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Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase

A technology of high-temperature amylase and bacteria, applied in the field of bioengineering, can solve problems that have not been reported and achieve good acid-resistant activity

Inactive Publication Date: 2009-08-26
无锡德冠生物科技有限公司
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  • Abstract
  • Description
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AI Technical Summary

Problems solved by technology

[0029] However, effective strains and enzyme preparations of acid-resistant pH 3.8-6.5 have not been reported yet.

Method used

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  • Acid-proof high-temperature amylase strain and method for producing acid-proof high-temperature amylase

Examples

Experimental program
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Effect test

Embodiment 1

[0047] Slope formula (calculated in g / L): beef juice 4, peptone 10, yeast powder 3, NaCl3, starch 10, glucose 50, agar 15-25; culture temperature 35°C, culture time: 30h;

[0048] Shake bottle formula (calculated in g / L): bean cake powder 40, cottonseed cake powder 30, (NH 4 ) 2 SO 4 5. CaCl 2 0.5, corn steep liquor 30, lactose 150, sodium citrate 5, dipotassium hydrogen phosphate 15, potassium dihydrogen phosphate 7, culture temperature 40°C, culture time: 30h;

[0049] Seed tank formula (calculated in g / L): glucose 75, yeast powder 30, cottonseed cake powder 15, ammonium sulfate 5, sodium citrate 8, CaCl 2 0.5, P.P.E. (polyether defoamer) 0.5, dipotassium hydrogen phosphate 7, potassium dihydrogen phosphate 7, fermentation temperature: 35°C, ventilation: 0.8V.V.M, tank pressure: 0.8kg / cm 2 , Time: 36h, seed tank volume: 500L, 60% charge.

[0050] Production tank formula (calculated in g / L): glucose 75, yeast powder 30, cottonseed cake powder 15, ammonium sulfate 4, sodi...

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Abstract

The invention provides an acid-proof high-temperature amylase strain and a method for producing acid-proof high-temperature amylase, and belongs to the technical field of biological engineering. The invention discloses Bacillus licheniformis BL-mut 08 for producing the acid-proof high-temperature amylase, which has the preservation number of CCTCC NO: M207193, and is obtained through compound mutation of the prior Bacillus licheniformis system, preservation, rejuvenation and a selective breeding method; and then the Bacillus licheniformis is optimized by a culture medium formulation and optimized by fermenting technological conditions so as to establish the method for producing the acid-proof high-temperature amylase, wherein the method comprises the following steps: air culturing the Bacillus licheniformis for about 72 hours at a temperature of 37 DEG C to reach an enzyme generating peak; and obtaining concentrated enzyme fluid through flocculating treatment, pressure filtration and ultra-filtration concentration or obtaining different amylase preparations such as micro pills, granules or powder through other post treatment modes. The produced high-temperature amylase has good acid-proof activity, and can be widely applied in the food and medicament industries, particularly the industries such as alcoholic fermentation, monosodium glutamate, antibiotics, textile and papermaking.

Description

field of invention [0001] An acid-resistant high-temperature amylase strain and a method for producing acid-resistant high-temperature amylase belong to the technical field of bioengineering. Background technique [0002] High-temperature amylase is one of the most successfully industrially applied enzyme preparations, because enzymatic hydrolysis can control starch hydrolysis, reaction specificity and product stability to a greater extent. Mild reaction conditions include low reaction temperature, near-neutral pH, etc., thereby reducing energy consumption in production, reducing production costs and environmental pollution, increasing production and bringing beneficial economic benefits, social benefits and other advantages . [0003] Amylase has been widely used in the desizing of the textile industry, the treatment of starch pulp in the paper industry, the food industry such as caramel, beer, rice wine, glucose, monosodium glutamate, antibiotics and other industries; in ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N9/28C12R1/10
Inventor 陶丁伟魏晨飞周伟峰王清声
Owner 无锡德冠生物科技有限公司
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