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Acid-resistant enzyme preparation particles and preparation method thereof

An enzyme preparation and acid-resistant technology, which is applied in the direction of pharmaceutical formulas, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problem of increased organic solvent pollution, hidden safety hazards of organic solvents, and high cost of coating materials, etc. problem, to achieve the effect of improving the enzyme activity retention rate, less batch-to-batch variation, and low equipment requirements

Pending Publication Date: 2022-07-22
TIANJIN BIOFEED TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are some problems in the use of coating technology in the feed industry: although the coating technology of certain varieties has been used in the feed additive industry for a long time, such as coating vitamins, the application of this technology is not wide enough, especially for slow control. The release technology is still in its infancy and requires in-depth and systematic research; the cost of coating film materials is high, which leads to high prices of coated products, and further research is needed on the application of coating technology in the field of feed additives, especially enzyme preparations
Among them, ethyl cellulose needs to be dissolved in organic solvents, which increases the pollution of organic solvents
[0007] Disclosed in the patent document of CN105265756B is a kind of preparation method of coating type compound enzyme, the coating process of this method adopts post-spraying, wherein the coating material needs to be dissolved with ethanol, the equipment requires explosion-proof, and the use of a large amount of organic solvents has safety hazards. Hidden dangers and environmental pollution

Method used

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  • Acid-resistant enzyme preparation particles and preparation method thereof
  • Acid-resistant enzyme preparation particles and preparation method thereof
  • Acid-resistant enzyme preparation particles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Embodiment 1, a kind of preparation method of acid-resistant phytase

[0026] (1) An acid-resistant phytase granule is provided in this embodiment, and its raw materials include solid raw materials and enteric emulsion, wherein the mass percentage of each component of the solid raw materials: phytase 13%, starch 50%, maltodextrin 17%, talc 20%. Enteric emulsion (30% solid content of methacrylic acid-ethyl acrylate copolymer aqueous solution, the following enteric emulsion refers to this solution) is added at 15% of the solid raw material weight.

[0027] The specific preparation method is as follows:

[0028] Step 1: According to the formula ratio, mix the phytase powder preparation with the rest of the solid raw materials uniformly, first dry-mix for 3 minutes, then add enteric emulsion for wet mixing, and mix for 5 minutes to prepare soft material.

[0029] Step 2: The prepared soft material is shaken or extruded into 20-60 mesh pellets.

[0030] Step 3: Dry the pe...

Embodiment 2

[0047] Embodiment 2, a kind of preparation method of acid-resistant glucose oxidase

[0048] An acid-resistant glucose oxidase granule, the raw materials of the granule include solid raw material and enteric emulsion, wherein the mass percentage of each component of the solid raw material: glucose oxidase 15%, starch 50%, maltodextrin 10%, talc 25% %. The enteric emulsion was added at 15% by weight of the solid raw material.

[0049] According to the process of Example 1, acid-resistant glucose oxidase granules, powder-type glucose oxidase granules, and ordinary glucose oxidase granules were prepared respectively for acid resistance evaluation, and the enzyme activity retention rate was calculated respectively. The specific results are shown in Table 2.

[0050] Table 2 Effects of glucose oxidase granules prepared by different methods on acid resistance

[0051]

[0052] It can be seen from the results in Table 2 that the enzyme activity retention rate of acid-resistant ...

Embodiment 3

[0053] Embodiment 3, the influence of the addition amount of enteric emulsion on acid resistance

[0054] According to the preparation method of acid-resistant glucose oxidase enzyme granules in Example 1, the addition amount of the enteric emulsion was screened, and the enzyme activity retention rate was used as the evaluation index. The results are shown in Table 3.

[0055] Table 3 Effects of different enteric emulsion additions on acid resistance and enzyme activity retention rate of glucose oxidase granules

[0056]

[0057]

[0058] It can be seen from the results in Table 3 that the addition amount of enteric emulsion is negatively correlated with the original enzyme activity of glucose oxidase granules, and the addition amount of enteric emulsion is positively correlated with the enzyme activity retention rate. Considering the application effect and cost, the recommended addition amount is 5-30%, more preferably 5-20%, and the preferred addition amount is 15%. ...

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Abstract

The invention discloses an acid-resistant enzyme preparation particle, the raw materials of the particle comprise a solid raw material composed of a powdery enzyme preparation and an auxiliary material, and an enteric emulsion, the powdery enzyme preparation and the auxiliary material are uniformly mixed, then the enteric emulsion is added, granulation and drying are performed after uniform mixing, and the acid-resistant enzyme preparation particle can be obtained. The enzyme preparation particles are good in acid resistance, compared with a traditional coating method, acid-resistant tests prove that the enzyme activity retention rate is increased by 23%, and application of the enzyme preparation in animal bodies is better facilitated. Compared with a traditional coating mode, the preparation method disclosed by the invention is simple to operate, low in equipment requirement and free from the use risk of an organic solvent, the production cost is greatly saved, and the energy consumption is reduced.

Description

technical field [0001] The present application relates to the field of enzyme preparation processing, in particular, to an acid-resistant enzyme preparation particle and a preparation method thereof. Background technique [0002] The active ingredients of feed additives, especially enzyme preparations, will decrease significantly under acidic conditions. Therefore, the quality of acid resistance will seriously affect its effect in the gastrointestinal tract of animals. At present, there are two main methods to improve the acid resistance of enzyme preparations: 1) genetic modification at the molecular level to improve its acid resistance; 2) post-treatment process development, which mainly uses coating technology to protect its active ingredients and make them It is protected from the damage of gastric acid and endogenous enzymes to achieve the purpose of fixed-point release, so as to facilitate its full effect. [0003] Coating technology is a technology used in feed ingr...

Claims

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Application Information

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IPC IPC(8): A61K9/16A61K38/44A61K38/46A61K38/47A61K38/48A61K47/36A61K47/02
CPCA61K38/48A61K38/465A61K38/443C12Y101/03004A61K38/47C12Y302/01008A61K9/1652A61K9/1611A61K9/1682
Inventor 刘明娜陈凯王海燕曹春红白燕丽刘敏邓启生张广民蔡辉益
Owner TIANJIN BIOFEED TECH CO LTD
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