7 results about "Amylase preparation" patented technology

The invention belongs to the technical field of microorganisms, and particularly relates to a novel strain of brevibacterium and application thereof. The strain is separated from rapana venosa, is a new species of Brevibacterium, is named as Brevibacterium sp. M-17, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241860. The Brevibacterium sp. M-17 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, escherichia coli, shewanella, enterobacter aerogenes, staphylococcus aureus and proteus vulgaris, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate gelatinase, cellulase and amylase, and can be used for preparing an anti-inflammatory agent. The strain can be applied to preparation of bacteriostatic agents, antioxidants, alpha-glucosidase inhibitors, gelatinase, cellulase and amylase, can also be applied to industrial production instead of the bacteriostatic agents, the antioxidants, the gelatinase, the cellulase and the amylase in a viable bacterium form, and has good development and application prospects.

The invention provides a preparation method of medium-temperature amylase. The preparation method comprises the following steps: S1, selecting bacillus licheniformis to prepare a seed solution; s2, the seed solution is inoculated into a fermentation medium for fermentation culture, in the fermentation process, when the concentration of residual sugar is reduced to 1.0-1.5 g / L, soluble starch is fed according to a rate equation Rt = R0ekt, R0 is the initial feeding rate and is 2 g / L / h, k is the growth rate constant and is 0.4 h <-1 >, and t is the time after feeding and is h; and S3, separating the liquid fermented in the step S2 to obtain the medium-temperature amylase. According to the method, a dynamic index fed-batch technology is adopted, residual sugar is maintained in an ideal interval of 1.0-2.0 g / L, carbon repression is relieved, and the enzyme activity is improved to 18,500 U / mL.

This invention belongs to the field of genetic engineering and relates to a method for preparing amylase derived from halophilic archaea and its application; based on halophilic archaea... Haloarcula sp. ZJK10-12、 Haladaptatus The genes for α-amylase, maltose amylase, and glucoamylase were obtained from the whole genome sequence of sp. ZJK29-3. Recombinant plasmids were constructed using primer design and molecular cloning, and transformed into prokaryotic host bacteria to obtain the corresponding recombinant strains. The bacteria were enriched and cultured in Hv-YPC liquid medium, and the cells were collected and lysed by sonication. α-amylase, maltose amylase, and glucoamylase were obtained using nickel column affinity chromatography. The amylases exhibit excellent enzymatic properties and outstanding salt tolerance. Their application in the fermentation of high-salt, dilute soy sauce can improve raw material utilization and product quality, demonstrating good industrial applicability and promotional value.

The invention belongs to the technical field of microorganisms, and particularly relates to a new strain of microbacterium and application thereof. The bacterial strain is separated from rapana venosa, is a new species of Microbacterium sp. M-28, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has a preservation number of CCTCC NO: M 20241861. The invention also discloses a method for preparing the bacterial strain. The microbacterium sp. M-28 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, shewanella, staphylococcus aureus and proteusbacillus vulgaris, has obvious acetylcholin esterase inhibitory activity, has obvious antioxidant activity, has alpha-glucosidase inhibitory activity, can generate casein enzyme and amylase, and can be used for preparing a food additive. The strain can be applied to preparation of a bacteriostatic agent, an acetylcholin esterase inhibitor, an antioxidant, an alpha-glucosidase inhibitor, casein and amylase, can also replace the bacteriostatic agent, the antioxidant, the casein and the amylase in a viable form to be applied to industrial production, and has a very good development and application prospect.

The utility model belongs to the field of freeze-drying devices, and particularly relates to a freeze-drying device for amylase preparation, which comprises an outer main body, refrigeration equipment is fixedly connected to the bottom end in the outer main body, a box door is hinged to the left side of the rear end of the outer main body, and a cooling dustproof mechanism is arranged in the outer main body; the cooling dustproof mechanism comprises a fixing plate, the fixing plate is arranged at the right side end in the outer main body, and the fixing plate is fixedly connected with the outer main body; through the design of the cooling dustproof mechanism, the function of active cooling is achieved, and the problems that an existing device is not provided with an assembly capable of being used for rapid cooling, refrigeration equipment can generate heat during use, if the heat is not discharged in time for cooling, the device is prone to being damaged due to the influence of high temperature, and the service life of the device is prolonged are solved. The service life is shortened, and the service life of the device is prolonged.

The invention belongs to the technical field of microorganisms, and particularly relates to a new strain of psychrobacter and application thereof. The bacterial strain is separated from rapana venosa, is a new species of psychrobacter, is named as Psychrobacter sp. M-3, is registered and preserved in the China Center for Type Culture Collection (CCTCC), and has the preservation number of CCTCC NO: M 20241859. The Psychrobacter sp. M-3 strain disclosed by the invention has obvious bacteriostatic activity on bacillus subtilis, shewanella, escherichia coli, proteusbacillus vulgaris and staphylococcus aureus, has obvious antioxidant activity and alpha-glucosidase inhibitory activity, can generate pectinase and amylase, and can be used for preparing the Psychrobacter sp. M-3 strain. The strain can be applied to preparation of bacteriostatic agents, antioxidants, alpha-glucosidase inhibitors, pectinase and amylase, can also be applied to industrial production instead of the bacteriostatic agents, the antioxidants, casein and the amylase in a viable bacterium form, and has good development and application prospects.

The invention belongs to the field of office stationery materials, and particularly relates to a biomass high-transparency solid glue stick which is prepared from the following raw materials in parts by weight: 10-30 parts of cassava starch, 0.1-0.8 part of an amylase preparation, 0.2-2 parts of a pH regulator, 2-4 parts of an oxidizing agent, 10-15 parts of a humectant, 1-5 parts of a high-transparency excipient, 1-6 parts of a tackifier, 0.1-0.5 part of a defoaming agent, 0.5-2 parts of other auxiliaries and 40-85 parts of water. The high-transparency excipient is a fatty acid substance and is selected from one or more of myristic acid, lauric acid, coconut oil, taurine and coco-sulfonic acid. According to the biomass high-transparency solid glue stick and the preparation method thereof provided by the invention, the problems that starch-based solid glue in the prior art is poor in transparency and insufficient in viscosity, and cannot give consideration to both high transparency and high adhesive property are solved.