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Application of oligonucleotide aptamer capable of identifying salmonella specifically

An oligonucleotide and Salmonella technology, applied in the fields of food and hygiene detection and biology, can solve the problems of long detection cycle, many false positives, low sensitivity, etc., and achieve the effect of short detection time, short development cycle and simple operation.

Inactive Publication Date: 2012-12-05
安徽出入境检验检疫局检验检疫技术中心
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Problems solved by technology

[0005] In order to solve the problems of long detection period, low sensitivity, and many false positives in the existing detection of Salmonella, the present invention provides an application of an oligonucleotide aptamer that specifically recognizes Salmonella

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  • Application of oligonucleotide aptamer capable of identifying salmonella specifically
  • Application of oligonucleotide aptamer capable of identifying salmonella specifically
  • Application of oligonucleotide aptamer capable of identifying salmonella specifically

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Embodiment Construction

[0015] The nucleotide sequence of the oligonucleotide aptamer that specifically recognizes Salmonella is as follows: 5'-GATCCGGGCCTCATGTCGAACACCCCCCAACTAAAACAACAAAACACCACCGCCATTGAGCGTTTATTCTGAGCTCCCA-3'; the oligonucleotide aptamer that specifically recognizes Salmonella is a single-stranded DNA with a length of 78 bases. The invention obtains the oligonucleotide aptamer with high affinity and specific recognition of Salmonella through the screening process of SELEX technology, and is used for rapid and accurate detection of Salmonella O8.

[0016] The oligonucleotide aptamer specific to Salmonella O8 was obtained by SELEX screening, and the recognition and identification of the oligonucleotide aptamer with the highest affinity for Salmonella O8 was further described.

[0017] 1. The random single-stranded DNA library and primers were synthesized by Shanghai Bioengineering Co., Ltd.

[0018] Random ssDNA library: 5'-GGGAGCTCAGAATAAACGCTCAA-

[0019] 35nt-TTCGACATGAGGCCCGGATC...

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Abstract

The invention relates to application of an oligonucleotide aptamer capable of identifying salmonella specifically. The oligonucleotide aptamer is a single-stranded deoxyribonucleic acid (DNA) which has 78 bases in length, and is applied to the detection of the salmonella 08. Food-borne salmonella 08 is used as a target molecule, and escherichia coli 086: K61 and salmonella choleraesuis are used as reverse screening bacteria, so that a specific aptamer of the salmonella 08 is obtained. The method has the advantages of short detection time, short development period, stable quality, simplicity of operation and the like, and is widely applied to the detection of food and sanitary safety.

Description

field of invention [0001] The invention belongs to the technical field of biology, food and sanitation detection, and specifically relates to the use of an oligonucleotide aptamer that specifically recognizes Salmonella (serum type O8, hereinafter referred to as Salmonella O8). Background technique [0002] At present, the detection methods commonly used in the detection of Salmonella in food at home and abroad have their own advantages and disadvantages due to their different detection methods and identification objects. The traditional method of detecting Salmonella needs to be isolated first, then cultivated, and then identified by the classic method. Time-consuming and insensitive are common problems of these methods. The immunological method is simple, convenient, rapid, and has good specificity, but there are still shortcomings such as serious cross-reaction, many false positives, and low sensitivity. Although the polymerase chain reaction (PCR) technology has the cha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/10C12N15/115G01N21/64
CPCY02A50/30
Inventor 余晓峰张萍宗凯孙娟娟李云飞陈雪娇郑海松
Owner 安徽出入境检验检疫局检验检疫技术中心
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