prospects

A technology of Brucella and attenuated vaccine, applied in bacteria, antibacterial drugs, bacterial antigen components, etc., can solve the problems of difficult diagnosis of brucellosis, inability to identify antibodies, interference with conventional serology and ELISA diagnosis, and achieve immune protection. Good effect, no increase in virulence, and broad application prospects

CN101092605AInactive Publication Date: 2007-12-26MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Mutant strain of Brucella bacterin with weak poison, constructing method, and application

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Embodiment 1

[0024] Embodiment 1, Brucella sheep species M 5 Construction of vaccine strain bp26 gene deletion mutant and evaluation of its virulence and immune protection

[0025] 1. Brucella sheep species M 5 Construction of Vaccine Strain bp26 Gene Deletion Mutant

[0026] The conditions for selecting the deletion target gene: first, the deletion of the gene does not affect the replication of the Brucella vaccine mutant, and the virulence does not increase, but the immunity does not decrease; second, the deletion gene should encode an immunodominant antigen protein. According to the above conditions, wboA, bp26, omp31, P39 or pgm were selected as the deletion target gene for constructing the attenuated Brucella vaccine mutant strain. Taking the deletion of bp26 as an example, see Fig. 1 to construct Brucella sheep species M by the method of the present invention 5 The bp26 deletion mutant of the vaccine strain, the specific process includes the following steps:

[0027] 1. PCR primer...

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Abstract

This invention discloses attenuated Brucella mutant, its construction method and its application. The attenuated Brucella mutant is constructed by deleting bp26, wboA, omp31, and P39 or pgm gene from attenuated Brucella. It is proven by experiments that attenuated Brucella mutant has good immune protection effects and the same virulence as attenuated Brucella. Because certain antigen is delected in the attenuated Brucella mutant, vaccine immunity and natural infection can be differentiated according to the immunobiology of the deleted gene. The attenuated Brucella mutant can be used to manufacture preventative Brucella vaccine and marked attenuated vaccine, and prevent epidemic diseases caused by Brucella.

Description

technical field [0001] The invention relates to a vaccine mutant and its construction method and application, in particular to a Brucella attenuated vaccine mutant, its construction method and its application in preparing a Brucella preventive vaccine. Background technique [0002] Brucellosis (brucellosis) is a widespread and extremely harmful zoonotic disease caused by Brucella. Class II infectious diseases, and listed as the first class II infectious diseases recorded in the "Implementation Rules of the Regulations on Epidemic Prevention of Livestock and Poultry". So far, the disease has spread to five continents in the world, and there are more than 160 countries and regions in more than 200 countries and regions. Brucellosis is one of the most widespread and harmful zoonotic diseases in the world, seriously affecting people's health and the development of animal husbandry. When people suffer from this disease, the disease period can be as long as several months, sever...

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Application Information

Patent Timeline
26 Dec 2007
Publication
CN101092605A
IPC
C12N1/21; A61K39/10; A61P31/04
Inventors
王效义; 王棠