77 results about "Pre enrichment" patented technology

The invention discloses a lead ion specific detection sensor and a preparation method and a using method thereof. The sensor comprises a three-electrode system, wherein a glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as a working electrode of the system, a platinum sheet is used as a counter electrode, and a calomel electrode is used as a reference electrode. When in detection, in the three-electrode system in which the glassy carbon electrode for depositing graphite, chitosan and Au nanoparticles is used as the working electrode, the platinum sheet is used as the counter electrode and the calomel electrode is used as the reference electrode, an electrochemical workstation is used for performing constant-potential lead ion pre-enrichment; and after the enrichment is finished, the lead ion is detected by a differential pulse working mode. Through the detection sensor, simple and quick specific detection on the lead ion is realized; the lead ion with concentration as low as 0.01ug/L can be detected by the method; meanwhile, the lead ion sensor is simple to prepare, has stable performance and can be repeatedly used; and the sample detection time is short, and the operation is convenient.

The invention relates to the technical field of ore separation, in particular to a new efficient separation process of low-grade copper nickel sulfide ore. The process comprises the following steps of: grinding the low-grade copper nickel sulfide ore, and performing magnetic separation and pre-enrichment; performing ore grinding flotation on the magnetic separation ore concentrate after the magnetic separation and the pre-enrichment, and adding 25 to 100g/t of ethyl xanthogenate or butyl xanthogenate or dithiophosphate BA to obtain high-grade copper nickel ore concentrate; grading the magnetic separation tailings after the magnetic separation and the pre-enrichment, and performing ore grinding flotation on the coarse product after grading, and adding 25 to 100g/t of ethyl xanthogenate or butyl xanthogenate or dithiophosphate BA to obtain a qualified ore concentrate product; and performing ore grinding flotation on the fine product after grading, adding 25 to 100g/t of ethyl xanthogenate or butyl xanthogenate or dithiophosphate BA, and adding a hyper-dispersant to obtain a qualified ore concentrate product, wherein the nickel grade of the obtained qualified ore concentrate product is 5 to 10 percent.

The present invention relates to a protein pretreatment and polypeptide high-pH reverse-phase gradation-integrated proteome reactor and the application thereof. The proteome reactor comprises a pipette gun head (1), a strong ion-exchange resin filler (2) and a solid-phase extraction membrane (3). The solid-phase extraction membrane (3) is filled in the lower end of the pipette gun head (1) and is positioned above the solid-phase extraction membrane (3). The proteome reactor provided in the invention integrates the operations of protein pre-enrichment, reduction, alkylation, enzymatic hydrolysis, polypeptide desalination, elution and high-pH reverse-phase gradation, thus being applicable to the large-scale protein identification and the quantitative proteomics for a small number of cells or tissue samples. In addition, the proteome reactor technology can realize the automated liquid handling platform-based automatic operation, such as the Agilent bravo platform-based automatic operation. Therefore, the sample processing throughput and the accuracy of the quantitative analysis are improved.

The invention relates to the field of safety monitoring of food microorganisms, and discloses a salmonella characteristic chromogenic liquid nutrient medium, a preparation method thereof and a rapid detection method of salmonella. The salmonella characteristic chromogenic liquid nutrient medium comprises the following main components: tryptone, yeast powder, sodium chloride, lithium chloride, sodium deoxycholate, dipotassium phosphate, combined inhibitor, combined accelerator, characteristic enzymolysis substrate and cosolvent. The rapid detection method disclosed by the invention comprises two steps, i.e. pre-enrichment culture and chromogenic identification, and is characterized in that salmonella characteristic enzyme hydrolyzes corresponding substrates to result in that the culture medium is purple, thus rapidly judging the existence of salmonella; and the addition of the accelerator contributes to recovering damaged cells of salmonella and promoting growth of salmonella; and the added inhibitor can selectively inhibit the growth of other competitors so as to reduce the interference on detection by the competitors. The detection method disclosed by the invention has the advantages of short detection period, strong specificity and high accuracy, is simple to operate and is suitable for large-throughput detection of salmonella in food.

The invention discloses an acrylic acid pyridine chelating resin as well as a preparation method and application thereof, and belongs to the field of modified resin synthesis. A basic structure unit of the resin is as shown in the specification, wherein x represents polyamines with different chain lengths; resin microspheres are faint yellow; the particle sizes are 0.15-1.20mm; and the functional group content is 1.08-3.01mmol/g. The chelating resin disclosed by the invention is wide in preparation material source, low in cost, and simple in operation step. Due to the hydrophilcity of a polyacrylic resin skeleton, the adsorption rate on heavy metal is high. In addition, due to high modification quantity of the resin functional group, the adsorption quantity on heavy metal is large. The acrylic acid pyridine chelating resin disclosed by the invention can be applied to the fields such as mining and metallurgy production, mother liquid purification, removal of heavy metal in a high-acid and high-salt complicated system and separation and pre-enrichment of the heavy metal in environment analysis; the technical problems of small adsorption amount, low adsorption rate, large in required resin amount in engineering, long operation time and the like when the heavy metal is selectively adsorbed are solved; and the economic benefits and the environment benefits are significant.

The invention provides a method for preparing capillary column of titanium dioxide coating by using liquid phase deposition method; fluotitanic acid ammonium is taken as raw material and a film layer of the titanium dioxide is deposited on inner wall of the quartz capillary through the liquid phase deposition method. The novel method is used for preparing the capillary column of the titanium dioxide coating. The invention is simple in method, and the prepared capillary column of the titanium dioxide coating can be successfully used in separation and pre-enrichment of biological samples; the invention can also used for preparing the capillary column of other inorganic oxide coating.

The present invention relates to the application of beta-cyclodextrin bonded silica gel as solid-phase extraction adsorption medium. Said beta-cyclodextrin bonded silica gel features simple synthesis process and cheap raw material, when it is used for making solid-phase extraction, it features large penetration volume, high adsorption quantity, high selectivity, and can be regenerated and reused. It can be used for making selective extraction of nitrophenol compound in water sample, also can be used for pre-enrichment of medicine molecules in biological sample.

The invention relates to the field of comprehensive utilization of resources, and in particular relates to a comprehensive utilization method for iron and steel smelting dust washing wastewater. The method comprises the following steps: a) adding a precipitating agent and a flocculating agent into the iron and steel smelting dust washing wastewater to form a suspension; b) performing solid-liquid separation on the suspension, and collecting a solid phase and a liquid phase respectively; c) delivering the liquid phase to a reverse osmosis pre-enrichment system to complete pre-enrichment of alkali metals and chlorine ions and then obtain low salt water and a concentrated salt solution for washing smelting dust; and d) performing freeze drying on the concentrated salt solution to obtain a potassium salt and fresh water. The method realizes comprehensive utilization of the iron and steel smelting dust washing wastewater; the alkali metals and the chlorine ions can be pre-enriched through the reverse osmosis pre-enrichment system and freeze-dried, so that the energy consumption is reduced greatly compared with high salt water evaporation; meanwhile, the pre-enrichment manner has a relatively wide application prospect in the high salt wastewater treatment; the whole process is environment-friendly, economic and efficient.

The invention discloses a specific antibody of a campylobacter jejuni specific multi-epitope artificial polypeptide and coated immunomagnetic beads and an application thereof. The amino acid sequence of the artificial polypeptide prepared after tandem expression of a campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.1. A nucleotide sequence for encoding the campylobacter jejuni specific surface protein multi-antigen epitope is SEQ ID No.2. A kit for enriching campylobacter jejuni comprises immunomagnetic beads containing a specific antibody coated with the campylobacter jejuni specific multi-epitope artificial polypeptide. By applying the immunomagnetic beads or the kit, campylobacter jejuni can be enriched from a sample pre-enrichment liquid, and the sensitivity and detectable rate of subsequent separate culturing or PCR (Polymerase Chain Reaction) detection are increased greatly; and the specific antibody has the advantages of high specificity, high sensitivity, easiness, convenience, rapidness and suitability for large-scale sample detection.

The invention discloses a modified silica nanoporous membrane modified electrode, a preparation method and an application thereof, and belongs to the technical field of novel materials. The preparation method comprises the following steps of: (1) preparing a silica nanoporous membrane having an open pore structure on an electrode substrate; and (2) inserting graphene quantum dots into pores to modify the silica nanopores to prepare the modified silica nanoporous membrane modified electrode by using a electrophoresis method, an electrostatic adsorption method, or a covalent immobilization method. The modified electrode, the preparation method and the application thereof insert small-sized GQDs as a functional block into pores to modify a VMSF electrode to prepare a variety of sensing interfaces. The electrode can realize a pre-enrichment effect on an object to be detected due to the electronegativity of the pores, and the coordination effect on metal ions and the pi-pi stacking action on the dopamine of the graphene quantum dots, so that the detection property of the object to be detected is remarkably improved. The great application prospects are achieved in direct and high-sensitivity electrochemical detection of multiple types of active compositions in complex samples combined with the anti-staining/anti-interference ability of the VMSF.

The invention relates to hollow fibrous membrane-based liquid-phase micro-extraction technology under an electric field enhancement condition and a device for the same. The method comprises the following steps of: applying an appropriate direct-current electric field between sample solution 5 and an extraction phase 7; and using the adjustable electric filed to enhance an orientated mass transferprocess of an object to be analyzed between two phases to accelerate the balance of components to be analyzed between the two phases; therefore, the technology is novel liquid-phase micro-extraction technology. Compared with a conventional liquid-phase micro-extraction method, the technology fully embodies the characteristics of rapidness and high efficiency on the premise of having all the advantages of the conventional hollow fibrous membrane-based liquid-phase micro-extraction technology. The technology is pre-treatment and pre-enrichment technology for various analysis measures as well asa method for measuring a liquid-phase balancing parameter, so that the technology can be widely applied in various fields such as environmental engineering, medicament research and development, food safety and the like, and has high practical value.

Provided is an ammonia-free mining method for rare earth ore in south China. Magnesium ions and sodium ions are combined to serve as an ore leaching agent, and meanwhile magnesium salt serves as an enrichment agent. The ammonia-free mining method for rare earth ore in the south China includes the steps of leaching of rare earth ore, collection of rare earth mother liquor, pre-enrichment of rare earth, dissolution of rare earth hydroxide, rare earth sulfate double salt precipitation and rare earth sulfate double salt conversion. According to the ammonia-free mining method for rare earth ore in the south China, the ore leaching agent and precipitant which have no ammonia nitrogen and are relatively friendly to water are combined with the in-situ leaching mode, rare earth compounds meeting the use demands of a rare earth separation plant are generated through rare earth double salt precipitation, the production cost is low, and environmental pollution is greatly reduced.

The present invention provides a process whereby pre-enrichment of water streams using a hydrogen source and a catalytic isotope exchange method at one or more remote sites to supply water with augmented deuterium concentration to a central heavy water. This central heavy water plant could be a Combined Electrolysis and Catalytic Exchange (“CECE”) heavy water production plant or a Girdler Sulfide heavy water plant. The deuterium content of water at the remote sites is increased and provides water stream(s) with augmented deuterium concentration to feed to the central heavy water production plant. This could be a first stage of the central CECE deuterium enrichment plant, increasing its capacity for heavy water production approximately in the ratio of its enrichment above natural deuterium concentrations. By relatively simple utilization of available deuterium enrichment capacity at the remote sites, advantages are achieved from a larger scale of heavy water production at the central production plant. The invention further provides systems and methods for adapting chlorate and chlorine dioxide systems which produce hydrogen to additionally produce deuterium-enriched water.

The invention discloses a quantiative detection method of escherichia coli in soil and an assay kit thereof. On the basis of taking related researches as reference, the method performs quantitative detection on pathogenic microorganism in the soil by adopting a method of combining MPN (most probable number) with PCR (polymerase chain reaction). By utilizing advantages of quickness and accuracy ofthe PCR method, biochemical identification, serological identification and other steps of positive results in the traditional MPN method are substituted, so that complex steps in a national standard method are reduced, the detection efficiency is improved, and a pre-enrichment step in the national standard method is adopted at the same time to improve the accuracy of detection targeting pathogenic bacteria; and due to the complexity of soil, the soil contains massive microorganisms, as well as various pathogenic microorganisms of different items, so that the targeting pathogenic bacteria is selectively cultured to improve the detection efficiency in order to quantitatively detect the targeting pathogenic bacteria better and more accurately. By virtue of creative effort of the inventor andscientific test verification, the sensitivity of the escherichia coli quantitative detection method in an experiment of adding pure bacteria in the soil is 25 per gram of soil.

The invention relates to an ultrasonic reinforced hollow fiber membrane liquid-phase micro extraction technique and an ultrasonic reinforced hollow fiber membrane liquid-phase micro extraction device. In the technique, proper ultrasonic is applied between sample solution 2 and an extraction phase 4, and the mass transfer process of an analyzed component in each phase is reinforced by using the ultrasonic with adjustable frequency and power so as to quicken the balance of the analyzed component between the phases; and the technique is a novel liquid-phase micro extraction technique. Compared with the conventional liquid-phase micro extraction method, the technique fully reflects the characteristics of quickness and high efficiency on the premise of keeping all advantages of the traditional hollow fiber membrane-based liquid-phase micro extraction technique. The technique belongs to a pretreatment and pre-enrichment technique of multiple analysis means, is a method for measuring liquid phase balance parameters, can be widely applied in many fields of environmental engineering, medicament research and development, food safety and the like, and has strong practical value.

The invention relates to a method for quantitatively analyzing tyrosine-phosphorylated proteins. The method is characterized in that the dynamic change curve of the tyrosine phosphorylation level of asample to be tested is obtained based on an ELISA technology with a modified SH2 over parent as a first antibody, a standard curve is obtained with tyrosine-phosphorylated standard proteins as a reference sample in the detection process, and the quantitative analysis of the tyrosine phosphorylation level of the sample to be tested is realized with the standard curve as a reference. The method hasthe advantages of low cost, no sample pre-enrichment, good specificity and low detection limit. The ELISA technology is carried out in a well plate, and an apparatus is simple. The SH2 over parent adopted in the invention can be used to obtain a lot of proteins with a stable structure through bacterium expression and purification, so the experimental cost is greatly saved, the background is clear, and reconstruction is easy. The method has the same high affinity and high specificity to the tyrosine-phosphorylated proteins as antibody, has a broad spectrum, and can successfully realize the quantitative analysis of the tyrosine phosphorylation level of multiple complex samples.

The invention discloses an amidoxime-group pyridine chelating resin and a preparation method thereof. A structure unit of the amidoxime-group pyridine chelating resin is shown in the attached figure.The amidoxime-group pyridine chelating resin has the advantages that the preparation is simple, the cost is low, and the suitability to different heavy metal water bodies is strong; the oximido contains oxygen atoms and can be effectively bonded with the soft metal ions, the nitrogen atom of pyridine is difficult to protonize, and the pyridine can form feedback phi bond with the heavy metal ion, so that the removal ability on the heavy metal ions is still higher under the condition of higher acidity; the amidoxime-group pyridine chelating resin can be applied to the fields of removal of heavymetals in complicated water bodies, separation and pre-enrichment of interface heavy metals and soft metals in environment analysis, and the like; the economic benefits and environment benefits are obvious.

The invention discloses a pre-enrichment heavy liquid for extracting aluminum, lithium and gallium from fly ash and a pretreatment method, and relates to the technical field of resource utilization ofsolid waste fly ash. The pre-enrichment heavy liquid comprises bromoform and ethanol, and the density of the prepared heavy liquid is 1.8-2.4g/cm3. The pretreatment method comprises the processes ofheavy liquid preparation, centrifugal separation, filtration, atmospheric distillation, heavy liquid recovery and fly ash drying. The invention provides the method that fly ash is subjected to floating-sinking pretreatment and the fly ash is divided into two density grades. The selected heavy liquid is easy to prepare and can be recycled, the cost is saved without causing pollution to surroundingenvironment, and the content of aluminum, lithium and gallium in the high-density fly ash obtained by sorting is greatly increased; and at the same time, the treatment amount in the extraction processis reduced, the reasonable and efficient utilization of the fly ash can be realized, the method can be used for the pretreatment process of the fly ash when aluminum, lithium and gallium are extracted from the fly ash, and the effective enrichment of aluminum, lithium and gallium elements can be carried out.

The invention provides a high-sensitivity online device for detecting HCN in expiratory air, and an application thereof. The high-sensitivity online analysis system comprises an expiratory air sampling device, an expiratory air dehumidifying device, an air supply device and an HCN high-sensitivity detecting device. The application of the high-sensitivity online analysis system is to use the abovehigh-sensitivity online analysis system to directly convey human body expiratory air with a certain flow rate into the HCN high-sensitivity detecting device for analysis after the dehumidifying treatment of the dehumidifying device. HCN gases with different concentrations can be obtained by changing the flow rate of the human body expiratory air and the pumping speed of a pump, so as to obtain HCNfeature ion signal responses of different intensities. The human body expiratory air directly enters the HCN high-sensitivity detecting device without offline pre-enrichment, chemical derivation or other pretreatment steps, so that the operation is simple, the analysis is fast and accurate, the detection sensitivity is high, and the HCN of the sub-ppbv order can be detected.

The invention discloses a palladium solid phase extraction column and a using method thereof. According to the palladium solid phase extraction column disclosed by the invention, magnesium titanate is used as a matrix; dimethylglyoxime with a color rendering function is modified and loaded on the matrix; and a prepared palladium solid phase extracting agent with the adsorbing, color rendering and indicating functions is filled in a colorless and transparent column pipe to obtain the solid phase extraction column with the color rendering and indicating functions; and the solid phase extraction column is used for qualitative analysis and pre-enrichment and separation of palladium ions in a water sample in the analyzed and monitored field and can also be used for separation, enrichment and recovery of palladium in the metallurgy field. The palladium solid phase extraction column with the color rendering and indicating functions, which is disclosed by the invention, can be used for conveniently and qualitatively judging the palladium in solution directly by color change; in the process of separating, enriching and recovering the palladium, the extraction state and the use condition of the column can be judged by color; and the column has good selectivity, is stable, has strong enrichment and separation capacity and is convenient to use.

The present invention relates to concentrating disease causing agents, such as foodborne pathogens, from complex media to expedite their detection. In particular, the present invention relates to a method to pre-concentrate pathogens rapidly, thereby enabling earlier detection times. Primarily, the present invention utilizes an approach that can concentrate the pathogens by flowing a sample through immuno-capturing tubes (“entrapment chamber” or “chamber”) during an early pre-enrichment period. Also, the invention relates to using binding materials to trap disease causing agent that is desired to be removed from the complex media such as the blood of a patient. It also related to using lights of specific wavelength to inactivate pathogens. The light is used to activate reactive oxygen species using a photo-sensitizer or directly kill the pathogen using light of wavelength between 100 nm and 450 nm.

The invention discloses a method for extracting liquid beta-amylase from bran serving as a raw material. The method comprises the steps of extraction, primary filtering, pre-enrichment, secondary filtering, ultrafiltration and concentration, mixing, enzyme activity adjustment and the like. The method can effectively increase the beta-amylase content of filtrate before ultrafiltration and concentration, reduce enzyme loss in the membrane filtration process and improve recovery rate; and filter residues can be air-dried or dried in the sun to be used as a feed, so the production cost is reduced and a circular economic production mode is realized.

The invention relates to a lecithin/acetate composite enrichment material with a chitosan/acetate surface coating. The lecithin/acetate composite enrichment material comprises a lecithin/acetate composite substrate and the chitosan/acetate surface coating decorated on the surface of the composite substrate, wherein the lecithin/acetate composite substrate comprises 5 to 20% by weight of lecithin and 80 to 95% by weight of acetate; and the chitosan/acetate surface coating comprises 30 to 50% by weight of chitosan and 50 to 70% by weight of acetate. In the invention, the composite enrichment material is prepared to a ball-shaped, fibre-shaped and flat film or a pipe-shaped film. The composite enrichment material is mainly applied to pre-enrichment and removal of hydrophobic toxic organic pollutants in water body, in particular the pre-enrichment and the removal of hydrophobic persistent organic pollutants proposed in the Stockholm convention.