44 results about "Amylase preparation" patented technology

The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature amylase, which has the preservation number of CCTCC NO: M207193, and is obtained through compound mutation of the prior Bacillus licheniformis system, preservation, rejuvenation and a selective breeding method; and then the Bacillus licheniformis is optimized by a culture medium formulation and optimized by fermenting technological conditions so as to establish the method for producing the acid-proof high-temperature amylase, wherein the method comprises the following steps: air culturing the Bacillus licheniformis for about 72 hours at a temperature of 37 DEG C to reach an enzyme generating peak; and obtaining concentrated enzyme fluid through flocculating treatment, pressure filtration and ultra-filtration concentration or obtaining different amylase preparations such as micro pills, granules or powder through other post treatment modes. The produced high-temperature amylase has good acid-proof activity, and can be widely applied in the food and medicament industries, particularly the industries such as alcoholic fermentation, monosodium glutamate, antibiotics, textile and papermaking.

The invention discloses a method for preparing high-temperature alpha-amylase by a heat resistant high-temperature laceyella sacchari RHA1 virus strain and a purification method thereof. The high-temperature laceyella sacchari RHA1 virus strain is white and transparent, has corrugations, dry surfaces, different positive colors and negative colors, ball shapes, laceyella sacchari gram-positive bacterium, no flagella or no pilus; and a large number of mycelium is produced when culturing liquid. The method for preparing the amylase by the high-temperature laceyella sacchari RHA1 comprises the following steps: carrying out ultrafilter concentration and ammonium sulfate precipitation on a fermentation supernatant through an ultrafilter envelope of 1KD to obtain crude enzyme liquid, and filtering by Superdex 75 10/300 gelatin to obtain pure heat resistant alpha-amylase. The molecular weight of the alpha-amylase obtained in the invention is only 11.9 KDa, and the alpha-amylase is a monomer protein and is an amylase with the minimal molecular weight reported recently. The alpha-amylase hydrolyzes amylose and soluble starch to obtain maltose and maltotriose, can resist high temperature and has wide action temperature range and application values in food processing, medicines, detergents and the like.

The invention discloses a preparing method of thermophilic alpha-amylase (PFA), which comprises the following steps: heating and dissolving recombinant protein inclusion body under the condition of alkalinity; diluting; compositing; separating and purifying through column layer. This method possesses high reclaiming ratio and high alpha-amylase activity.

The invention relates to a separation and purification method for amylase in a plant, and particularly relates to a method for industrially producing high-activity beta-amylase. The preparation method for the beta-amylase disclosed by the invention comprises the following steps of: collecting separation water; pre-treating; precipitating and dissolving out the beta-amylase; forming a composite precipitate from polyacrylic acid and the beta-amylase in the solution; dissolving the composite precipitate; and adjusting the pH to be greater than or equal to 6.0, and dissolving out the beta-amylase. The invention provides a preparation method for beta-amylase, which is less in resource consumption, high in yield (of about 75%), and high in activity (of 700000-900000 u/ml generally); and the obtained product is good in tolerance to temperature and pH value, and low in cost in case of being applied to maltose production.

The invention discloses raw-starch amylase producing penicillium and a raw-starch amylase preparation produced thereby. The invention provides penicillium sp. GXU20 CGMCC No.3690. The invention also provides the raw-starch amylase preparation produced by fermentation of penicillium GXU20. The penicillium GXU20 and the raw-starch amylase preparation both can be used in alcohol production for avoiding thermophilic digestion of raw materials and saving a large amount of energy; the addition of alpha-amylase is avoided, and cost is saved; and saccharification and fermentation are performed in the same container, and equipment cost is reduced. The raw-starch amylase producing penicillium and the raw-starch amylase preparation produced thereby are expected to solve the problem of high alcohol production cost caused by the thermophilic digestion of starch.

The invention discloses a preparation method for immobilized alpha-amylase with graphene/titanium oxide composite porous microspheres as a carrier. The preparation method particularly includes the steps of: 1) preparing the graphene/titanium oxide composite porous microspheres; 2) preparing an alpha-amylase solution, mixing and stirring the graphene/titanium oxide composite porous microspheres with the alpha-amylase solution for 1-2 h, and performing ultrasonic treatment for 1-4 h at the power of 300 W, and filtering the liquid to obtain a solid; 3) washing the solid with a buffer solution composed of citric acid and sodium citrate, and drying the solid to obtain the immobilized alpha-amylase. The immobilized alpha-amylase is low in deactivation, has excellent repeatability, and is good in thermo-stability.

The invention discloses a preparation method of amylase. With barley malt as a raw material, the method comprises the following steps: grinding of barley malt, extraction of amylase, preliminary filtration of the extract liquid, enrichment of the amylase, precipitation of the extract liquid, fine filtration of the extract liquid, ultrafiltration concentration, blending, secondary fine filtration and aseptic packaging. By adopting the method disclosed by the invention, the enzyme activity of the prepared amylase is not lower than 700,000U/ml, the purity is high, the stability is good, and the application prospect is broad.

A wide-temperature amylase production method belongs to the technical field of bioengineering. A strain used in the method is BL-mut08 (bacillus licheniformis-mut08), which is preserved in CCTCC (china center for type culture collection) and encoded as CCTCC NO.M207193, and is disclosed in China patent 200810020635.2. By means of compound mutation, preservation, rejuvenation and breeding of an original BL system and optimizing a medium formula and fermentation process conditions, the method of producing wide-temperature amylase is established, and the amylase produced has a wide range of an action temperature and high heat resistance. The strain reaches a peak of amylase production after being cultured for 72 hours at 37 DEG C. fermentation liquor is flocculated, press-filtered, super-filtered and concentrated to obtain the concentrated liquid, and different amylase preparations, such as pellets, particles or powder, are obtained by other post-processing methods. The amylase preparation is widely applicable to food and medicine industries, especially textile industry, paper industry and industries of alcoholic fermentation, monosodium glutamate, antibiotics and the like.

The invention discloses a method for making fermented special sweet wine by using indica rice. The method comprises the following steps: (1) treating indica rice; (2) spraying an amylase preparation;(3) performing mixing with koji; (4) performing saccharifying and fermenting; (5) performing fermenting at low temperature; and (6) performing pasteurization. According to the invention, indica rice is used as a raw material for making the sweet wine, then rhizopus and sacchromycopsis fibuligera are used for preparing the compound strain sweet wine koji, and the compound strain sweet wine koji isused for fermentation. According to detection, the content of beta-phenylethanol in the obtained sweet wine is obviously improved, and generation of bad flavors such as bitterness and the like in a fermentation process is reduced by adopting low-temperature fermentation. In addition, maltose amylase is added in the early stage of fermentation of the indica rice sweet wine to solve the problem of regeneration of indica rice, and the finally made fermented special sweet wine has the characteristics of outstanding fragrance and clear color. Compared with conventional glutinous rice sweet wine made by rhizopus yeast, the fermented special sweet wine has the advantages that operation is simple, raw material cost can be greatly reduced by making sweet wine by using indica rice, quality of the sweet wine is better, and thus the method has a good application prospect.

The invention provides a preparation method for liquid composite alpha-amylase. Through the combination of alpha-amylase and multiple chemicals with special structures, special linking action is generated between the chemicals and the macromolecules of amylase protein so as to enhance structural stability and adhesion to substrate, thereby realizing the large promotion of the catalytic activity of the amylase. The method has the advantages of simple preparation technology, convenience in operation, no need of special equipment, little investment, little contamination, easy control of technological parameters; compared with traditional commercial amylase, the decomposition efficiency of the prepared product for starch can increase by 100% to 200%, and the cost can be greatly lowered at the same time.

The invention discloses a method for preparing modified alpha-amylase. The method includes the steps that chitosan is oxidized through an oxidizing agent to obtain oxidized chitosan; the oxidized chitosan and alpha-amylase are reacted to obtain a cross-linking reaction mixture; a reducing agent is added into the cross-linking reaction mixture, reacting is carried out, and the modified alpha-amylase is obtained. By means of the mode, the environmental suitability and the stability of the alpha-amylase can be improved.

The invention relates to a method for producing coenzymes Q10 via the fermentation of genetic recombinant bacteria. According to the method, NAD (Nicotinamide Adenine Dinucleotide) kinase genes are transferred into strains of reducing coenzymes Q10 with high-efficiency expression. The culture condition of optimizing the recombinant bacteria comprises (1) cant activation; (2) seed culture; and (3) fermentation culture. The recombinant bacteria constructed by a genetic engineering means are strong in stability; compared with original strains, the coenzymes Q10 of the recombinant bacteria has the advantages that the yield is increased by 21.6% and reaches 1.63g/L. Thus, the requirement of industrial production is satisfied.

The invention relates to the field of starch sugar preparation, and discloses a compound amylase preparation, an application thereof in starch liquefaction and a starch liquefaction method. The compound amylase preparation comprises L-type amylase and S-type amylase, wherein the amino acid sequence of the L-type amylase has at least 95% sequence consistency with the amino acid sequence shown in SEQ ID NO: 1, and the amino acid sequence of the S-type amylase has at least 95% sequence consistency with the amino acid sequence shown in SEQ ID NO: 2. The method comprises the following steps of: adding the compound amylase preparation into starch feed liquid, and spraying. The compound amylase preparation can rapidly reduce the viscosity of a system in high-concentration starch feed liquid (under the condition of relatively low water activity), enables the feed liquid to be uniformly mixed, is favorable for pumping, has better thermal stability, can be sprayed and liquefied at a higher temperature, enables starch particles to be completely destroyed, and is favorable for complete liquefaction.

The invention provides a fungal amylase preparation method. The fungal amylase preparation method comprises the following steps that bacillus subtilis reacts with raw material starch to obtain alpha-amylase liquid; the alpha-amylase liquid is purified to obtain a purified alpha-amylase liquid; the purified alpha-amylase liquid is washed, precipitated and dried to obtain the alpha-fungal amylase. According to the fungal amylase preparation method, the acquisition rate of fungal amylase is improved, the production efficiency is further improved, and the production cost is saved.