47 results about "Blastomycetes" patented technology

Disclosed is a preparation method of a compound bacteria coated microcapsule. Sodium alginate, CaCl2, polyethylene glycol and palmitate are used as a coating wall material mixed solution, which is stirred uniformly with compound bacteria at the temperature of 50-60 DEG C by a constant temperature magnetic stirrer; and the mixture is cooled and subjected to centrifugation to prepare the microcapsule. The compound bacteria is bacillus bacteria, lactic acid bacteria and yeast. The invention provides a simple preparation method, the prepared compound bacteria microcapsule has good encapsulation yield and encapsulation efficiency, and can pass through the stomach and dissolve in intestinal tracts, so as to avoid bacteria number loss of living bacteria in the stomach and produce the biggest effect in the intestinal tracts.

The invention discloses a screening method and application of a strain for producing 1,3-propanediol, relating to 1,3-propanediol. A screening method and application of clostridium butyricum Gen160 and the strain screening method for producing 1,3-propanediol with waste glycerol are provided. The screening method comprises the steps as follows: adding collected samples to cabbage juice, putting the samples into a water bath after an enrichment culture to kill nonsporeforms, transferring the samples to a clostridium multiplication liquid medium, carrying out an anaerobic culture and then putting the samples into the water bath again, transferring the samples to a fermentation medium, carrying out a high performance liquid chromatograph to analyze fermentation products after the anaerobic selective enrichment culture and selecting positive strains capable of producing 1,3-propanediol; separating out single colonies by a high layer agar column method; carrying out an aerobiotic culture and the anaerobic culture to remove facultative anaerobes and to obtain strict anaerobes, analyzing the fermentation products and selecting the strains to carry out 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis and identification, wherein the strains are capable of producing 1,3-propanediol and the cultural characteristics and the colonial morphologies confirm to the cultural characteristics of the clostridium butyricum consistently.

The invention discloses a bacillus licheniformis CPL618 as well as screening and application thereof. The strain CPL618 is identified as bacillus licheniformis, the strain CPL618 is preserved in the China Center for Type Microbiological Culture Collection (CCTCC), the preservation time is September 13, 2021, and the preservation number is CCTCC NO: M20211158. The bacillus licheniformis CPL618 disclosed by the invention has broad-spectrum antibacterial activity, and can antagonize the growth of gram-positive bacteria, gram-negative cocci and fungi. The invention provides an optimized industrial culture medium which can improve the yield of bacitracin A at lower cost, and the maximum yield can reach 2.3 g / L. The bacillus licheniformis disclosed by the invention has a wide utilization prospect in the aspects of preparation of antibacterial agents and production of bacitracin A.

The invention relates to a detection kit for sensitively and quickly detecting three spore production bacteria in food and a detection method thereof. The kit comprises a 5 U / mu L Taq DNA polymerase and PCR reaction solution, wherein the PCR reaction solution contains 10 mM of Tris.HCl, 50 mM of KCl, 25 mM of MgCl2, 2.5 mM of dNTP and 10 mu M of each primer of the three spore production bacteria. By using the detection kit and the detection method, the clostridium botulinum, the clostridium perfringens and the bacillus cereus in the food can be detected with high sensitivity; besides, the detection takes a short time and has simple operations, thus the detection kit and the detection method can save a large number of labor and financial capacities and are suitable for the requirement on quick detection.

The invention provides a spore protective agent and application thereof, and a detection method of sporeformer producing content in a microbial fertilizer. The spore protective agent comprises normal saline with the sodium chloride concentration of 8-10% and polyaspartic acid which accounts for 1-5 wt.% of the normal saline. The sporeformer protective agent can protect the germinated spores, so that the spores can continue growth in the culture medium within the sterilization temperature of 70 DEG C, thereby ensuring the detection accuracy of the sporeformer content in the microbial fertilizer in the subsequent process.

The invention discloses a broad-spectrum applicable organic material decomposition agent. Strains mainly generating different lytic enzymes cooperate with each other, and two or more bacteria break antagonism and adapt to each other to reduce the adaptation time in the earlier stage of composting; spore bacteria is promoted to generate many pores in a proper environment, and the organic material is quickly heated and decomposed; and the obtained strain reaches certain content and has an quantitative index enabling quantitative use when in use. The organic decomposition agent disclosed by the invention has broad-spectrum adaptability and high efficiency.

The present invention relates to a composition comprising at least one biological control agent selected from the group consisting of Bacillus chitinosporus AQ746 (NRRL Accession No, B-21618), Bacillus mycoides AQ726 (NRRL Accession No, B-21664), Bacillus pumilus (NRRL Accession No, B-30087), Bacillus pumilus AQ717 (NRRL Accession No, B-21662), Bacillus sp, AQ175 (ATCC Accession No. 55608), Bacillus sp, AQ177 (ATCC Accession No, 55609), Bacillus sp. AQ178 (ATCC Accession No, 53522), Bacillus subtilis AQ743 (NRRL Accession No, B-21665), Bacillus subtilis AQ713 (NRRL Accession No, B- 21661), Bacillus subtilis AQ153 (ATCC Accession No, 55614), Bacillus thuringiensis BD#32 (NRRL Accession No. B-21530), Bacillus thuringiensis AQ52 (NRRL Accession No, B- 21619), Muscodor albus 620 (NRRL Accession No, 30547), Muscodor roseus A3-5 (NRRL Accession No. 30548), Rhodococcus globerulus AQ719 (NRRL Accession No. B-21663), Streptomyces galbus (NRRL Accession No. 30232), Streptomyces sp. (NRRL Accession No. B-30145), Bacillus thuringiensis subspec. kurstaki BMP 123, Bacillus subtilis AQ30002 (NRRL Accession No. B-50421), and Bacillus subtilis AQ 30004 (NRRL Accession No. B-50455) and / or a mutant of these strains having all the identifying characteristics of the respective strain, and / or a metabolite produced by the respective strain that exhibits activity against insects, mites, nematodes and / or phytopathogens and at least one fungicide (I) in a synergistically effective amount, Furthermore, the present invention relates to the use of this composition as well as a method for reducing overall damage of plants and plant parts.

The invention discloses a cellulose decomposing strain CX10 from a termite gut and application thereof. The strain is preserved in the General Microbiological Center of China Committee for Culture Collection of Microorganisms at the No.3, first yard, Beichen west road, Chaoyang district, Beijing, and the preservation number is CGMCC No.16344. The strain is a gram-positive bacillus and free of capsules, endogenous spores can be seen after spore staining, the bacterial colony is a single one and in a cracked shape, the bacterial colony is white, and the edge of the bacterial colony is complete.The strain is a facultative anaerobic strain for producing cellulase; within 48 hours, the FPA enzyme activity of the strain is 49.5 U / mL, the CMCase enzyme activity is 95.0 IU / mL, and the CX enzyme activity is 23.26 IU / mL. The strain is very high in biodiversity value, and has a scientific value of further research on cellulose-enriched crops and development and application of a traditional Chinese medicine fermentation recycling.

The invention discloses a Sclerotinia sclerotiorum-antagonizing nitrogen-fixing spore bacterium and its application. The nitrogen-fixing spore bacterium is Bacillus sp. GDSD212, which is preserved in General microbiological center of China Committee for Culture Collection of Microorganisms with a preservation number of CGMCC No.5035. The Bacillus sp. GDSD212 CGMCC No.5035 provided in the invention has high nitrogen-fixing activity, can effectively antagonize Sclerotinia sclerotiorum, and has a broad application prospect in production of nitrogen-fixing microorganism bacterial agents and bio-organic fertilizers.

The invention is applied to the technical field of microbial fermentation and provides a preparation method of bacillus amyloliquefaciens G5 spore bacteria powder. The method comprises the following steps: step one, preparing a spore-producing culture medium from corn starch, glucose, soybean meal, calcium carbonate, ammonium chloride, dipotassium phosphate, magnesium sulfate and manganese sulfate; step two, culturing a spore fermentation solution; and step four, preparing spore bacteria powder by adding ammonium sulfate and kieselguhr into the spore fermentation solution and then carrying outplate-frame filtration to obtain the bacillus amyloliquefaciens G5 spore bacteria powder. In the preparation method of the bacillus amyloliquefaciens G5 spore bacteria powder, the raw materials of the spore-producing culture medium are common, convenient and easily available, so that the production cost is reduced; a large number of spores exist in the obtained spore fermentation solution; the spore yield is greater than or equal to 98%; ammonium sulfate and kieselguhr are taken as flocculants, so that the preparation time of the spore bacteria powder is reduced; a large number of effective living bacteria exist in the obtained spore bacteria powder; the yield of the effective living bacteria is greater than or equal to 95%.

The invention discloses a cellulose degradation bacterium CX9 from termite gut and application thereof. The strain is preserved in the China Center For Type Culture Collection in No.3, No.1 yard on Beichen West Road, Chaoyang District, Beijing on August 27th, 2018 and has the preservation number of CGMCC No.16343. The strain is gram-positive bacilli without capsules, endogenous spores can be seenafter spores are dyed, bacterial colonies are each in a crack shape and are independent of one other or connected to form a piece, and the bacterial colonies have the white color and complete edges. The strain is a facultative anaerobic strain capable of generating cellulase; the strain has the CMCase enzyme activity of 70.4 IU / mL and CX enzyme activity of 25.23 IU / mL within 48 hours. The strain has quite high biodiversity value and also has further research scientific value in the development and application of the fermentation and reutilization technology of cellulose-enriched crops and traditional Chinese medicines.

The invention relates to the technical field of microorganisms, in particular to a preparation method and application of a complex bacillus agent. The complex bacillus agent comprises Bacillus methylotrophicus KNK-1, Bacillus cereus BCJB01 and Bacillus megaterium BMJBN02. The strains are separated and preserved in labs. The prominent characteristic of the complex bacillus agent is the applicationof the complex bacillus agent in promoting plant growth and / or controlling related plant diseases caused by plant pathogen infestation, has the growth promotion effect on plants of about 30% and has strong seedling index increased to about 92%. Besides, the complex bacillus agent has average control effect on common plant diseases up to 70% and has great application values for cultivation of economic plants.

The invention discloses a screening method and application of a strain for producing 1,3-propanediol, relating to 1,3-propanediol. A screening method and application of clostridium butyricum Gen160 and the strain screening method for producing 1,3-propanediol with waste glycerol are provided. The screening method comprises the steps as follows: adding collected samples to cabbage juice, putting the samples into a water bath after an enrichment culture to kill nonsporeforms, transferring the samples to a clostridium multiplication liquid medium, carrying out an anaerobic culture and then putting the samples into the water bath again, transferring the samples to a fermentation medium, carrying out a high performance liquid chromatograph to analyze fermentation products after the anaerobic selective enrichment culture and selecting positive strains capable of producing 1,3-propanediol; separating out single colonies by a high layer agar column method; carrying out an aerobiotic culture and the anaerobic culture to remove facultative anaerobes and to obtain strict anaerobes, analyzing the fermentation products and selecting the strains to carry out 16S rDNA (ribosomal deoxyribonucleic acid) sequence analysis and identification, wherein the strains are capable of producing 1,3-propanediol and the cultural characteristics and the colonial morphologies confirm to the cultural characteristics of the clostridium butyricum consistently.

An object of the present invention is to provide a novel culturing method by which spores can be efficiently produced. The present invention further provides a method for culturing sporulating bacteria, comprising adding a sporulation-inhibiting substance into a medium for culturing sporulating bacteria, wherein the carbon content in the medium is 9.1 g / L or more, and preferably further comprising a step of adding a sporulation-accelerating substance to the medium.

The invention discloses a composite microbial preparation with a biodegradable flocculant. The composite microbial preparation with the biodegradable flocculant comprises the following components in parts by mass: 30 parts of Bacillus coagulans with the strain number of CGMCC1.10302, 20 parts of Bacillussubtilis with the strain number of CGMCC1.15792, 10 parts of Cellulase with the concentration of 8000U / g and 140 parts of amylaceum. The composite microbial preparation with the biodegradable flocculant is successfully developed by screening high-performance Bacillus and an enzyme preparation and performing scientific compatibility aiming at the characteristics of the biodegradable flocculant, and can quickly raise the sludge fermentation temperature, kill harmful microorganisms in sludge and quickly decompose the sludge through fermentation under the normal sludge high-temperature fermentation production management condition.

The invention relates to the technical field of microorganisms, in particular to bacillus velezensis and an application thereof. The preservation number of the bacillus velezensis Lpl-wx is CGMCC No.17045. The bacterial strain can be applied to preparation of astaxanthin esterases. The bacillus velezensis belongs to prokaryotes, so that the fermentation process is quick, enzyme production efficiency is high, and the astaxanthin esterases can be quickly and efficiently obtained. The bacterial strain can be further applied to preparation of an astaxanthin single body and can effectively degradeastaxanthin esters, the astaxanthin single body can be obtained, a green efficient catalyzing way few in by-products is provided, and a technical basis is provided for preparation and promotion of theastaxanthin single body.

The invention belongs to the field of pig feed additives and particularly relates to a complex microbial agent and application thereof. The complex microbial agent is prepared from liquid spawns of the following strains: spore bacteria, actinomycetes, lactic acid bacteria, saccharomycetes, enterococcus and photosynthetic bacteria. The complex microbial fermented agent is used for preparing pig feed additives. The complex microbial agent can increase the lengths of stomachs, colons and ceca of fattening pigs, improve the richness of lactic acid bacteria of stomachs and colons, reduce productionof harmful clostridia major based on clostridium difficile, maintain intestinal structure functions, ensure flora balance and promote production performance, and can be applied to production as a feed additive.

The invention discloses a biological deodourant and a preparation method thereof, and belongs to the technical field of environmental protection. The biological deodourant is formed by mixing yeast liquid, spore bacteria liquid, lactic acid bacteria liquid, photosynthetic bacterial liquid and active enzyme liquid according to the mass ratio of (1-1.5):(1-1.5):(1-1.5):(0.05-0.07):(0.001-0.005); plant extraction liquid also can be added according to requirements; after the biological deodourant is used, antioxidants are generated within a short time; survival and reproduction of saprophytes arerestrained; a biological decomposition layer is formed on the surface of dirt; therefore, gaseous NH<3> cannot escape; microorganisms in the biological decomposition layer also can decompose harmful ingredients; the rapid deodorization effect is achieved; after pollutants are eaten, the microorganisms are decomposed into carbon dioxide, water and nontoxic cell debris; no secondary pollution exists; pungent smell cannot be generated in a use process; the biological deodourant can be in contact with the skin of a human body or an animal; furthermore, the biological deodourant is nonhazardous; and thus, the biological deodourant does not need to be cleaned specially.

The invention discloses bacillus firmus CX8 from a termite gut. The strain is collected in the China General Microbiological Culture Collection Center (CGMCC) with the address of No.3, No.1 Yard, Beichen West Road, Beijing 3 Chaoyang District on August 27th, 2018, and has a collection number of CGMCC No. 16342. The strain is gram-positive bacillus and is free of capsule, endogenous spores can be seen through spore staining, a bacterial colony is round and single, the color of the bacterial colony is white, and the edge of the bacterial colony is complete. The strain is a facultative anaerobicstrain for producing cellulase; and within 48 hours, the CMCase enzyme activity of the strain is 67.8 IU / mL, and the CX enzyme activity of the strain is 20.96 IU / mL. The strain not only has very highbiodiversity value, but also has scientific value for further research in development and application of cellulose-enriched crop and traditional Chinese medicine fermentation recycling technologies.

The invention discloses a microbial environment protectant, which is characterized by comprising mixed bacteria and a traditional Chinese medicine extract, wherein the mixed bacteria are formed by Rhodopseudomonas palustris, Bacillus Subtilis and Lactobacillus delbruckii. The weight ratio of the Rhodopseudomonas palustris to the Bacillus Subtilis to the Lactobacillus delbruckii is 3:4:7; the volume ratio of the traditional Chinese medicine extract to the mixed bacteria is 1-2 percent. The microbial environment protectant disclosed by the invention has the positive effects that through performing mixed fermentation on the compound bacterial liquid and the traditional Chinese medicine extract, the traditional Chinese medicine ingredients are further activated, and the traditional Chinese medicine ingredients and thallus exoenzyme interact to restrain the incubation of ova, and the generation of adult flies is reduced. As a traditional Chinese medicine and bacterial liquid fermentation medium is special in smell, a trapping and killing effect is performed on the adult flies, and the bad smell of a smelly source can also be effectively prevented from being emitted. The microbial environment protectant disclosed by the invention is harmless to people, is low in cost, simple in preparation process, simple and convenient in operation and remarkable in deodorization and fly killing effect. The problems that mosquitoes and the flies and foul smell exist in refuse landfills, waste transfer stations and digestion tanks are well solved, and a good effect is achieved.

The invention discloses the application of Bacillus subtilis in high-production of adenosine and co-production of acetoin. The Bacillus subtilis (Bacillus subtilis) is CGMCC No. 4484. The strains screened in the present invention can utilize a variety of carbon sources and nitrogen sources to ferment to produce adenosine and co-produce acetoin. By adding sodium citrate to the fermentation medium, the production of adenosine and acetoin can be significantly increased, and the operation is easy. It is simple, has extensive culture conditions, can maintain a high level in a 5L fermentation tank, and has the potential for large-scale industrialization.

The invention discloses preparation and application of active bacteria Bacillus sp for inhibiting the tobacco mildew rot, and relates to the technical field of microbial pesticides. A preparation process of the active bacteria Bacillus sp for inhibiting the tobacco mildew rot comprises the steps: inoculating NL-6 bacteria into a test tube LB liquid culture medium (5 ml per tube), wherein a formula of the culture medium comprises 0.5% of yeast extract, 1% of peptone, and 1% of NaCl; and inoculating the NL-6 thalli onto the culture medium, and culturing in a shaking table at the temperature of 37 DEG C for 12 hours at the rotating speed of 220rpm to obtain a test tube strain. The active bacterium Bacillus sp has the inhibitory activity on various pathogenic bacteria of the tobacco mildew rot, and after the activity of the active bacterium Bacillus sp is determined through multiple batches of activity tests for inhibiting the various pathogenic bacteria of the tobacco mildew rot, the strain is subjected to morphological, physiological, biochemical and molecular identification, and the active bacterium Bacillus sp is determined as a Bacillus sp spore bacterium.

The invention discloses an artificial synthetic antibacterial peptide with a beta-hairpin structure. The amino acid sequence is as follows: Cys-Ile-Cys-Arg-Arg-Gly-Phe-Cys-Val-Cys. Two arginines existin the corner position of the antibacterial peptide to form active sites where positive charges are concentrated; at the same time, a glycine exist in the corner position, so that the formation and stability of the corner structure are improved; four cysteines exist in the two arm positions to form two pairs of disulfide bonds; at the same time, hydrophobic amino acids exist in the two arm positions, so that the hydrophobicity of two arms is enhanced, and the insertion of the two arms into a bacterial cell membrane is facilitated; and the amidation design exist at the sequence C-terminus, sothat the charge influences at two ends of the sequence are reduced. Experiments show that the above antibacterial peptide provided by the invention can effectively inhibit or even kill staphylococcusaureus, escherichia coli, and aerobic spore bacteria, and has a good inhibitory effect on most of gram-positive bacteria.

The invention discloses a small-package sweetening agent utilizing bacillus. The small-package sweetening agent comprises the following ingredients: 0-0.1% of bacillus or 0-0.1% of clostridium butyricum, 0-10% of prebiotics and the balance sweetening agent. The small-package sweetening agent has the advantages that the bacillus probiotics can effectively resist an oxidation environment, and the activity of the probiotics can be effectively preserved even in a normal-temperature environment of a restaurant; the bacillus probiotics can effectively resist temperature damage of conventional hot drinks, most of the activity can be preserved even if the bacillus probiotics is mixed in coffee, hot milk, porridge, soup and the like with relatively high temperature, and most of the probiotics have the opportunity to arrive at intestinal tracts to germinate, so that positive influence is brought to human health; and after the probiotics are mixed with food and enter digestive tracts, compared with pure edible probiotics, microorganisms can show better activity due to no erosion of digestive juice, and more microorganism metabolites beneficial to health are generated.

The invention relates to the technical field of microorganisms, in particular to a bacillus velei and its application. The preservation number of Bacillus Velez Lpl-wx in the present invention is CGMCC No.17045. The bacterial strain can be applied to the preparation of astaxanthin esterase, and because the Bacillus velaisi belongs to prokaryote, the fermentation process is fast and the enzyme production efficiency is high, and the astaxanthin esterase can be obtained quickly and efficiently. The strain can be further applied to the preparation of astaxanthin monomers, which can effectively degrade astaxanthin esters and obtain astaxanthin monomers, providing a green and efficient catalytic pathway with less by-products, which is a good catalyst for the production of astaxanthin monomers. Preparation and promotion provide the technical basis.

The invention discloses a method for producing a biological feed by segmented solid fermentation of combined microorganisms. According to the invention, by adoption of segmented combination of mould,saccharomycetes, spore bacteria and lactic acid bacteria, and with cotton cake meal as a main substrate for solid fermentation, a cottonseed cake meal biological fermentation feed which has low free phenol, is rich in probiotics, protein, micromolecular peptide, lactic acid, biological enzyme and the like and facilitates digestion and absorption of animals is obtained. In the fermentation feed prepared according to a proportion, the content of free gossypol is 29 mg / kg; the content of micromolecular peptide is 18.7%; the content of amino acid nitrogen is 1.95%; the content of crude protein is37.1%, and is increased by 42% or above compared with the content of the crude protein in an initial culture medium; the number of lactic acid bacteria is 0.37 billion cfu / g; the number of spore bacteria is 5,300,000,000 cfu / g; the activity of protease is 267 u / g; the activity of glucoamylase is 390 u / g; and the activity of cellulase is 1.9 umol / g.