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Spore protective agent and application thereof, and detection method of sporeformer producing content in microbial fertilizer

A technology of protecting agent and spores, which is applied in the field of microbial fertilizer detection, can solve the problem that the content of spores cannot be accurately known, and achieve the effect of ensuring the accuracy of detection.

Active Publication Date: 2014-11-19
湖南豫园生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a spore protective agent, its application and a detection method for the content of spore-producing bacteria in microbial fertilizers, so as to solve the technical problem that the content of spores existing in the form of spores in microbial fertilizers cannot be accurately known in the prior art

Method used

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  • Spore protective agent and application thereof, and detection method of sporeformer producing content in microbial fertilizer
  • Spore protective agent and application thereof, and detection method of sporeformer producing content in microbial fertilizer

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1) disperse the fertilizer to be tested (labeled bacteria content is 5,000,000,000 / gram) in spore protectant (sodium chloride concentration is 10% normal saline and normal saline 5wt.% polyaspartic acid, 1 drop of Tween ), configured into a bacterial suspension;

[0042] 2) Stir with a glass rod to make it dissolve and absorb water evenly, and then place the bacterial suspension in a water bath at 70°C for 15 minutes, then quickly cool it to 40°C, and fully shake it on a rotary shaker at 200r / min for 60 minutes to obtain a sterilized suspension;

[0043] 3) Dilute the sterilized suspension by 10 -7 Doubling inoculation into the spore medium, forming colonies after cultivation, and counting the number of colonies formed on the spore medium to obtain the bacterial content of the fertilizer to be tested;

[0044] Among them, the spore medium is composed of tryptone 6.0g / L, yeast extract powder 3.0g / L, glucose 1.5g / L, magnesium sulfate heptahydrate 1g / L, sodium chloride 1....

Embodiment 2

[0046] 1) Disperse the fertilizer to be tested (marked with a bacterial content of 4.5 billion / g) in a spore protectant (sodium chloride concentration of 8% in normal saline and 1wt.% polyaspartic acid in normal saline), configured as bacterial suspension;

[0047] 2) Stir with a glass rod to make it dissolve and absorb water evenly, and then place the bacterial suspension in a water bath at 75°C for 15 minutes, then rapidly cool to 30°C, and shake fully on a rotary shaker at 200r / min for 40 minutes to obtain a sterilized suspension;

[0048] 3) Dilute the sterilized suspension by 10 -7 Doubling inoculation into the spore medium, forming colonies after cultivation, and counting the number of colonies formed on the spore medium to obtain the bacterial content of the fertilizer to be tested;

[0049] Among them, the spore medium is composed of tryptone 4.0g / L, yeast extract powder 2.0g / L, glucose 0.5g / L, magnesium sulfate heptahydrate 0.2g / L, sodium chloride 0.5g / L, calcium car...

Embodiment 3

[0051] 1) Disperse the fertilizer to be tested (marked with a bacterial content of 4 billion / g) in a spore protectant (a sodium chloride concentration of 8.5% in normal saline and 4wt.% polyaspartic acid in normal saline), configured as bacterial suspension;

[0052] 2) Stir with a glass rod to make it dissolve and absorb water evenly, then place the bacterial suspension in a water bath at 80°C for 15 minutes, quickly cool it to 35°C, and fully shake it on a rotary shaker at 200r / min for 50 minutes to obtain a sterilized suspension;

[0053] 3) Dilute the sterilized suspension by 10 -7 Doubling inoculation into the spore medium, forming colonies after cultivation, and counting the number of colonies formed on the spore medium to obtain the bacterial content of the fertilizer to be tested;

[0054] Among them, the spore medium is composed of tryptone 5.0g / L, yeast extract powder 2.5g / L, glucose 1.0g / L, magnesium sulfate heptahydrate 0.5g / L, sodium chloride 1.0g / L, calcium carb...

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Abstract

The invention provides a spore protective agent and application thereof, and a detection method of sporeformer producing content in a microbial fertilizer. The spore protective agent comprises normal saline with the sodium chloride concentration of 8-10% and polyaspartic acid which accounts for 1-5 wt.% of the normal saline. The sporeformer protective agent can protect the germinated spores, so that the spores can continue growth in the culture medium within the sterilization temperature of 70 DEG C, thereby ensuring the detection accuracy of the sporeformer content in the microbial fertilizer in the subsequent process.

Description

technical field [0001] The invention relates to the field of detection of microbial fertilizers, in particular to a spore protective agent, its application and a detection method for the content of spore-forming bacteria in microbial fertilizers. Background technique [0002] In the prior art, the detection standard of the Ministry of Agriculture has always been used for the detection of the number of viable bacteria in microbial fertilizers. The detection method is as follows: Dilute the bacterial agent products, put the diluted bacterial solution to a certain concentration into the pre-prepared plate detection medium, after cultivation, observe and count the number of colonies in the medium with naked eyes, and then calculate The number of bacteria contained in the product. In order to ensure the accuracy of the detection, it is necessary to select the corresponding medium for cultivation according to the types of bacteria contained in the fertilizer. [0003] There are ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/06C12R1/07
Inventor 郑锦华陈剑黄旭明李庆红谭欢袁新荣苏红
Owner 湖南豫园生物科技股份有限公司
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