42 results about "Blastomycetes" patented technology

The present invention relates to a composition comprising at least one biological control agent selected from the group consisting of Bacillus chitinosporus AQ746 (NRRL Accession No, B-21618), Bacillus mycoides AQ726 (NRRL Accession No, B-21664), Bacillus pumilus (NRRL Accession No, B-30087), Bacillus pumilus AQ717 (NRRL Accession No, B-21662), Bacillus sp, AQ175 (ATCC Accession No. 55608), Bacillus sp, AQ177 (ATCC Accession No, 55609), Bacillus sp. AQ178 (ATCC Accession No, 53522), Bacillus subtilis AQ743 (NRRL Accession No, B-21665), Bacillus subtilis AQ713 (NRRL Accession No, B- 21661), Bacillus subtilis AQ153 (ATCC Accession No, 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. B-21530), Bacillus thuringiensis AQ52 (NRRL Accession No, B- 21619), Muscodor albus 620 (NRRL Accession No, 30547), Muscodor roseus A3-5 (NRRL Accession No. 30548), Rhodococcus globerulus AQ719 (NRRL Accession No. B-21663), Streptomyces galbus (NRRL Accession No. 30232), Streptomyces sp. (NRRL Accession No. B-30145), Bacillus thuringiensis subspec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL Accession No. B-50421), and Bacillus subtilis AQ 30004 (NRRL Accession No. B-50455) and / or a mutant of these strains having all the identifying characteristics of the respective strain, and / or a metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens and at least one fungicide (I) in a synergistically effective amount, Furthermore, the present invention relates to the use of this composition as well as a method for reducing overall damage of plants and plant parts.

The invention discloses a cellulose degradation bacterium CX9 from termite gut and application thereof. The strain is preserved in the China Center For Type Culture Collection in No.3, No.1 yard on Beichen West Road, Chaoyang District, Beijing on August 27th, 2018 and has the preservation number of CGMCC No.16343. The strain is gram-positive bacilli without capsules, endogenous spores can be seenafter spores are dyed, bacterial colonies are each in a crack shape and are independent of one other or connected to form a piece, and the bacterial colonies have the white color and complete edges. The strain is a facultative anaerobic strain capable of generating cellulase; the strain has the CMCase enzyme activity of 70.4 IU / mL and CX enzyme activity of 25.23 IU / mL within 48 hours. The strain has quite high biodiversity value and also has further research scientific value in the development and application of the fermentation and reutilization technology of cellulose-enriched crops and traditional Chinese medicines.

The invention discloses a screening method and application of a strain for producing 1,3-propanediol, relating to 1,3-propanediol. A screening method and application of clostridium butyricum Gen160 and the strain screening method for producing 1,3-propanediol with waste glycerol are provided. The screening method comprises the steps as follows: adding collected samples to cabbage juice, putting the samples into a water bath after an enrichment culture to kill nonsporeforms, transferring the samples to a clostridium multiplication liquid medium, carrying out an anaerobic culture and then putting the samples into the water bath again, transferring the samples to a fermentation medium, carrying out a high performance liquid chromatograph to analyze fermentation products after the anaerobic selective enrichment culture and selecting positive strains capable of producing 1,3-propanediol; separating out single colonies by a high layer agar column method; carrying out an aerobiotic culture and the anaerobic culture to remove facultative anaerobes and to obtain strict anaerobes, analyzing the fermentation products and selecting the strains to carry out 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis and identification, wherein the strains are capable of producing 1,3-propanediol and the cultural characteristics and the colonial morphologies confirm to the cultural characteristics of the clostridium butyricum consistently.

The present invention provides a meat extract and a production method thereof. The meat extract contains no detectable microorganisms other than spore-forming bacteria and includes 60 mmol / l or more of phosphate ion, wherein the spore-forming bacteria are microorganisms selected from bacillus, sporolactobacillus, clostridium, and sporosarcina. The meat extract is obtained from a material containing muscle tissues or bone tissues of livestock. The production method includes: obtaining the liquid extract from a material containing muscle tissues or bone tissues of livestock; and adjusting the phosphate ion concentration of the liquid extract to 60 mmol / I or more to inhibiting proliferation of the spore-forming bacterium in the meat extract.

The invention discloses a microbial environment protectant, which is characterized by comprising mixed bacteria and a traditional Chinese medicine extract, wherein the mixed bacteria are formed by Rhodopseudomonas palustris, Bacillus Subtilis and Lactobacillus delbruckii. The weight ratio of the Rhodopseudomonas palustris to the Bacillus Subtilis to the Lactobacillus delbruckii is 3:4:7; the volume ratio of the traditional Chinese medicine extract to the mixed bacteria is 1-2 percent. The microbial environment protectant disclosed by the invention has the positive effects that through performing mixed fermentation on the compound bacterial liquid and the traditional Chinese medicine extract, the traditional Chinese medicine ingredients are further activated, and the traditional Chinese medicine ingredients and thallus exoenzyme interact to restrain the incubation of ova, and the generation of adult flies is reduced. As a traditional Chinese medicine and bacterial liquid fermentation medium is special in smell, a trapping and killing effect is performed on the adult flies, and the bad smell of a smelly source can also be effectively prevented from being emitted. The microbial environment protectant disclosed by the invention is harmless to people, is low in cost, simple in preparation process, simple and convenient in operation and remarkable in deodorization and fly killing effect. The problems that mosquitoes and the flies and foul smell exist in refuse landfills, waste transfer stations and digestion tanks are well solved, and a good effect is achieved.

The invention discloses a method used for preparing a Paenibacillus polymyxa biological pesticide wettable powder based on liquid fermentation and a formula dosage form. A Paenibacillus polymyxa brothadopted in the method is capable of solving a problem that the spore conversion rate of conventional liquid fermentation is low, and the spore conversion rate of the method is higher than 98%. The obtained Paenibacillus polymyxa broth is composed of active Paenibacillus polymyxa; the stress resistance is excellent; the service life is long; long time storage can be realized; self life is long; the method is suitable for fermentation technology, and industrialized production. The invention also discloses a production method of a Paenibacillus polymyxa concentrate and the Paenibacillus polymyxabiological pesticide wettable powder; the preparation method is simple; spore recovery rate is 98% or higher; a plurality of spore protection technologies are adopted; product self life is prolongedto be 18months or longer; and commercial value is achieved.

The present invention discloses a cellulose-degrading bacterium CX9 from termite gut. The strain was preserved in General Microbiology Center of China Microbiological Culture Preservation Management Committee, No. 1, No. 1 Beichen West Road, Chaoyang District, Beijing on August 27, 2018. , the deposit number is: CGMCC No.16343. The strain is Gram-positive bacillus, without capsule, endophytic spores can be seen in spore staining, the colony is cracked, the colony is single or connected sheet, the color of the colony is white, and the edge of the colony is complete. The strain is a facultative anaerobic strain producing cellulase; within 48 hours, the CMCase activity of the strain was 70.4IU / mL, and the CX activity was 25.23IU / mL. The strain not only has high biodiversity value, but also has scientific value for further research in the development and application of cellulose-enriched crops and traditional Chinese medicine fermentation and reuse technology.

The invention relates to the technical field of microorganisms, in particular to a bacillus velei and its application. The preservation number of Bacillus Velez Lpl-wx in the present invention is CGMCC No.17045. The bacterial strain can be applied to the preparation of astaxanthin esterase, and because the Bacillus velaisi belongs to prokaryote, the fermentation process is fast and the enzyme production efficiency is high, and the astaxanthin esterase can be obtained quickly and efficiently. The strain can be further applied to the preparation of astaxanthin monomers, which can effectively degrade astaxanthin esters and obtain astaxanthin monomers, providing a green and efficient catalytic pathway with less by-products, which is a good catalyst for the production of astaxanthin monomers. Preparation and promotion provide the technical basis.

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a compound bacillus inoculant. These include Bacillus methylotrophicus KNK‑1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. All the above strains were isolated and preserved by our laboratory. The most notable feature of the present invention is the application of the compound spore inoculant in promoting plant growth and / or preventing and controlling related plant diseases caused by phytopathogenic bacteria infection. , In addition, the average control effect on common plant diseases can reach 70%, which has great application value to the cultivation of economic plants.

The present invention provides a strain of anaerobic bacillus eupyroflavitas and application thereof, and the strain is anaerobic bacterium eupyroflavitas ( Anoxybacillus flavithermus ) Fzu200, which was registered and preserved in the General Microbiology Center of the China Microbiological Culture Collection Management Committee on December 24, 2018. The preservation number is: CGMCC No.17024. Recovery of heavy metal nickel in high temperature factory wastewater and high temperature mine wastewater. Compared with the traditional heavy metal treatment method, the method has less secondary pollution, low cost and high recovery rate.

The invention discloses microbial fermentation feed as well as a production method and an application thereof. The production method of the microbial fermentation feed comprises the following steps: (1) lactic acid bacteria, Clostridium butyricum Cb-2, yeast, Bacillus subtilis, a fermentation base-material and water are uniformly mixed, and a fermentation raw material is obtained; 2) aerobic fermentation and anaerobic fermentation are carried out in order for the fermentation raw material obtained in the step (1), and after fermentation, the feed is obtained. The microbial fermentation feed contains microecological viable organisms, and the total amount of beneficial bacteria is more than 5 x 10<9> cfu / g; the amount of mould and the content of mould toxin are lower than national limit standards; the feed is rich in organic acid, the content of lactic acid reaches 2.5%, and pH value is lower than 4.5; the titer of antibacterial peptide is higher than 1000 u / g, the titer of neutral protease is 150 u / g, the titer of alpha-amylase 300 u / g, dry weight for above, and the titer of total protease is higher than 600 u / g; the whole fermentation process is controlled in the range of 8-24 hours, the fermentation time is saved, and the dissipating energy is controlled below 3%.

The present invention discloses a cellulose-degrading bacterium CX10 from the gut of termites. The strain was preserved on August 27, 2018 in the Ordinary Microbiology Center of China Microbiological Culture Collection Management Committee, No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing. , the deposit number is: CGMCC No.16344. The strain is Gram-positive bacillus, without capsule, endophytic spores can be seen in spore staining, the colony is cracked and single, the color of the colony is white, and the edge of the colony is complete. The strain is a facultative anaerobic strain producing cellulase; within 48 hours, the FPA enzyme activity of the strain was 49.5U / mL, the CMCase activity was 95.0IU / mL, and the CX enzyme activity was 23.26IU / mL. The strain not only has high biodiversity value, but also has scientific value for further research in the development and application of cellulose-enriched crops and traditional Chinese medicine fermentation and reuse technology.