The invention discloses a food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array. The food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array comprises a substrate, and a pathogenic bacterium, a positive control and a negative control are fixed on the substrate. The pathogenic bacterium is one or more of staphylococcus aureus, hemorrhagic escherichia coli O157: H7, Listeria monocytogenes, salmonella cholerae suis, salmonella typhimurium, salmonella enteritidis, Shigella flexneri, Shigella sonnei, shigella dysenteriae, Shigella boydii, vibrio parahaemolyticus, enterobacter sakazakii, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, Salmonella paratyphi A and Salmonella paratyphi B. According to the food-borne pathogenic bacterium hybridoma cell rapid screening antigen macro array, multi-target screening can be carried out, dozens of hybridoma cells can be screened within 2.5 hours, the preparing period of food-borne pathogenic bacterium monoclonal antibody is greatly shortened, and the production cost is reduced.