Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of liquefied amylases capable of resisting superhigh temperature

A liquefying amylase, ultra-high temperature technology, applied in biochemical equipment and methods, microorganism-based methods, enzymes, etc., can solve the problems of high production cost, difficult temperature tolerance of amylase to exceed 100 ° C, containing and other problems

Inactive Publication Date: 2014-03-05
HUNAN HUXIANG BIOTECH CO LTD
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing methods for producing high-temperature-resistant liquefaction amylase mainly have the following disadvantages: first, the production cost is relatively high; Most enterprises use ammonium sulfate salting out or alcohol precipitation to obtain solid amylase after filtration and drying. This product contains residues, ammonium sulfate and other impurities, and its application range is limited.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

specific Embodiment approach

[0016] The present invention will be further described below in conjunction with examples, but these examples should not be construed as limiting the protection scope of the present invention. Without departing from the spirit and essence of the present invention, any modifications or replacements made to the method of the present invention belong to the protection scope of the present invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and each raw material added is a commercially available product unless otherwise specified.

[0017] Step 1, agar medium inoculation culture: (1) Agar medium preparation, medium formula: tryptone 1.2g, yeast extract 0.6g, NaCl 1.0g, agar 2.0g, soluble starch 0.28g, pH6.8, the rest It is water, with a total weight of 100g; after mixing the 100g ingredients, put them in a petri dish, sterilize at 0.1MPa for 33min, cool to room temperature, and make an agar ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for producing coenzymes Q10 via the fermentation of genetic recombinant bacteria. According to the method, NAD (Nicotinamide Adenine Dinucleotide) kinase genes are transferred into strains of reducing coenzymes Q10 with high-efficiency expression. The culture condition of optimizing the recombinant bacteria comprises (1) cant activation; (2) seed culture; and (3) fermentation culture. The recombinant bacteria constructed by a genetic engineering means are strong in stability; compared with original strains, the coenzymes Q10 of the recombinant bacteria has the advantages that the yield is increased by 21.6% and reaches 1.63g / L. Thus, the requirement of industrial production is satisfied.

Description

technical field [0001] The invention relates to a method for preparing amylase, in particular to a method for preparing amylase which still has relatively high activity at high temperature and is used for starch liquefaction. Background technique [0002] Starch is widely used in food, medicine, chemical industry, papermaking, petroleum and other fields, and its development prospects are very broad. With the continuous development of science and technology, the increasing number of new products such as starch, starch sugar and starch derivatives and the development of starch deep-processing products have accelerated and promoted the development of the starch industry. [0003] my country's starch and starch products industry is developing rapidly. The total starch output is increasing at an average annual rate of about 10-15%. It has become one of the largest starch producers in the world, laying a considerable material foundation for the development and production of starch...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/28C12R1/10
CPCC12N9/2411
Inventor 林亲录袁四岳孙术国吴跃梁盈付湘晋肖华西
Owner HUNAN HUXIANG BIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com