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Artificial synthetic antibacterial peptide with beta-hairpin structure

An artificial synthesis, hairpin structure technology, applied in the direction of antibacterial drugs, peptides, peptide/protein components, etc., can solve the problems of unstable structure and easy inactivation of natural antibacterial peptides, achieve good inhibitory effect, improve formation and stability , the effect of enhancing hydrophobicity

Inactive Publication Date: 2018-05-29
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, extracting natural antimicrobial peptides from organisms is the main idea of ​​antimicrobial peptide product development, but natural antimicrobial peptides have disadvantages such as unstable structure and easy inactivation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The amino acid sequence is the synthetic steps of Cys-Ile-Cys-Arg-Arg-Gly-Phe-Cys-Val-Cys antimicrobial peptide:

[0017] Fmoc-Rink-AM-Resin resin is fully swollen with DCM; take the first Fmoc-amino acid and dissolve it in DCM, then add DIEA and mix it into the reaction vessel, blow N2 to react for 2 hours, filter the reaction solution and add methanol, After the blocking reaction for 1 h, wash the resin with DCM, isopropanol and DMF respectively; then use 20% piperidine to remove Fmoc, wash thoroughly with DMF, add the second amino acid and mix with HOBt and DIEA for coupling, and react for 1 h Finally, use ninhydrin to detect that the reaction is complete if the resin is colorless and transparent, then fully wash with DMF; repeat the above steps until the last amino acid is completely reacted.

[0018] cutting:

[0019] After the resin is dry, break up the resin as much as possible, and transfer it to a chicken heart bottle equipped with a magnet, and slowly add 10 ...

Embodiment 2

[0022] Embodiment 2: the antibacterial activity assay of antimicrobial peptide

[0023] 1) Take 100 μl of bacteria cultured overnight from Staphylococcus aureus, Escherichia coli and Bacillus stearothermophilus respectively, dilute in 10ml of fresh culture medium, cultivate to the logarithmic growth phase (OD570 is 0.6-0.8), and then dilute to The OD value is 0.3 for standby;

[0024] 2) Filter the prepared nutrient solution with a 0.1 μm membrane. The nutrient solution consists of 20 mg bovine serum albumin, 2 mg yeast extract, 10 ml water, and 1 μL acetic acid;

[0025] 3) Dilute the antimicrobial peptide Cys-Ile-Cys-Arg-Arg-Gly-Phe-Cys-Val-Cys with the filtered nutrient solution by the doubling dilution method, and the final concentration is 10-320μM;

[0026] 4) Dilute the bacterial solution with an OD value of 0.3 100 times for use. Take a 96-well plate, add 180 μl fresh medium and 20 μl nutrient solution to the blank control group; add 180 μl bacterial solution and 20 ...

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PUM

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Abstract

The invention discloses an artificial synthetic antibacterial peptide with a beta-hairpin structure. The amino acid sequence is as follows: Cys-Ile-Cys-Arg-Arg-Gly-Phe-Cys-Val-Cys. Two arginines existin the corner position of the antibacterial peptide to form active sites where positive charges are concentrated; at the same time, a glycine exist in the corner position, so that the formation and stability of the corner structure are improved; four cysteines exist in the two arm positions to form two pairs of disulfide bonds; at the same time, hydrophobic amino acids exist in the two arm positions, so that the hydrophobicity of two arms is enhanced, and the insertion of the two arms into a bacterial cell membrane is facilitated; and the amidation design exist at the sequence C-terminus, sothat the charge influences at two ends of the sequence are reduced. Experiments show that the above antibacterial peptide provided by the invention can effectively inhibit or even kill staphylococcusaureus, escherichia coli, and aerobic spore bacteria, and has a good inhibitory effect on most of gram-positive bacteria.

Description

technical field [0001] The invention relates to the field of preparation of active peptides, in particular to an artificially synthesized antibacterial peptide with a beta-hairpin structure. Background technique [0002] There are a variety of pathogenic microorganisms in nature, which seriously affect human health and survival, and the usual way to resist pathogenic microorganisms is through the use of antibiotics. Although this method is simple and convenient, it is easy to lead to the enhancement of bacterial drug resistance, and it is often difficult to effectively eliminate it. The abuse, drug resistance, side effects, residues and other problems caused by the simultaneous use of antibiotics have become a major problem that plagues the development of human health. A variety of drug-resistant bacteria have been found, such as drug-resistant Enterobacteriaceae, Mycobacterium tuberculosis, and Methicillin-resistant Staphylococcus aureus (MRSA) known as "superbugs", which ...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P31/04A61K8/64A61Q17/00A23L3/3463
CPCA23L3/3463A23V2002/00A61K8/64A61K38/00A61Q17/005C07K7/06A23V2250/55Y02A50/30
Inventor 廖智
Owner ZHEJIANG OCEAN UNIV
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