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Fungal amylase preparation and storage method

A technology of fungal amylase and amylase, applied in biochemical equipment and methods, methods based on microorganisms, enzymes, etc., can solve the problems of low acquisition rate, achieve the effects of improving production efficiency, increasing acquisition rate, and saving production costs

Inactive Publication Date: 2022-01-21
湖南康捷生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, in the preparation process of fungal amylase, the acquisition rate of enzyme liquid to powdered enzyme preparation is low, so there is a lack of a method to improve the acquisition rate of fungal amylase

Method used

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  • Fungal amylase preparation and storage method
  • Fungal amylase preparation and storage method
  • Fungal amylase preparation and storage method

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0039] according to Figure 1-2 Shown, the embodiment of the present invention provides that the present invention provides a kind of fungal amylase preparation method, comprising:

[0040] Use Bacillus subtilis to react with the raw material starch to obtain α-amylase liquid; further, inoculate the strain on the culture medium, and cultivate it for 60-80 hours at a culture temperature of 25-40 degrees Celsius; then culture it on the culture medium The strains in the seed tank are transferred to the seed tank for cultivation; cultivation time: 10-18 hours, cultivation temperature: 25-40 degrees Celsius; Fermentation is terminated after secondary cultivation until the enzyme activity does not increase; the fermented product in the fermenter is destroyed by protease, and cooled and filtered to obtain a filtrate.

[0041] purifying the α-amylase to obtain a purified α-amylase solution; washing the filtrate with water, concentrating in vacuo, adding a salt out product, adding dia...

experiment example 1

[0049] Utilize Bacillus subtilis to react with the raw material starch to obtain α-amylase liquid; further, inoculate the strain on the culture medium, and cultivate it for 65 hours, and the culture temperature is 40 degrees Celsius; then transfer the strain cultured on the culture medium to Inoculate into the seed tank for cultivation; cultivation time: 10 hours, cultivation temperature: 40 degrees Celsius; after the cultivation of the strain in the seed tank is completed, transfer the cultured strain into the fermenter for stirring and secondary cultivation until the enzyme activity does not increase Finally, the fermentation is terminated; the fermented product in the fermenter is destroyed by protease, and cooled and filtered to obtain a filtrate.

[0050] Purifying the α-amylase liquid to obtain a purified α-amylase liquid; washing the filtrate with water, concentrating in vacuo, adding a salt out product, adding diatomaceous earth to the salt out product, and performing p...

experiment example 2

[0058] Use Bacillus subtilis to react with raw material starch to obtain α-amylase liquid; further, inoculate strains on the culture medium, and cultivate it for 76 hours, and the culture temperature is 36 degrees Celsius; transfer the strains cultured on the culture medium to Inoculate into the seed tank for cultivation; cultivation time: 12 hours, cultivation temperature: 36 degrees Celsius; after the cultivation of the strain in the seed tank is completed, transfer the cultured strain into the fermenter for stirring and secondary cultivation until the enzyme activity does not increase Finally, the fermentation is terminated; the fermented product in the fermenter is destroyed by protease, and cooled and filtered to obtain a filtrate.

[0059] Purifying the α-amylase liquid to obtain a purified α-amylase liquid; washing the filtrate with water, concentrating in vacuo, adding a salt out product, adding diatomaceous earth to the salt out product, and performing pressure filtrat...

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Abstract

The invention provides a fungal amylase preparation method. The fungal amylase preparation method comprises the following steps that bacillus subtilis reacts with raw material starch to obtain alpha-amylase liquid; the alpha-amylase liquid is purified to obtain a purified alpha-amylase liquid; the purified alpha-amylase liquid is washed, precipitated and dried to obtain the alpha-fungal amylase. According to the fungal amylase preparation method, the acquisition rate of fungal amylase is improved, the production efficiency is further improved, and the production cost is saved.

Description

technical field [0001] The invention relates to the technical field of fungal amylase, in particular to a method for preparing and storing fungal amylase. Background technique [0002] Fungal amylase, also known as fungal α-amylase (1,4-α-D-glucan hydrolase), is refined from Aspergillus oryzae through submerged culture, extraction and other processes. The enzyme is an endo-amylase, which can rapidly hydrolyze the α-1,4 glucosidic bond in gelatinized starch, amylose and amylopectin aqueous solution to produce soluble dextrin and a small amount of maltose and glucose. Prolonged reaction will produce a syrup with a large amount of maltose and a small amount of glucose. [0003] At present, in the preparation process of fungal amylase, the acquisition rate of enzyme liquid to powdered enzyme preparation is low, so there is a lack of a method to improve the acquisition rate of fungal amylase. Contents of the invention [0004] The invention provides a preparation and storage ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/28C12N1/20B65D88/74B65D90/00B65D90/36B65D90/48C12R1/125
CPCC12N9/2417C12N1/20B65D88/744B65D90/00B65D90/36B65D90/48C12Y302/01001Y02W90/10
Inventor 袁群陈耿鸿陈武根黄石桥李书文石团结游启进何满春雷敏严钦菊
Owner 湖南康捷生物科技有限公司
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