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Method for extracting liquid beta-amylase from bran

A technology of amylase and bran, applied in the direction of hydrolytic enzymes, etc., can solve the problems of low content of β-amylase, low recovery rate, and large loss of enzyme activity

Inactive Publication Date: 2011-02-23
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The "Preparation Method of Liquid Beta-amylase Preparation" (Patent Publication No. CN 101323849A) of Wuxi Saide Biological Engineering Co., Ltd. proposes a method for extracting beta-amylase from bran, but it has the following problems: ultrafiltration The content of β-amylase in the filtrate before concentration is not high, the loss of enzyme activity is large during concentration, and the recovery rate is low

Method used

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  • Method for extracting liquid beta-amylase from bran
  • Method for extracting liquid beta-amylase from bran

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] (I) Leaching

[0052] (A) preparation of extraction water: add potassium sorbate respectively in water according to mass volume ratio: 0.3% (w / v), Na 2 S 2 o 5 : 0.5% (w / v), NaHSO 3 : 1.0% (w / v) and Na 2 S 2 o 3 : 0.1% (w / v); adjust the acidity of the solution to 5.0 and the temperature to 50°C respectively;

[0053] (B) 200kg of bran is added to the leaching water prepared in 1600L step (A), and leaching for 1.0 hour;

[0054] (II) Primary filtration

[0055] After leaching, pass through a 100-mesh double-layer filter cloth to collect the primary filtrate; the filter residue is rinsed with 400L of leaching water and combined with the primary filtrate to obtain a combined solution. The volume of the combined solution is about 1600L;

[0056] (III) Pre-enrichment

[0057] (A) 200Kg bran is added in the combined liquid that step (II) obtains, leaching 1.0 hour;

[0058] (B) After repeating step (II) and (A) of step (III) 4 times, pass through 100 mesh double-lay...

Embodiment 2

[0068] (I) Leaching

[0069] (A) preparation of extraction water: add potassium sorbate respectively in water according to mass volume ratio: 0.3% (w / v), Na 2 S 2 o 5 : 1.0% (w / v), NaHSO 3 : 0.3% (w / v) and Na 2 S 2 o 3 : 0.2% (w / v); adjust the acidity of the solution to 5.0 and the temperature to 50°C respectively;

[0070] (B) Add 1000kg bran to the leaching water prepared in 10000L step (A), and leaching for 1.0 hour;

[0071] (II) Primary filtration

[0072] After leaching, pass through a 100-mesh double-layer filter cloth to collect the primary filtrate; the filter residue is rinsed with 2500L of leaching water and combined with the primary filtrate to obtain a combined solution. The volume of the combined solution is about 10000L;

[0073] (III) Pre-enrichment

[0074] (A) 1000Kg bran is added in the combined liquid that step (II) obtains, leaching 1.0 hour;

[0075] (B) After repeating step (II) and (A) of step (III) 7 times, pass through 100 mesh double-layer ...

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Abstract

The invention discloses a method for extracting liquid beta-amylase from bran serving as a raw material. The method comprises the steps of extraction, primary filtering, pre-enrichment, secondary filtering, ultrafiltration and concentration, mixing, enzyme activity adjustment and the like. The method can effectively increase the beta-amylase content of filtrate before ultrafiltration and concentration, reduce enzyme loss in the membrane filtration process and improve recovery rate; and filter residues can be air-dried or dried in the sun to be used as a feed, so the production cost is reduced and a circular economic production mode is realized.

Description

technical field [0001] The invention relates to a method for extracting liquid beta-amylase, which belongs to the field of food. Background technique [0002] β-amylase (1,4-α-D-glucan maltohydrolase, EC 3.2.1.2) is an exo-amylase, which acts on starch from the non-reducing end of the α-1,4 glycosidic bond. Cut off one maltose unit at a time to generate maltose and macromolecular β-limited dextrin, and its hydrolysis rate to soluble starch is generally about 50-60%. [0003] β-amylase widely exists in barley, wheat, soybean, sweet potato and other higher plants and some microorganisms. The content of barley and wheat is high, and the content of sweet potato is only about 1 / 2 of that of barley. Because the β-amylase produced by microbial fermentation has low activity and high cost, and it is difficult to produce β-amylase from bacteria on a large scale. For this reason, all beta-amylases used industrially are of plant origin. [0004] The characteristic that β-amylase can...

Claims

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Application Information

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IPC IPC(8): C12N9/24
Inventor 张剑李睿张开诚杨明张顺喜张选明
Owner WUHAN POLYTECHNIC UNIVERSITY
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