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3 results about "Amylase preparation" patented technology

Preparation method of a halophilic archaea-derived amylase and application thereof

PendingCN122128279AHydrolasesFermentationLiquid mediumProkaryote organisms
This invention belongs to the field of genetic engineering and relates to a method for preparing amylase derived from halophilic archaea and its application; based on halophilic archaea... Haloarcula sp. ZJK10-12、 Haladaptatus The genes for α-amylase, maltose amylase, and glucoamylase were obtained from the whole genome sequence of sp. ZJK29-3. Recombinant plasmids were constructed using primer design and molecular cloning, and transformed into prokaryotic host bacteria to obtain the corresponding recombinant strains. The bacteria were enriched and cultured in Hv-YPC liquid medium, and the cells were collected and lysed by sonication. α-amylase, maltose amylase, and glucoamylase were obtained using nickel column affinity chromatography. The amylases exhibit excellent enzymatic properties and outstanding salt tolerance. Their application in the fermentation of high-salt, dilute soy sauce can improve raw material utilization and product quality, demonstrating good industrial applicability and promotional value.
Owner:JIANGSU UNIV

Freeze drying device for amylase preparation

The utility model belongs to the field of freeze-drying devices, and particularly relates to a freeze-drying device for amylase preparation, which comprises an outer main body, refrigeration equipment is fixedly connected to the bottom end in the outer main body, a box door is hinged to the left side of the rear end of the outer main body, and a cooling dustproof mechanism is arranged in the outer main body; the cooling dustproof mechanism comprises a fixing plate, the fixing plate is arranged at the right side end in the outer main body, and the fixing plate is fixedly connected with the outer main body; through the design of the cooling dustproof mechanism, the function of active cooling is achieved, and the problems that an existing device is not provided with an assembly capable of being used for rapid cooling, refrigeration equipment can generate heat during use, if the heat is not discharged in time for cooling, the device is prone to being damaged due to the influence of high temperature, and the service life of the device is prolonged are solved. The service life is shortened, and the service life of the device is prolonged.
Owner:NANJING NONGFENG BIOTECHNOLOGY CO LTD

Biomass high-transparency solid glue stick and preparation method thereof

PendingCN122037823ANon-macromolecular adhesive additivesStarch derivtive adhesivesTaurineTackifier
The invention belongs to the field of office stationery materials, and particularly relates to a biomass high-transparency solid glue stick which is prepared from the following raw materials in parts by weight: 10-30 parts of cassava starch, 0.1-0.8 part of an amylase preparation, 0.2-2 parts of a pH regulator, 2-4 parts of an oxidizing agent, 10-15 parts of a humectant, 1-5 parts of a high-transparency excipient, 1-6 parts of a tackifier, 0.1-0.5 part of a defoaming agent, 0.5-2 parts of other auxiliaries and 40-85 parts of water. The high-transparency excipient is a fatty acid substance and is selected from one or more of myristic acid, lauric acid, coconut oil, taurine and coco-sulfonic acid. According to the biomass high-transparency solid glue stick and the preparation method thereof provided by the invention, the problems that starch-based solid glue in the prior art is poor in transparency and insufficient in viscosity, and cannot give consideration to both high transparency and high adhesive property are solved.
Owner:LUOHE LOUFOR STATIONERY MFG